KDM5A Recombinant Monoclonal Antibody

Code: CSB-RA830757A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P29375

Target Names

KDM5A

Alternative Names

Histone demethylase JARID1A antibody; JARID1A antibody; Jumonji/ARID domain containing protein 1A antibody; Jumonji/ARID domain-containing protein 1A antibody; Kdm5a antibody; KDM5A_HUMAN antibody; Lysine-specific demethylase 5A antibody; RBBP-2 antibody; RBBP2 antibody; RBP2 antibody; Retinoblastoma binding protein 2 antibody; Retinoblastoma-binding protein 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human KDM5A protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of Hela cell with CSB-RA830757A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing Hela cells stained with CSB-RA830757A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

KDM5A, also known as JARID1A or RBP2, functions as a histone demethylase that specifically removes methyl groups from tri- and di-methylated lysine 4 on histone H3. This enzymatic activity plays a central role in transcriptional regulation, with KDM5A serving as a key modulator of gene expression programs involved in cell cycle control, differentiation, and cellular senescence. Originally identified through its interaction with the retinoblastoma tumor suppressor protein, KDM5A has emerged as an important factor in cancer biology, where its dysregulation contributes to altered epigenetic landscapes in multiple tumor types.

This recombinant monoclonal antibody, clone 23B11, offers the reproducibility and consistency that demanding epigenetics research requires. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human KDM5A, provides reliable detection in human samples.

Validation studies demonstrate robust performance in both immunofluorescence and flow cytometry applications. Immunofluorescence analysis of HeLa cells reveals clear nuclear localization consistent with KDM5A's chromatin-associated function, with optimal staining achieved at dilutions between 1:50 and 1:200. Flow cytometry validation in HeLa cells confirms specific detection with distinct signal separation from isotype controls, supporting quantitative analysis of KDM5A expression at the single-cell level.

For researchers investigating histone modification dynamics, transcriptional regulation, or the epigenetic mechanisms underlying disease states, this antibody provides a dependable tool for characterizing KDM5A expression and localization across experimental systems.

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Target Background

Function(From Uniprot)

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1. Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells.

