NANOG Recombinant Monoclonal Antibody

Code: CSB-RA888008MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, FC

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Target Background

Product Details

Uniprot NO.

Q9H9S0

Target Names

NANOG

Alternative Names

Embryonic stem cell specific homeobox protein (Nanog) antibody; ENK antibody; FLJ12581 antibody; hNanog antibody; Homeobox protein NANOG antibody; Homeobox transcription factor Nanog antibody; homeobox transcription factor Nanog-delta 48 antibody; NANOG antibody; Nanog homeobox antibody; NANOG_HUMAN antibody

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human NANOG protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

8A1D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HeLa whole cell lysate(20µg), HepG2 whole cell lysate(20µg), A2780 whole cell lysate(20µg), Mouse Brain tissue lysate(20µg)
All lanes: NANOG antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time：5min
Overlay Peak curve showing HepG2 cells stained with CSB-RA006327MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

NANOG is a master transcription factor that plays a central role in maintaining pluripotency and self-renewal capacity in embryonic stem cells. This homeobox protein works in concert with other key regulators like OCT4 and SOX2 to sustain the undifferentiated state, making it an essential marker for stem cell research, developmental biology, and studies investigating cellular reprogramming. Its expression patterns also hold significance in cancer research, where aberrant NANOG activity has been linked to tumor-initiating cell populations.

This recombinant monoclonal antibody against NANOG offers the consistency and reliability that demanding stem cell experiments require. Because the antibody sequence is defined and produced recombinantly, you can expect identical performance across lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations. The human IgG1 format and affinity-chromatography purification ensure high specificity for your target.

Validation studies demonstrate robust performance across multiple applications. In Western blot analysis, this antibody detects a band at the predicted 35 kDa molecular weight in HeLa, HepG2, and A2780 human cell lysates, as well as mouse brain tissue lysate, confirming cross-species reactivity with both human and mouse samples. Flow cytometry validation in HepG2 cells shows clear separation between antibody-stained and isotype control populations, indicating reliable intracellular detection of NANOG in fixed and permeabilized cells.

Whether you are characterizing pluripotent stem cell populations, investigating differentiation pathways, or exploring NANOG's role in cancer stem cell biology, this antibody provides the flexibility to move seamlessly between Western blot and flow cytometry workflows with confidence in your results.

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Target Background

Function(From Uniprot)

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Gene References into Functions

