LDHA Recombinant Monoclonal Antibody

Code: CSB-RA907805A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P00338

Target Names

LDHA

Alternative Names

Cell proliferation-inducing gene 19 protein antibody; GSD11 antibody; L lactate dehydrogenase B chain antibody; L-lactate dehydrogenase A chain antibody; Lactate dehydrogenase A antibody; Lactate dehydrogenase B antibody; Lactate dehydrogenase c variant 1 antibody; Lactate dehydrogenase c variant 3 antibody; Lactate dehydrogenase c variant 4 antibody; Lactate dehydrogenase C4 antibody; Lactate dehydrogenase H chain antibody; Lactate dehydrogenase M antibody; LDH A antibody; LDH B antibody; LDH H antibody; LDH heart subunit antibody; LDH M antibody; LDH muscle subunit antibody; LDH-A antibody; LDH-M antibody; LDH1 antibody; ldha antibody; LDHA_HUMAN antibody; LDHBD antibody; LDHM antibody; MS1111 antibody; PIG19 antibody; Proliferation inducing gene 19 antibody; Proliferation-inducing gene 19 antibody; Renal carcinoma antigen NY REN 46 antibody; Renal carcinoma antigen NY-REN-59 antibody; TRG 5 antibody; TRG5 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human LDHA

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HepG2 whole cell lysate(20µg), Hela whole cell lysate(20µg),Raji whole cell lysate(20µg),HEK293 whole cell lysate(20µg),A431 whole cell lysate(20µg),MCF-7 whole cell lysate(20µg),NIH/3T3 whole cell lysate(20µg)
All lanes: LDHA antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time：2min
IHC image of CSB-RA907805A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing 786-O cells stained with CSB-RA907805A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Lactate dehydrogenase A (LDHA) serves as a pivotal enzyme in cellular metabolism, catalyzing the interconversion of pyruvate and lactate during anaerobic glycolysis. This metabolic gatekeeper has garnered significant research attention due to its elevated expression in rapidly proliferating cells and its established role in the Warburg effect, making it a compelling target for cancer metabolism studies and therapeutic development.

This recombinant monoclonal antibody against LDHA offers researchers the reproducibility advantages inherent to recombinant technology. Generated from a defined sequence and produced as clone 3E9, it delivers the lot-to-lot consistency essential for longitudinal studies and multi-site collaborations where experimental reproducibility is paramount.

Extensive validation across multiple platforms demonstrates this antibody's versatility in diverse experimental workflows. Western blot analysis confirms robust detection of LDHA at the expected 37 kDa molecular weight across an impressive range of human cell lines, including HepG2, HeLa, Raji, HEK293, A431, and MCF-7, as well as the mouse NIH/3T3 line, confirming the antibody's cross-species reactivity with both human and mouse samples. Immunohistochemistry validation in paraffin-embedded human stomach tissue showcases its utility for examining LDHA expression patterns in archival clinical specimens. Flow cytometry analysis using 786-O renal carcinoma cells further extends its application to single-cell studies, with clear separation from isotype control demonstrating specific intracellular detection.

The unconjugated format and affinity-purified preparation provide flexibility for researchers to pair this antibody with their preferred detection systems. Whether investigating metabolic reprogramming in cancer, exploring glycolytic flux in immune cells, or characterizing LDHA expression in tissue samples, this antibody delivers the specificity and consistency that rigorous research demands.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

