HAT1 Recombinant Monoclonal Antibody

Code: CSB-RA172317A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC, FC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

O14929

Target Names

HAT1

Alternative Names

HAT 1 antibody; hat1 antibody; HAT1_HUMAN antibody; Histidine aminotransferase 1 antibody; Histone acetyltransferase 1 antibody; Histone acetyltransferase 1 antibody; Histone acetyltransferase type B catalytic subunit antibody; KAT1 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human HAT1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4A10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Jurkat whole cell lysate(30µg), HEK293 whole cell lysate(30µg), A-431 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), HeLa whole cell lysate(30µg), PC-3 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), THP-1 whole cell lysate(30µg)
All lanes: KAT1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3min
IHC image of CSB-RA172317A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing HepG2 cells stained with CSB-RA172317A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

HAT1, also known as histone acetyltransferase 1 or KAT1, serves as the catalytic subunit of the type B histone acetyltransferase complex, playing a fundamental role in chromatin assembly and epigenetic regulation. This enzyme acetylates newly synthesized histone H4 at lysines 5 and 12, a modification essential for proper histone deposition onto nascent DNA during replication. Given its involvement in cell cycle progression and chromatin dynamics, HAT1 has become an important target for researchers investigating epigenetic mechanisms, cancer biology, and cellular proliferation.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human HAT1, offers the consistency and reliability that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability that can compromise longitudinal studies, ensuring reproducible results across experiments conducted months or years apart.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis detects a clean band at the predicted 50 kDa molecular weight across an extensive panel of human cell lines including Jurkat, HEK293, A-431, MCF-7, HeLa, PC-3, HepG2, and THP-1, as well as mouse NIH/3T3 cells, confirming cross-species reactivity with both human and mouse samples. Immunohistochemistry staining in paraffin-embedded human stomach tissue reveals specific nuclear localization consistent with HAT1's chromatin-associated function. Flow cytometry analysis in HepG2 cells shows clear positive signal separation from isotype controls, enabling quantitative assessment of HAT1 expression at the single-cell level.

This antibody supports research into histone modification pathways, DNA replication-coupled chromatin assembly, and epigenetic dysregulation in disease states.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair. Coordinates histone production and acetylation via H4 promoter binding. Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression. Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks. In addition, acetylates other substrates such as chromatin-related proteins. Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation.; (Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.

Gene References into Functions

The expression of HAT1 and MYST1, did not differ between drug-sensitive and -resistant cells PMID:18458078
Human histone acetyltransferase 1 (Hat1) acetylates lysine 5 of histone H2A in vivo. PMID:24682716
The high-resolution crystal structure of a Hat1p/Hat2p/CoA/H4 peptide complex was solved and it was found that the H4 tail interacts with both Hat1p and Hat2p, by which substrate recruitment is facilitated. It was discovered that H3 N-terminal peptides can bind to the Hat2p WD40 domain. PMID:24835250
Signalling from Toll-like or TNF-alpha receptors triggers the calcium/calmodulin-dependent protein kinase (CaMK2) to activate histone acetyltransferase-1 (HAT1), which then acetylates the transcriptional regulator PLZF. Acetylation of PLZF promotes the assembly of a repressor complex incorporating HDAC3 and the NF-kappa B p50 subunit that limits the NF-kappa B response. PMID:25865065
HAT1 is involved in the STAT5 binding to the Bcl2L12 promoter. HAT1 increased the expression of Bcl2L12. Bcl2L12 mediated the effects of HAT1 on suppressing NPC cell apoptosis. Absorption of the HAT1 shRNA plasmid-carrying liposomes induced NPC cell apoptosis. In conclusion, inhibition of HAT1 can induce NPC cell apoptosis via increasing Bcl2L12 expression, which can be a potential therapy for NPC treatment. PMID:29621521
HAT1 was upregulated in hepatocellular carcinoma.HAT1 acts as an oncogenic protein promoting cell proliferation and inducing cisplatin resistance in hepatocellular carcinoma. PMID:27938492
Nuclear HDAC2 immunopositivity was significantly higher in actinic cheilitis (AC) when compared with lip squamous cell carcinoma (LSCC). HDAC1 and HAT1 nuclear immunostaining were higher in AC, with no statistical significance. When comparing data with our previous study, we found a positive correlation between HDAC1 X DNMT1/DNMT3b, HDAC2 X DNMT3b, and HAT1 X DNMT1/DNMT3b for certain studied groups. PMID:28107582
AMPK phosphorylates DNMT1, RBBP7, and HAT1 and increases interactions of DNMT1, RBBP7, and HAT1. PMID:28143904
Results show a novel interaction of the Lsm4 RGG domain with HAT1 and RBBP7, leading to the possibility of a posttranslational modifications network involved in mRNP regulation. PMID:27247266
These data indicated that miR-486 aggravate the cholesterol accumulation in THP-1 cells by targeting HAT1. PMID:26654953
The expression of HAT1 was higher in the primary Esophageal carcinoma tumors and adjacent tissue as compared to that of the normal esophageal tissue and HAT1 expression was directly correlated with the poor tumor differentiation PMID:25120766
Data indicate that HAT1 is required for homologous recombination in DNA repair. PMID:23653357
the role of Hat1 in DNA damage repair is evolutionarily conserved, the ability of H3 acetylation to compensate for Hat1 deletion appears to be more variable PMID:17052979
HAT gene expression is required for cisplatin resistance and Clock and Tip60 regulate not only transcription, but also DNA repair, through periodic histone acetylation PMID:18458078