Gene References into Functions

ZEB1 play a crucial role in KDM5A induced function. PMID:29324315
both KDM5A and KDM5B are involved in the lengthening of DICER1 PMID:28138513
RBP2 promotes HIF-1alpha-VEGF-induced angiogenesis of non-small cell lung cancer via the Akt pathway. PMID:25162518
promotes overexpression and activation of BCL2 in acute lymphoblastic leukemia development and progression PMID:27008505
Ectopic overexpression of RBP2 can induce cancer stem cell-like (CSC) phenotypes through epithelial to mesenchymal transition in renal cell carcinoma cells by converting them to a more mesenchymal phenotype. PMID:27282106
miR-34a promotes the osteogenic differentiation of human adipose-derived stem cells via the RBP2/NOTCH1/CYCLIN D1 coregulatory network. PMID:27453008
This study aimed to explore the expression level of RBP2 in hepatocellular carcinoma (HCC) and its prognostic significance. PMID:28582381
KDM5A suppresses ovarian cancer cell apoptosis under paclitaxel treatment. PMID:28714030
KDM5A 5A inhibitor blocks cancer cell growth and drug resistance PMID:27224921
KDM5A-mediated H3K4me3 modification participated in the etiology of osteoporosis and may provide new strategies to improve the clinical efficacy of BMP2 in osteoporotic conditions. PMID:27512956
KDM5A demethylates H3K4 to allow ZMYND8-NuRD to operate within damaged chromatin to repair DNA double strand breaks. PMID:28572115
the radiation sensitivity observed following depletion of Jarid1A is not caused by a deficiency in repair of DNA double-strand breaks. PMID:27253695
Treatment-induced temozolimide resistance in glioblastoma cells involves KDM5A mediated epigenetic mechanisms. PMID:26566863
Characterization of a Linked Jumonji Domain of the KDM5/JARID1 Family of Histone H3 Lysine 4 Demethylases. PMID:26645689
KDM5A is regulated by its reader domain through a positive-feedback mechanism PMID:25686748
Data indicate that lysine (K)-specific demethylase 5A RBP2 (JARID1A; KDM5A) epigenetically downregulated micrRNA-21 (miR-21) in blast transformation of chronic myeloid leukemia (CML). PMID:25575817
LSD1 is a more sensitive molecular marker than RBP2 on thyroid cancer diagnosis. PMID:24068396
MiR-212 directly regulates the expression of RBP2 and inhibits cell growth in gastric cancer, which may provide new clues to treatment. PMID:23794145
RBP2 is critical for breast cancer metastasis to the lung in multiple in vivo models. Mechanistically, RBP2 promotes metastasis as a pleiotropic positive regulator of many metastasis genes, including TNC. PMID:24582965
RBP2 is overexpressed in HCC and negatively regulated by hsa-miR-212. The hsa-miR-212-RBP2-CDKI pathway may be important in the pathogenesis of HCC. PMID:23922798
Overexpression of RBP2 and activation of VEGF might play important roles in human gastric cancer development and progression. PMID:24716659
RBP2 is overexpressed in gastric cancer, and its inhibition triggers senescence of malignant cells at least partially by derepressing its target genes cyclin-dependent kinase inhibitors. PMID:19850045
our results establish an oncogenic role for RBP2 in lung tumorigenesis and progression and uncover novel RBP2 targets mediating this role. PMID:23722541
RBP2 down-regulated the expression of E-cadherin, up-regulated the expression of N-cadherin and snail, and induced epithelial-mesenchymal transition in non-small cell lung cancer cells. PMID:24376841
RBP2 may link chronic inflammation to tumor development. PMID:25015565
Epigenetic changes mediated by JARID1A, SMYD3 and DNA methylation may be responsible, at least in part, for the functional progesterone withdrawal that precipitates human labour. PMID:24442343
KDM5A and the NuRD complex cooperatively function to control developmentally regulated genes PMID:25190814
ARID is required for RBP2 demethylase activity in cells and that DNA recognition is essential to regulate transcription PMID:18270511
High expression of KDM3B and KDM5A is associated with a better prognosis (no recurrence after mastectomy p=0.005 and response to docetaxel p=0.005)in breast cancer patients. PMID:23266085
In terminally differentiated cells, common KDM5A and E2F4 gene targets were bound by the pRB-related protein p130, a DREAM complex component. PMID:23093672
Jarid1a/b-mediated H3K4 demethylation contributes to silencing of retinoblastoma target genes in senescent cells, suggesting a mechanism by which retinoblastoma triggers gene silencing. PMID:22615382
Evidence was provided that chronic drug exposure generated drug-tolerant cells via epigenetic mechanisms involving molecules such as CD44 and KDM5A. PMID:21935404
JARID1A or a locus in strong linkage disequilibrium with it is a positional candidate for susceptibility to AS. PMID:21562575
that H3K4 tri- and dimethylation play an important role and JARID1A is the histone-demethylating enzyme responsible for removal of this mark PMID:21348942
Mad1 recruits RBP2 to the hTERT promoter that, in turn, demethylates H3-K4, thereby contributing to a stable repression of the hTERT gene in normal or differentiated malignant cells. PMID:19762557
that RBP2-H1 exerts a broad tumor-suppressive function partially mediated by pRb modulation PMID:16645588
Study shows that RBP2 is displaced from Hox genes during embryonic stem cell differentiation correlating with an increase of their di- and trimethylated histone 3 lysine 4 levels and expression. PMID:17320161
RBP2 associates with MRG15 complex to maintain reduced H3K4 methylation at transcribed regions, which may ensure the transcriptional elongation state PMID:17573780
During differentiation, RBP2 exerts inhibitory effects on multiple genes through direct interaction with their promoters. PMID:18722178
fusing an H3K4-trimethylation-binding PHD finger, such as the carboxy-terminal PHD finger of JARID1A, to a common fusion partner NUP98, generated potent oncoproteins that arrested haematopoietic differentiation and induced acute myeloid leukaemia PMID:19430464