The study reveals that miR-33b plays a suppressive role in the regulation of osteosarcoma cell proliferation through direct targeting LDHA. PMID:30213286
hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation. PMID:28516914
the results of the present study demonstrated that miR199a3p can inhibit LDHA expression by downregulating Sp1, and provided mechanistic evidence supporting the existence of a novel miR199a3p/Sp1/LDHA axis and its critical contribution to aerobic glycolysis in testicular cancer cells. PMID:30015851
High LDHA expression is associated with Non-Small Cell Lung Cancer. PMID:29321091
This is the first study from this highly prevalent region of Head neck cancer showing that serum LDH could be regarded as a biomarker for malignant and premalignant conditions of the head and neck. PMID:30197328
High LDH expression is associated with small cell lung cancer. PMID:29970686
The PFK2 expression along with LDH-4 were observed to be increased ~2-fold (P < 0.001) in 0.5 mM ammonia treated brain slices. PMID:23703029
miR-199a-3p is a major metabolic regulator in tumor suppression and miR-199a-3p may downregulate metabolic genes (LDHA, PGK1, MCT1, TIGAR) through the transcription factor Sp1. PMID:27432288
The expression of LDH-5 and hypoxia-inducible factor (HIF) 1alpha in Non-Hodgkin's lymphoma, was examined. PMID:24086384
Results show that LDHA is highly expressed in triple negative breast cancer (TNBC) and correlated with a poor outcome. Its 3'UTR is targeted by miR-34a. Also, LDHA along with PDL1 seem to act as ceRNAs to promote the expression and function of each other through regulation of miR-34a in TNBC. PMID:28915924
LDHA siRNA can inhibit cell proliferation, induce apoptosis, reduce invasion and migration in renal cell carcinoma cells. PMID:27027898
JMJD2A regulated aerobic glycolysis by regulating LDHA expression. Therefore, the novel JMJD2A-LDHA signaling pathway could contribute to the Warburg effects in NPC progression. PMID:28693517
The present study proved that HIF1/2alpha could activate LDHA expression in human pancreatic cancer cells, and high expression of LDHA promoted the growth and migration of pancreatic cancer cells. PMID:28193910
Pretreatment LDH levels had noticeable prognostic value in advanced pancreatic ductal adenocarcinoma (PDAC) patients who received subsequent chemotherapy. Tackling elevated LDH levels before the initiation of chemotherapy might be a promising measure for improving overall survival of patients after treatment for their advanced PDAC. PMID:28056913
Authors demonstrated that knock down of LDHA with siRNA or inhibition of LDHA activity with a LDHA specific inhibitor (FX-11), could sensitize PC-3RR cells to radiotherapy with reduced epithelial-mesenchymal transition, hypoxia, DNA repair ability and autophagy, as well as increased DNA double strand breaks and apoptosis. PMID:27708237
Taken together, these results indicate that miR-142-3p could act as a tumor suppressor in HCC by targeting LDHA, suggesting new therapeutic targets for HCC treatment. PMID:29360449
Findings suggest that miR-34b-3 and miR-449a suppress the development of nasopharyngeal carcinoma (NPC) through regulation of glycolysis via targeting lactate dehydrogenase A.(LDHA) and may be potential therapeutic targets for the treatment of NPC. PMID:27458165
Results provide evidence that miR-200b acts as a tumor suppressor in glioma through the inhibition of LDHA both in vitro and in vivo. PMID:27374173
our data demonstrate that overexpression of 14-3-3zeta in early stage pre-cancerous breast epithelial cells may trigger an elevated glycolysis and transcriptionally up-regulating LDHA, thereby contributes to human breast cancer initiation. PMID:27150057
serum lactate dehydrogenase has a role in predicting the outcome of resected gastric cancer and construct prognostic nomograms for risk prediction PMID:27223065
LDHA phosphorylation and activation provide pro-invasive, anti-anoikis and pro-metastatic advantages to cancer cells, suggesting that Y10 phosphorylation of LDHA may represent a promising therapeutic target and a prognostic marker for metastatic human cancers. PMID:28218905
miR-30a-5p may function as a tumor suppressor in breast cancer through the inhibition of LDHA expression and glycolysis. PMID:28461244
Overexpression of LDHA can be a marker of poor prognosis in cholangiocarcinoma patients PMID:27615379
LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and natural killer cells. PMID:27641098
Data indicate that lactate dehydrogenase A (LDHA) is involved in the transcription of histone 2B gene. PMID:28257841
LDHA downregulation is involved in the apoptotic effect of diallyl trisulfide in triple-negative breast cancer. PMID:27566995
this study shows that low levels of LDH correlate to a better anti-CTLA-4 immunotherapy response and survival in advanced melanoma patients PMID:27728898
alternative isoforms of LDH in cancer cells produce lactic acid, when LDHA is silenced or inhibited PMID:28314283
Overexpression of PKM2 and LDH5 associates with key clinicopathological features and unfavourable prognosis in tongue squamous cell carcinoma. PMID:24762230
These results suggest LDH-A and/or lactate as common elements at the cross-road between cancer cell metabolism, tumor progression and inflammation PMID:27622920
High serum LDH is associated with OS and PFS in patients with urinary system cancer, and it is an effective biomarker of prognosis in patients with urologic cancer. PMID:27710971
High serum LDH level is associated with cancer. PMID:27511116
The number of in advanced-stage diffuse large B cell lymphoma patients with elevated serum lactate dehydrogenase who achieved complete response (CR) after R-CHOP in the non autologous hematopoietic stem cell transplantation (ASCT) group was higher than that in the ASCT group. PMID:27324387
this study shows that elevated serum LDH levels are associated with unfavorable prognosis in nasopharyngeal carcinoma patients treated with intensity-modulated radiotherapy PMID:26928265
LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer. PMID:26902416
IDO, through GCN2 kinase activation, downregulates the levels of TCRcomplex tchain and cMyc, resulting in the suppression of Tcell proliferation and a reduction in the levels of LDHA and GLS2 PMID:26647830
Findings provide evidence of the cellular functions of lactate dehydrogenase A in the progression of glioblastoma and suggest that lactate dehydrogenase A might act as a potential therapeutic target for glioblastoma treatment. PMID:26694942
Reanalysis of genome-wide association study and in vitro validation show that lactate dehydrogenase interacts with branched-chain amino acid metabolism. PMID:26014429
LDH and complex I play distinct roles in regulating glycolysis and cell proliferation. Inhibition of these two augments synthetic lethality in melanoma. PMID:26484566
Multivariable analysis showed that combined expression of pyruvate kinase type M2 and lactate dehydrogenase A was an independent poor prognostic marker for survival. PMID:26989901
Although lactate dehydrogenase A (LDHA) expression varies biphasically during melanoma brain metastasis formation, tumor progression and survival seem to be functionally independent of LDHA. PMID:25791837
dehydrogenase A negatively regulated by miRNAs promotes aerobic glycolysis and is increased in colorectal cancer. PMID:26062441
positive relationship between LDH-A expression and CSC/EMT markers was confirmed both in invasive bladder cell line and in 136 MIBC specimens. PMID:26721441
FOXM1-LDHA signaling functioned as a stimulator of glycolysis and promoted gastric cancer progression. PMID:26261559
Mammalian photoreceptors contain dimeric and tetrameric PKM2 and LDH-A. This is consistent with the ability to switch between energy production and biosynthesis like a proliferating tissue, possibly due to demands of opsin synthesis. PMID:26780311
We propose a profibrotic feed forward loop, in which radiation induces LDHA expression and lactate production, which can lead to further activation of TGF-beta to drive the fibrotic process. PMID:26254422
These results indicate that disrupting SLC2A1, LDHA, or other regulators in cancer cell energetics is a very promising approach for new targeted therapies. PMID:25838393
High expression of LDHA is associated with gastric cancer. PMID:25913622
High LDHA expression is associated with tumor progression in prostate cancer. PMID:25983002
LDHA is downregulated by JQ1, which suppresses tumor growth in ovarian cancer PMID:25762632