The critical roles of NANOG and its pseudogene NANOGP8 in cancer progression leads the association of these genes with exosomes to be significant, and may allow for exosomal NANOG to function as a powerful diagnostic biomarker. Variations in NANOG/NANOGP8 gene sequences in exosomal DNA includes an insertion into the 3' UTR. PMID:29787607
A higher expression of the pluripotency factor NANOG. PMID:29845283
The interaction of Nanog with the AR signaling axis might induce or contribute to Ovarian cancer stem cells regulation. In addition, androgen might promote stemness characteristics in ovarian cancer cells by activating the Nanog promoter PMID:29716628
The results show that Nanog expression was related to HBsAg, differentiation, and TNM stage in hepatocellular carcinoma (HCC). Nanog may be an unfavorable prognostic biomarker for HCC. PMID:29198990
NANOG might be a potential biomarker for early diagnosis of urothelial carcinoma of the bladder. PMID:29279584
These results demonstrate that analysis of IHC expression patterns of MK and NANOG in pretreatment biopsy specimens during the work-up period can provide a more definitive prognosis prediction for each oral squamous cell carcinoma (OSCC) patient that can help clinicians to develop a more precise individual treatment modality. PMID:29113102
Chicken egg-white extracts promote OCT4 and NANOG expression and telomeres growth in 293T cells. PMID:28838341
Rectal tumor tissue OCT4 (p<0.001), SOX2 (p=0.003), and NANOG (p<0.001) expressions were higher than those in adjacent tissue. PMID:29214774
Data show that lung adenocarcinoma SPC-A1 cell differentiated by a two-stage induction (TSI) method lost stem cell characteristics, including absent expression of OCT4 and Nanog. PMID:27588392
our data suggest that 3D culture increases the expression of Nanog through the relaxation of actin cytoskeleton, which mediates reduced Suv39h1 and H3K9me3 levels. PMID:28276635
results show that NANOG could reverse the effects of stem cell senescence and restore the myogenic differentiation potential of senescent MSCs. PMID:28125933
MACC1-induced tumor progression in colorectal cancer acts, at least in part, via the newly discovered MACC1/Nanog/Oct4 axis. PMID:26758557
RNAi-mediated silencing of NANOG in SKOV-3 reversed the expression of mesenchymal cell markers and restored expression of E-cadherin. Susceptibility to cisplatin increased in SKOV-3 cells on down-regulating NANOG and reversible results were obtained in Moody cells post-overexpression of NANOG. PMID:27884977
NANOG enabled reactivation of the ROCK and Transforming Growth Factor (TGF)-beta pathways-both of which were impaired in senescent cells-leading to ACTIN polymerization, MRTF-A translocation into the nucleus and serum response factor (SRF)-dependent myogenic gene expression. PMID:27350449
High NANOG expression is associated with brain neoplasms. PMID:28933914
Super-enhancers at the Nanog locus differentially regulate neighboring pluripotency-associated genes, in particular, DPPA3. PMID:27681417
our data reveal that SATB2 in alveolar bone mesenchymal stem cells (AB-BMSCs) associates with their age-related properties, and prevents AB-BMSCs senescence via maintaining Nanog expression. PMID:27632702
High NANOG expression is associated with Multidrug Resistance in breast and cervical cancer. PMID:28716899
miR-612 has a suppressive role on hepatocellular carcinoma cell stemness via Sp1/Nanog signaling pathway. PMID:27685621
These data reveal an overexpression of NANOG and other markers of pluripotency and stemness in meningiomas. PMID:28345785
The NANOG deficiency affected multiple genes, particularly, supressed drug-resistance via down-regulated ABCG2 in Eca109 cells, and caused G1 arrest by down-regulated cyclin D1 (CCND1) expression. PMID:28601640
Endogenous Plastic Somatic (ePS) cells in a latent state, i.e. lacking SOX2, OCT3/4 and NANOG (SON) expression, in non-diseased breast specimens through immunohistochemical analysis of previously identified ePS-specific biomarkers (CD73(+), EpCAM(+) and CD90(-)). PMID:27705752
Data suggest that C-terminal truncated hepatitis B virus X protein (HBx-DeltaC1) enhances liver cancer stem cell (CSC) properties through Stat3/Nanog cascade, and insight for the therapeutic intervention for hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). PMID:28186991
Nanog directly repressed transcription of the miR-200c and miR-200b genes in colon cancer cells, inducing epithelial-mesenchymal transformation. PMID:28163188
LGR5-expressing fraction of CD54+ cells represents gastric cancer CSCs, in which LGR5 is closely associated with stemness and EMT core genes PMID:28033430
Data show that Nanog homebox (NANOG) but not sex-determining region Y-box2 (SOX2) and octamer-binding protein 4 (OCT4) expression was overexpressed in the endometrium of women with intrauterine adhesion (IUA). PMID:28253866
The NANOG transcription was significantly upregulated by ETV4 overexpression. PMID:28412366
Collectively, these findings demonstrate a novel role of YBX1 in maintaining the stemness of CSCs and metastasis, unveiling YBX1 as promising therapeutic target for NSCLC treatments. PMID:28400280
the early response of pluripotency genes OCT4 and NANOG to the differentiation-inducing stimuli is mediated by dynamic changes in chromatin marks, while DNA methylation is acquired in the later stages of neurogenesis. PMID:28601081
USP21 specifically regulates the Lys48-linked polyubiquitination and stability of NANOG PMID:27956178
To our knowledge, this is the first report on lineage reprogramming of TILs using protein stem cell transcription factors delivered directly to the nucleus. PMID:27084449
Nanog expression is a prognostic biomarkers for triple-negative breast cancer PMID:27300169
Stat3 was correlated with NANOG-mediated EMT. PMID:26801672
miR-760 was proved to be functional associated with NANOG via regulating its expression. PMID:27133070
SIRT-1 and NANOG are high correlated biological markers for diagnosis and prognosis follow up in patients with adenocarcinoma. PMID:27540973
renal cell carcinoma patients with low Nanog and Oct4 expressions in tumor tissues had significantly higher survival rates (p < 0.05). High Nanog and Oct4 expressions may be potential therapeutic targets. PMID:26631537
ANOG was regulated by extracellular IGF signaling pathway via STAT3 phosphorylation in colorectal cancer (CRC). This coincides with that IGF receptor IGF-1R is often increasing expressed in malignant metastasis colon cancer. Taken together, our data define the crucial functions of IGF/STAT3/NANOG/Slug signaling axis in the progression of CRC PMID:26840943
Nanog is a positive regulator of cervical cancer dedifferentiation. PMID:26936116
Data show that long intergenic non-protein coding RNA ROR may act as a competitive endogenous RNAs (ceRNAs), effectively becoming a sink for microRBA miR-145, thereby activating the derepression of core transcription factors Nanog. PMID:26636540
ALKBH5 overexpression decreased NANOG mRNA methylation, increased NANOG levels, and increased the percentage of BCSCs, phenocopying the effect of hypoxia PMID:27001847
Expression levels of OCT4, SOX2, and NANOG were evaluated by immunohistochemistry with tissue microarray slides of 436 OSCC, 362 corresponding tumor-adjacent normal (CTAN) tissues, and 71 normal uvula epithelium tissues. PMID:26211876
Nanog possesses important function in BCSC. PMID:26339994
Our study provides new insight into the function of DPPA5 and NANOG regulation in human pluripotent stem cell . PMID:26661329
the available data demonstrate that NANOG is strictly involved in the process of carcinogenesis and is a potential prognostic marker of malignant tumors. PMID:26618281
the disruption of Nanog expression results in less proliferation, invasiveness, migration, more chemosensitivity and reversal of EMT in HepG2 cells, by which Nanog plays crucial roles in influencing the malignant phenotype of HepG2 cells. PMID:26676719
The promoter activity of Nanog and Oct4 were upregulated, and beta-catenin was observed to bind to these promoters during H. pylori infection, while a Wnt/beta-catenin inhibitor suppressed promoter activity and binding. PMID:26940070
ectopic expression of Oct-4 gene in hAFMSCs with high self-renewal ability could upregulate Nanog and Sox-2 gene expression PMID:25385323
Nanog is an oncogene with multiple roles in promoting tumorigenesis and metastasis PMID:26073077
The positive correlation among Oct-4, Nanog, and beta-catenin suggests their coordinated role in maintaining proliferation in oral carcinoma cells. PMID:24700702
Oct3/4 and Nanog represent probable CSC markers in HNSCC, which contribute to the development of DNM in part by enhancing cell motility and invasiveness. PMID:26483189

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Subcellular Location

Nucleus.

Protein Families

Nanog homeobox family

Tissue Specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and

Database Links

HGNC: 20857

UNIGENE: Hs.635882

KEGG: hsa:79923

STRING: 9606.ENSP00000229307

OMIM: 607937

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