IL1R1 Recombinant Monoclonal Antibody

Code: CSB-RA011621MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

P14778

Target Names

IL1R1

Alternative Names

Antigen CD121a antibody; CD121 antigen-like family member A antibody; CD121a antibody; CD121a antigen antibody; D2S1473 antibody; IL 1 antibody; IL 1R 1 antibody; IL 1R alpha antibody; IL 1RT1 antibody; IL-1R-1 antibody; IL-1R-alpha antibody; IL-1RT-1 antibody; IL-1RT1 antibody; IL1 inhibitor antibody; IL1 Receptor I antibody; IL1R antibody; IL1R1 antibody; IL1R1_HUMAN antibody; IL1RA antibody; IL1RN antibody; IL1RT1 antibody; Interleukin 1 receptor alpha type 1 antibody; Interleukin 1 receptor antagonist protein (IRAP) antibody; Interleukin 1 receptor type I antibody; Interleukin receptor 1 antibody; Interleukin-1 receptor alpha antibody; Interleukin-1 receptor type 1 antibody; Interleukin-1 receptor type I antibody; p80 antibody

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human IL1R1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG

Clone No.

26F8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:20-1:500

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing NIH/3T3 cells surface stained with CSB-RA011621MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Interleukin-1 receptor type 1 serves as the primary signaling receptor for IL-1α and IL-1β, positioning it at the center of inflammatory cascade initiation and immune response regulation. This receptor exists in both membrane-bound and soluble forms, with the soluble variant acting as a decoy receptor that modulates IL-1 signaling intensity. Understanding IL1R1 expression and function has significant implications for cardiovascular disease research, immunology studies, and stem cell biology, where inflammatory signaling pathways influence differentiation and tissue homeostasis.

This recombinant monoclonal antibody, clone 26F8, offers the reproducibility that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than hybridoma supernatants, researchers benefit from consistent performance across lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations. The antibody undergoes affinity chromatography purification, ensuring high purity for sensitive downstream applications.

Validation studies confirm reliable performance in flow cytometry applications, with recommended dilutions ranging from 1:50 to 1:200. Surface staining of NIH/3T3 cells demonstrates clear positive signal separation from isotype control, with the protocol employing FITC-conjugated secondary detection following standard blocking and incubation steps at 4°C. This mouse IgG2a isotype antibody shows cross-species reactivity with both human and mouse samples, providing flexibility for researchers working across model systems or translational studies.

The unconjugated format allows investigators to select secondary detection systems suited to their specific instrumentation and experimental design. For researchers investigating IL-1 signaling in inflammatory conditions, cardiovascular pathology, or stem cell microenvironments, this antibody provides a sequence-defined tool for characterizing receptor expression patterns across relevant cell populations.

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Target Background

Function(From Uniprot)

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.

