EPHB3 Recombinant Monoclonal Antibody

Code: CSB-RA122093A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P54753

Target Names

EPHB3

Alternative Names

Cek10 antibody; EK2 antibody; Embryonic kinase 2 antibody; EPH Like Tyrosine Kinase 2 antibody; EPH receptor B3 antibody; EPH-like kinase 2 antibody; ephb3 antibody; EPHB3_HUMAN antibody; Ephrin receptor EphB3 antibody; Ephrin type B receptor 3 antibody; Ephrin type-B receptor 3 antibody; ETK2 antibody; hEK2 antibody; Human Embryo Kinase 2 antibody; Mdk5 antibody; Sek4 antibody; TYRO6 antibody; Tyrosine protein kinase receptor HEK2 antibody; Tyrosine protein kinase TYRO6 antibody; Tyrosine-protein kinase TYRO6 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human EPHB3 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2H5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
, Positive WB detected in:A431 whole cell lysate(30µg), A375 whole cell lysate(30µg), U251 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg)
All lanes: EPHB3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 110 kDa
Observed band size: 130 kDa
Exposure time：180s
IHC image of CSB-RA122093A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF-7 cells stained with CSB-RA122093A0HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Description

EPHB3 belongs to the Eph receptor tyrosine kinase family, the largest subfamily of receptor tyrosine kinases, playing essential roles in axon guidance, cell migration, and tissue boundary formation during neural development. This receptor's interactions with ephrin-B ligands mediate bidirectional signaling that influences synaptic plasticity and neuronal connectivity, making it a compelling target for neuroscience research exploring developmental processes and neurological disorders.

This recombinant monoclonal antibody, generated from clone 2H5 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis detects EPHB3 in several human cell lines including A431, A375, U251, and SH-SY5Y at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 130 kDa runs slightly higher than the predicted 110 kDa molecular weight, consistent with post-translational modifications such as glycosylation that are characteristic of receptor tyrosine kinases. Immunohistochemistry on paraffin-embedded human skin tissue shows clear staining using citrate buffer antigen retrieval, while flow cytometry analysis of MCF-7 cells confirms utility for single-cell protein expression studies.

The antibody's affinity-purified format and compatibility with ELISA, western blot, immunohistochemistry, and flow cytometry provides flexibility for researchers investigating EPHB3 biology across different experimental contexts, from expression profiling in neural cell models to tissue localization studies.

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Target Background

Function(From Uniprot)

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Gene References into Functions

These results indicated the suppressive effect of Form on colon carcinoma cell proliferation and invasion, possibly via miR149induced EphB3 downregulation and the inhibition of the PI3K/AKT and STAT3 signaling pathways. PMID:29620230
work suggested that EphB3 acted as a tumor promoter in Papillary Thyroid Cancer by increasing the in vitro migration as well as the in vivo metastasis of Papillary Thyroid Cancer cells through regulating the activities of Vav2 and Rho GTPases in a kinase-dependent manner. PMID:27986811
These results show that EphB3 protein is lost in ovarian serous carcinoma and is associated with tumor grade and FIGO stage, which indicate that EphB3 protein may play a role in carcinogenesis of ovarian serous carcinoma and may be used as a molecular marker for prognosis. PMID:28120491
results identify EPHB3 as a novel target of SNAIL1 and suggest that disabling EPHB3 signaling is an important aspect to eliminate a roadblock at the onset of EMT processes. PMID:25277775
These results uncover enhancer decommissioning as a mechanism for transcriptional silencing of the EPHB3 tumor suppressor. PMID:24707046
EphB3 suppresses non-small-cell lung cancer metastasis via a PP2A/RACK1/Akt signalling complex PMID:22314363
Our work shows that EphB3 is consistently expressed by malignant T lymphocytes, most frequently in combination with EphB6, and that stimulation with their common ligands strongly suppresses Fas-induced apoptosis in these cells. PMID:22039307
EphB3 provides critical support to the development and progression of NSCLC by stimulating cell growth, migration, and survival. PMID:21266352
Ephrin B3 receptor regulates the synthesis and release of D-serine in astrocytes, which may have important implications on synaptic transmission and plasticity. PMID:21106840
while catalytic activity of EphB3 is required for inhibition of integrin-mediated cell adhesion, a distinct signaling pathway to Rho GTPases shared by WT- and KD-EphB3 receptor mediates inhibition of directional cell migration PMID:15536074
EphB3-ephrin-B interaction promotes mesenchymal-to-epithelial transition (MET) by re-establishing epithelial cell-cell junctions and such an MET-promoting effect contributes to EphB3-mediated tumor suppression. PMID:19483190
EPHB3, MASP1 and SST map to 3q26.2-q29 and may have roles in squamous cell carcinoma of the lung PMID:19607727