LDHC Recombinant Monoclonal Antibody

Code: CSB-RA886298A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P07864

Target Names

LDHC

Alternative Names

L-lactate dehydrogenase C chain (LDH-C) (EC 1.1.1.27) (Cancer/testis antigen 32) (CT32) (LDH testis subunit) (LDH-X), LDHC, LDH3 LDHX

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human LDHC

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9F2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing Hela cells stained with CSB-RA886298A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

The generation of the LDHC recombinant monoclonal antibody involves a genetic engineering process that includes cloning and expression of the gene responsible for the LDHC monoclonal antibody. To generate the LDHC monoclonal antibody, a synthesized peptide derived from human LDHC protein was used as the immunogen. The LDHC recombinant monoclonal antibody was purified using affinity chromatography to ensure high purity. It specifically recognizes and binds to the human MAS1L protein. The quality and specificity of the LDHC recombinant monoclonal antibody have been confirmed through two applications ELISA and FC.

LDHC is an enzyme that plays a crucial role in the anaerobic metabolism of glucose. It catalyzes the reversible conversion of pyruvate to lactate, producing NAD⁺ from NADH. This process is important in energy production during periods of high-intensity exercise when oxygen availability is limited. LDHC is predominantly expressed in the testis, where it is essential for the process of spermatogenesis. However, it is also expressed in other tissues, such as muscle and brain, where it may have other functions beyond energy metabolism. Mutations in the LDHC gene have been associated with male infertility.

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Target Background

Function(From Uniprot)

Possible role in sperm motility.

Gene References into Functions

Lactate dehydrogenase C may act as a novel biomarker for renal cell carcinoma progression and a potential therapeutic target for the treatment of renal cell carcinoma. PMID:28351304
three uniquely identified proteins (CDK6 , galectin-3-binding protein and LDH C) were found, which show tight connection with prostate cancer and presence of all of them was previously linked to certain aspects of prostate cancer PMID:26503549
Down-regulated LDH-C4 expression is significantly correlated with lowered enzyme activity in human spermatozoa. PMID:25795631
This is the first report to show a positive correlation between LDH-3 and hemolytic parameters in sickle cell anemia. PMID:26069337
LDH3 was increased in essential thrombocythemia. This isoenzymatic pattern could be expression of a metabolic adaptation. PMID:17178662
LDH3 is a supporting diagnostic marker in cases of chronic tuberculosis. PMID:17935709
hLdhc expression in cancer cells was regulated by transcription factor Sp1 and CREB and promoter CGI methylation PMID:18930904