LEFTY2 Recombinant Monoclonal Antibody

Code: CSB-RA171603A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

O00292

Target Names

LEFTY2

Alternative Names

Left-right determination factor 2 (Endometrial bleeding-associated factor) (Left-right determination factor A) (Protein lefty-2) (Protein lefty-A) (Transforming growth factor beta-4) (TGF-beta-4), LEFTY2, EBAF LEFTA LEFTYA TGFB4

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human LEFTY2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10G2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Ntera-2 whole cell lysate, Hela whole cell lysate
All lanes: LEFTY2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41, 38 kDa
Observed band size: 36-45 kDa

Description

The process of creating the LEFTY2 recombinant monoclonal antibody involves multiple stages. First, the LEFTY2 monoclonal antibody is harvested and its gene sequence is determined. Next, a vector carrying the LEFTY2 monoclonal antibody gene is constructed and transfected into a host cell line for culture. During the production of the LEFTY2 monoclonal antibody, a synthesized peptide from human LEFTY2 is used as the immunogen. Once produced, the LEFTY2 recombinant monoclonal antibody is purified using affinity chromatography. Finally, the specificity of the LEFTY2 recombinant monoclonal antibody is evaluated through ELISA and WB applications.

LEFTY2 protein is a secreted protein that plays a key role in embryonic development, specifically in the determination of left-right asymmetry. It is a member of the transforming growth factor-beta (TGF-β) superfamily and is expressed in the left lateral plate mesoderm during early development. LEFTY2 is involved in the regulation of nodal signaling, which is essential for the establishment of left-right asymmetry. It acts as an antagonist to nodal signaling by binding to nodal proteins and inhibiting their activity, thereby promoting left-sidedness. Additionally, LEFTY2 has been shown to have anti-tumor effects in certain cancers.

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Target Background

Function(From Uniprot)

Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.

Gene References into Functions

functional role of LEFTY during progesterone therapy for endometrial carcinoma PMID:29268772
LeftyA leads to disruption of Rac1 and Pak1 activity with subsequent actin depolymerization, cell softening and cell shrinkage. PMID:27404958
LEFTY2 down-regulates MKi67 expression and FAK activity, up-regulates miR-200a and E-cadherin, and is thus a powerful negative regulator of endometrial cell proliferation and migration. PMID:27497669
LEFTY2 regulates the expression and activity of ENaC in endometrial epithelial cells via SGK1. PMID:27606670
Lefty A may account for the tumor suppressive activity of human adult stem cells. PMID:22469982
Findings suggest that Lefty2 is negatively modulated by miR-302s in hESCs, which plays an important role in maintaining the balance between pluripotency and germ layer specification. PMID:21266536
Findings show that during embryonic development, EBAF/LEFTY B plays important roles in decidualization and embryo implantation. PMID:21401636
findings show that the TGFB4 (ebaf) mRNA has distinct tumor specific expression PMID:9230066
may provide a crucial signal for endometrial breakdown and bleeding by triggering expression of several matrix metalloproteinases PMID:12215426
nodal and the inhibitors of Nodal signaling, lefty-A and lefty-B, are down-regulated very early upon differentiation of human embryonic stem cells PMID:15308665
Addition of recombinant LEFTY-A to explants induced MMP-9 in most samples, a response prevented by ovarian steroids. PMID:15536155
LEFTY proteins, which are known to play a major role during mouse gastrulation, are transiently expressed during human embryonic stem cell differentiation. PMID:17038673