GOLGA2 Recombinant Monoclonal Antibody

Code: CSB-RA961114A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Target Background

Product Details

Uniprot NO.

Q08379

Target Names

GOLGA2

Alternative Names

Golgin subfamily A member 2 (130 kDa cis-Golgi matrix protein) (GM130) (GM130 autoantigen) (Golgin-95), GOLGA2

Species Reactivity

Human

Immunogen

A synthesized peptide from human GOLGA2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3B11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: U-87MG whole cell lysate(30µg), HepG2 whole cell lysate(30µg)
All lanes: Golgin subfamily A member 2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 113 kDa
Observed band size: 140 kDa
Exposure time：3min
IHC image of CSB-RA961114A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA961114A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing HepG2 cells stained with CSB-RA961114A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

GOLGA2, commonly known as GM130, serves as a critical structural component of the cis-Golgi matrix where it orchestrates vesicle tethering, Golgi ribbon maintenance, and protein trafficking. This peripheral membrane protein has emerged as an important marker in studies of Golgi organization, cell polarity, and secretory pathway dynamics, with growing interest in its altered expression patterns across various disease states including cancer.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human GOLGA2, offers the reproducibility and consistency that demanding research protocols require. As a sequence-defined reagent produced from a single rabbit clone (3B11), it eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations, ensuring your results remain comparable across experiments.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis in U-87MG glioblastoma and HepG2 hepatocellular carcinoma cell lysates reveals robust detection at working dilutions between 1:500 and 1:2000. The observed band at approximately 140 kDa runs higher than the predicted 113 kDa molecular weight, a shift commonly attributed to post-translational modifications including extensive glycosylation characteristic of Golgi-resident proteins. Immunohistochemistry validation in paraffin-embedded human breast cancer and kidney tissue sections confirms reliable performance in archival specimens, while flow cytometry analysis in HepG2 cells demonstrates clear population separation for intracellular detection applications.

Whether investigating Golgi dynamics in cancer cell biology, examining secretory pathway disruption, or characterizing cellular stress responses, this antibody provides a dependable tool for researchers studying membrane trafficking and organelle organization.

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Target Background

Function(From Uniprot)

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization. Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle. Regulates the meiotic spindle pole assembly, probably via the same mechanism. Also regulates the centrosome organization. Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins.

Gene References into Functions

In situ proximity ligation assays of Golgi localization of alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and absence or presence of N-glycans terminated with alpha3-mannose on trans-Golgi glycosyltransferases may be useful for distinguishing indolent from aggressive prostate cancer cells. PMID:28782625
GM130 upregulated expression of the key epithelial-mesenchymal transformation regulator Snail (SNAI1), which mediated EMT activation and cell invasion by GM130. PMID:26617790
the study identified GM130 as a novel target of Coxsackievirus B3 (CVB3), which may implicate in the pathogenesis of CVB3-induced acute pancreatitis. PMID:26314804
We describe the first human patient with a homozygous apparently loss of function mutation in GOLGA2. The phenotype is a neuromuscular disorder characterized by developmental delay, seizures, progressive microcephaly, and muscular dystrophy. PMID:26742501
Data show that WAC directly binds to GM130 and that this binding is required for autophagosome formation through interacting with GABARAP regulating its subcellular localization. PMID:26687599
Mutagenesis experiments support these structural observations and demonstrate that they are required for GRASP65-GM130 association. PMID:26363069
Depletion of GM130 increases cellular velocity and increases the invasiveness of breast cancer cells, therefore supporting the view that alterations of polarity contribute to tumor progression. PMID:25892554
GM130 is a parallel homotetramer with a flexible rod-like structure with I- and Y-shaped conformations. PMID:25787021
H-ERG trafficking was impaired by H2O2 after 48 h treatment, accompanied by reciprocal changes of expression between miR-17-5p seed miRNAs and several chaperones (Hsp70, Hsc70, CANX, and Golga20) PMID:24386440
Induction of autophagy by shGOLGA2 may induce cell death rather than cell survival. Downregulation of GOLGA2/GM130 may be a potential therapeutic option for lung cancer. PMID:22735382
GM130 is involved in the control of glycosylation, cell cycle progression, cell polarization and directed cell migration, according to this review. PMID:20197635
FXIII-A associated with podosomes & other structures adjacent to the plasma membrane, containing TGN46 & GM130 but not protein disulphide isomerase. FXIII-A was present in GM130-positive intracellular vesicles that could mediate its transport. PMID:20086247
the cytoplasmic C terminus of HERG participates in the tethering or possibly targeting of HERG-containing vesicles within the Golgi via its interaction with GM130 PMID:12270925
Mammalian Ste20 kinases YSK1 and MST4 target to the Golgi apparatus via the Golgi matrix protein GM130. PMID:15037601
Ribbon formation requires the Golgi proteins GM130 and GRASP65. PMID:16489344
This is the first report of a role for a Golgi apparatus protein in the regulation of centrosomes during interphase. PMID:18045989
depletion of GM130 by RNA interference slows the rate of ER to Golgi trafficking in vivo; interactions of GM130 with syntaxin 5 and Rab1 are regulated by mitotic phosphorylation PMID:18167358
Cdc42 has a novel role in controlling centrosome organization in unstimulated cells in addition to its known function as a regulator of centrosome reorientation in stimulated cells. PMID:19109421
Data suggest that recruitment of AKAP450 on Golgi membranes through GM130 allows centrosome-associated nucleating activity to extend to the Golgi, to control the assembly of subsets of microtubules. PMID:19242490

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Subcellular Location

Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum-Golgi intermediate compartment membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, spindle pole.

Protein Families

GOLGA2 family

Database Links

HGNC: 4425

UNIGENE: Hs.155827

KEGG: hsa:2801

STRING: 9606.ENSP00000416097

OMIM: 602580

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