FYB Recombinant Monoclonal Antibody

Code: CSB-RA214399A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O15117

Target Names

FYB

Alternative Names

ADAP antibody; Adhesion and degranulation promoting adaptor protein antibody; FYB 120/130 antibody; Fyb antibody; FYB-120/130 antibody; FYB_HUMAN antibody; FYN binding protein antibody; FYN T binding protein antibody; FYN-binding protein antibody; FYN-T-binding protein antibody; p120/p130 antibody; PRO0823 antibody; SLAP 130 antibody; SLAP-130 antibody; SLAP130 antibody; SLP 76 associated phosphoprotein antibody; SLP-76-associated phosphoprotein antibody; SLP76 associated phosphoprotein antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human FYB protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6D11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: A549 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), COLO205 whole cell lysate(30µg)
All lanes: FYN binding protein 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 85 kDa
Observed band size: 120 kDa
Exposure time：2min
IHC image of CSB-RA214399A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing K562 cells stained with CSB-RA214399A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

FYB, also known as ADAP (Adhesion and Degranulation Promoting Adaptor Protein), serves as a critical scaffolding molecule in immune cell signaling cascades. This adaptor protein interacts with FYN kinase and SLP-76, positioning it at the intersection of T cell receptor signaling and integrin-mediated adhesion pathways. Researchers investigating lymphocyte activation, immune synapse formation, and signal transduction mechanisms will find FYB an essential target for understanding how immune cells coordinate their responses.

This recombinant monoclonal antibody, clone 6D11, offers the reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human FYB protein, the recombinant format ensures sequence-defined consistency across lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. Affinity chromatography purification delivers a clean preparation suitable for sensitive detection methods.

Validation across multiple platforms demonstrates this antibody's experimental versatility. Western blot analysis detects FYB in human cell lines including A549, THP-1, and COLO205 whole cell lysates, with an observed band at approximately 120 kDa. This migration above the predicted 85 kDa molecular weight reflects the extensive post-translational modifications, particularly phosphorylation events, characteristic of this heavily regulated adaptor protein. Immunohistochemistry staining in paraffin-embedded human lymph node tissue confirms utility for tissue-based studies, while flow cytometry analysis of K562 cells demonstrates clear positive signal separation, supporting intracellular detection applications.

Whether you are mapping phosphorylation-dependent protein interactions, characterizing immune cell populations, or investigating adhesion signaling networks, this antibody provides validated performance across complementary detection platforms to support comprehensive signal transduction research.

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Target Background

Function(From Uniprot)

Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton. Modulates the expression of IL2. Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.

Gene References into Functions

results of this study indicate that a novel T cell adaptor protein, activation-dependent, raft-recruited ADAP-like phosphoprotein (ARAP), plays a unique role in T cells as a part of both the proximal activation signaling and inside-out signaling pathways that result in integrin activation and T cell adhesion PMID:27335501
Ubc9 is an essential regulator of ADAP where it is required for TCR-induced membrane recruitment of the small GTPase Rap1 and its effector protein RapL and for activation of the small GTPase Rac1 in T cell adhesion. PMID:29127148
Current knowledge of the functions of the adapter protein ADAP in T cell signaling with a focus on the role of individual phosphotyrosine (pY) motifs for SH2 domain mediated interactions is presented. PMID:27258783
A distinct set of proteins interaction partners required for chemokine-directed T cell migration is attracted by phosphotyrosine 571 of ADAP, including ZAP70. PMID:26246585
FYB nonsense mutations in humans causing small-platelet thrombocytopenia and a significant bleeding tendency. PMID:25876182
Data (including data from studies in knockout/transgenic mice) suggest that ADAP regulates positive feedback loop of TGFbeta1 production and TGFbeta1-induced CD103 expression in CD8+ T-lymphocytes and protects against influenza H5N1 virus infection. PMID:25909459
The aim of this study was to perform an association study between seven Fyn-binding protein gene (FYB)-tag single nucleotide polymorphisms (SNPs) and type I diabetes mellitus (T1DM), as well as with disease age of onset. PMID:25729932
The autosomal recessive bleeding phenotype seen in several members of this highly consanguineous family included petechial rash, mild epistaxis and thrombocytopenia with some decrease in platelet volume. These clinical findings, together with the results of exome sequencing pointed to only one strong candidate gene, the FYB gene. PMID:25516138
ADAP interacts with talin and kindlin-3 to promote platelet Integrin alphaIIbbeta3 activation and stable fibrinogen binding. PMID:24523237
ADAP and Nck adapter proteins cooperatively facilitate T cell adhesion to the LFA-1 ligand ICAM-1. PMID:24769494
These findings indicate that ADAP regulates two steps of HIV-1 infection cooperatively with two distinct receptors, and as such, serves as a new potential target in the blockade of HIV-1 infection. PMID:24047317
our findings indicate an association between polymorphisms located in FYB gene and SLE, suggesting their possible involvement in disease susceptibility and clinical manifestations. PMID:23628395
Multipoint binding of SLP-76 to ADAP facilitates the assembly of SLP-76 microclusters. PMID:23979596
TM4SF10, possibly through ADAP, may regulate Fyn activity PMID:21881001
a functional cooperation between Nck and ADAP in stabilizing the recruitment of WASp to SLP76 regulates actin rearrangement. PMID:21536650
the ADAP CARMA1 binding site is required for IKK gamma ubiquitination; both TAK1 and CARMA1 binding sites are required for IkappaB alpha phosphorylation and degradation and NF-kappaB nuclear translocation PMID:20164171
Mass spectrometric identification of ADAP associated with EVL, an actin-binding protein of the ENA/VASP family, DOCK2 and GEF-H1 suggests a direct link between ADAP and the cytoskeleton. PMID:19798671
ADAP lipid interaction defines the helically extended SH3 scaffold as a novel member of membrane interaction domains PMID:15843031
show in an ADAP-deficient Jurkat T cell line that the co-dependence of ADAP and SKAP55 extends beyond their functional and physical interactions and show that SKAP55 protein is unstable in the absence of ADAP PMID:15849195
Lipid binding of ADAP at the immunological synapse most likely contributes to the function of ADAP as a regulator of T cell migration and adhesion. PMID:16831444
An eight-membered ring formed upon oxidation of two neighboring cysteines leads to significant changes in the variable arginine-threonine (RT) loop of the hSH3N domain of this protein. PMID:17511475
ADAP-deficient T-cell-receptor transgenic T cells demonstrate that ADAP is a positive regulator of antigen-dependent, LFA-1 integrin-mediated T cell conjugate formation with antigen presenting cells. PMID:17785790
Naive transgenic ADAP-deficient T cells show impaired adhesion to ovalbumin fragment-bearing antigen-presenting cells that is restored following reconstitution with wild-type ADAP. PMID:18802088