Gene References into Functions

interleukin-1 receptor 1/MyD88 signalling has roles in the development and progression of pulmonary hypertension PMID:27418552
Study highlights the association of genetic polymorphisms of IL1R1 with knee osteoarthritis susceptibility in the Northwestern Chinese Han population. PMID:27980229
Serum ST2 is elevated in heart failure patients and correlated with disease severity. PMID:28164491
Since mumps virus SH coimmunoprecipitated with tumor necrosis factor receptor 1 (TNFR1), RIP1, and IRAK1, we hypothesize that SH exerts its NF-kappaB activation inhibitory function by interacting with TNFR1, interleukin-1 receptor type 1 (IL-1R1), and TLR3 complexes in the plasma membrane of infected cells. PMID:28659487
salt-inducible kinase inhibition decreases proinflammatory cytokines in human myeloid cells upon IL-1R stimulation. PMID:26590148
study confirmed that genetic variant in IL-1R1(rs3917267) has significant association with HBV infection and HBV breakthrough infection in children, which provides new clues for the study of pathogenesis of chronic HBV infection in children PMID:28027994
The results present identification of critical regions within the TIR domain of IL-1 receptor type in humans and mice. PMID:26279140
IL1R1 SNP rs949963 is associated with the susceptibility to asthma in children from Central China and may increase the serum expression of IL1R1. PMID:26975823
investigated the association between four inflammatory cytokines (CD121a, interleukin [IL]-1beta, IL-8, and IL-11) and coronary heart disease PMID:26098632
This study found that the mRNA expression of IL-1R1, TNFR1, and TNFR2 was significantly higher in schizophreina PMID:25749018
IL-1R1 expression appears to define a tissue regulatory T cell phenotype together with the expression of CD25, glucocorticoid-induced tumor necrosis factor receptor family-related gene and CTLA-4. PMID:26076982
TNF-alpha (-308) GG, IL-10 (-819) TT, IL-10 (-1082) GG and IL1R (+1970) CC genotypes are found to be predominant (p=0.01, p=0.02, p=0.0001 and p=0.001, respectively) in both tuberculoid as well as lepromatous leprosy patients. PMID:25697140
IL-1 receptor gene polymorphisms could be one of the factors influencing the expression of membrane-bound IL-1 receptors on immunocompetent cells. PMID:24976267
severe forms of pancreatitis are associated with increased levels of IL-6, IL-10 and IL-1ra PMID:25240697
SUMOylation is a novel mechanism in the regulation of beta-arrestin 2-mediated IL-1R/TRAF6 signaling PMID:25425640
genetic polymorphism is associated with atopic dermatitis in Iranian pediatric population PMID:23253688
Blood monocytes from renal cell carcinoma patients displayed a tumor-promoting transcriptional profile that supported functions like angiogenesis and invasion. Induction of this protumor phenotype required an interleukin-1 receptor (IL-1R)-dependent mechanism. PMID:25453823
the NLRP3 inflammasome/IL-1RI axis is dispensable for PM10-facilitated allergic sensitization. PMID:24988285
Thirty-eight single nucleotide polymorphisms (SNPs) were identified within genomic regions containing all exons and relevant exon-intron boundaries in IL1R1 and IL1R2 in japanese aggressive periodontitis. PMID:24818754
Only the IL1RN tandem repeats polymorphism may be associated with Hashimoto's thyroiditis susceptibility; TSHR and IL1RN polymorphisms may represent prognostic factors for predicting the severity of the disease. PMID:24328419
). Findings provide evidence of subgroups of women with breast cancer who report distinct trajectories of attentional function and of a genetic association between subgroup membership and an IL1R1 promoter polymorphism. PMID:24315345
No significant differences are detected in the Fcgamma-RIIa and IL1-Ra polymorphisms compared to controls in a study to search for associations between antineutrophil cytoplasm antibody-associated vasculitis and IL1R1 polymorphisms. PMID:24356554
results demonstrate clear functional consequences of the rs917997 risk polymorphism; this polymorphism leads to a loss-of-function through decreased IL-18RAP, IL-18R1, and IL-1R1 protein expression, which impairs autocrine IL-18 and IL-1 signaling PMID:24842757
The frequency of the IL-1RN rs315952 CT genotype was significantly lower among patients with systemic lupus erythematosus compared with healthy controls PMID:23722873
Common IL-33 and IL1RL1 polymorphisms contribute to the risk of inflammatory bowel disease PMID:23634226
The IL1R1 promoter SNP rs2192752 may contribute to the location of papillary thyroid carcinoma (PTC), and the C allele of rs2192752 may be a risk factor for the development of PTC in both lobes. PMID:22594576
These results demonstrate that IL-1alpha and IL-1R1 signaling is essential for microabscess formation, neutrophil recruiting chemokine expression, and acanthosis in psoriasis-like skin inflammation induced by imiquimod. PMID:23407395
The internalization of IL1RI after IL-1 binding is not a mechanism of feedback inhibition but of critical importance for the IL-1-induced gene expression. PMID:23022958
Analysis of the data suggested that IL-1R1 expression in the neonatal period provides an additional level of Myd88-dependent signaling during this period of heighted susceptibility to infection. PMID:22435759
The IL-1R1 1498T>C polymorphism is associated with early-stage endometriosis in Korean women. PMID:22509941
a genetic link between rs1921622 IL1RL1 polymorphism and disease severity in RSV bronchiolitis PMID:22574108
This study demonistrated that Carriers of the minor allele for a single nucleotide polymorphism in interleukin (IL)1-receptor 1 (IL1R1) (rs2110726) were less likely to report breast pain prior to surgery PMID:22515947
the LCAP related changes in sIL-1RI and IL-1Ra might impact the clinical outcome during treatment with an LCAP filter in patients with inflammatory bowel disease PMID:22267087
No sufficient evidence was available to support any associations between IL1-Ra and IL6-147G/C polymorphisms and ischemic stroke. PMID:22417897
a role for TILRR in selective amplification of NF-kappaB responses through IL-1RI and suggest that the specificity is determined by changes in receptor conformation and adapter protein recruitment. PMID:22262840
The study presents the crystal structure of IL-1beta bound to its primary receptor IL-1RI and its receptor accessory protein IL-1RAcP. PMID:22426547
data demonstrated that IL-1R polymorphism is associated with susceptibility to ankylosing spondylitis PMID:22285486
regulates S100A8/A9-dependent keratinocyte resistance to bacterial invasion PMID:22031183
findings demonstrate that neutrophils express IL-1R1 and that IL-1R1 is induced following extravasation and exists in a mobile intracellular compartment PMID:22385245
Data sshow that variants in SFTPD, CD46 and IL1R1 are associated with IPD in both EA and AA. PMID:21858107
IL-1RI expression in regulatory T (Treg)cells identifies a subpopulation at an early stage of differentiation. PMID:21998454
This study found for the first time an epigallocatechin gallate-induced downregulation of the IL-1RI expression possibly being caused by NF-kappaB inhibition. PMID:21787753
IL-1RI expression was not altered in endometrioid carcinoma cells in comparison with endometrial cells, clear-cells, serous and mucinous ovarian cancer cells. PMID:21083841
Interleukin-1 receptor-mediated inflammation impairs the heat shock response of human mesothelial cells PMID:21435443
No association was found between recurrent spontaneous abortion and gene polymorphisms in the interleukin-1 receptor 1 gene. PMID:20956022
IL1RL1 polymorphisms are associated with serum IL1RL1-a, blood eosinophils, and asthma in childhood PMID:21281963
Serum levels of ECP, IL-5, -6, -8 and -10, G-CSF, MCP-1, IL-1 ra and IP-10 were significantly elevated in acute compared with stable childhhood asthma. PMID:20523065
This study indicated that statistically significant higher protein and mRNA levels of the il1r in frontal corte in bipolar disorder. PMID:19488045
findings that human Tregs preferentially express receptors for TNF and IL-1 suggest a potential function in sensing and dampening local inflammation PMID:20066156
An association between severe hand osteoarthritis and IL1R1 gene. PMID:20353565