ENPEP Recombinant Monoclonal Antibody

Code: CSB-RA552900A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

Q07075

Target Names

ENPEP

Alternative Names

Glutamyl aminopeptidase (EAP) (EC 3.4.11.7) (Aminopeptidase A) (AP-A) (Differentiation antigen gp160) (CD antigen CD249), ENPEP

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Aminopeptidase A / CD249

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2G11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA552900A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Description

Glutamyl aminopeptidase, also known as aminopeptidase A or CD249, plays a critical role in the renin-angiotensin system by cleaving angiotensin II to generate angiotensin III, positioning it as a key regulator of blood pressure and fluid homeostasis. Beyond its enzymatic function, ENPEP serves as a differentiation antigen (gp160) expressed on vascular endothelial cells and renal proximal tubule epithelium, making it a valuable marker for studies in immunology and stem cell biology where lineage-specific identification is essential.

This recombinant monoclonal antibody, clone 2G11, offers the reproducibility and consistency that demanding research protocols require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures experimental comparability across collaborating laboratories. The rabbit host origin provides high affinity binding characteristics, while affinity chromatography purification delivers a preparation optimized for sensitive detection applications.

Validation in immunohistochemistry demonstrates reliable performance in paraffin-embedded human kidney tissue, where ENPEP is abundantly expressed. Using a citrate buffer antigen retrieval protocol at pH 6.0 and working dilutions between 1:50 and 1:200, the antibody produces clear visualization of target protein distribution when detected with HRP-conjugated secondary systems and DAB chromogen. This validated IHC protocol, along with confirmed ELISA compatibility, provides researchers with flexibility across experimental platforms.

For investigators exploring renal physiology, cardiovascular regulation, or stem cell differentiation pathways, this antibody delivers the technical reliability needed to confidently characterize ENPEP expression in human tissue specimens.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.

Gene References into Functions

We suggest that APA enzymatic activity affects tumor initiation and cancer malignancy in a TWIST-dependent manner PMID:28177885
The substrate Angiotensin II, the enzymes aminopeptidases-A, B, M as well as IRAP were detected in the jejunal mucosa. PMID:26311161
This study suggests a role for GAP in the neoplastic development of renal tumours and provides additional data for considering the activity and expression of this enzyme of interest in the diagnosis and prognosis of renal neoplasms. PMID:24885240
These results explain the calcium-modulated substrate specificity of APA in central hypertension regulation PMID:23888046
There was no significant alteration in plasma APA activity in the patients with Chagas disease or dilated cardiomyopathies, as compared with that in health controls PMID:21697726
Rieger syndrome with microdeletions including only the PITX2 and ENPEP (glutamyl aminopeptidase) genes. PMID:17850355
Data show that APA appears to be an essential enzyme in the control of blood pressure via degradation of Angiotensin II. PMID:17990103
Data show that aminopeptidase A (APA) plays important roles in the regulation of blood pressure under both the physiological and pathological conditions. PMID:17999179
Data show that brain aminopeptidases act upon Ang I, Ang II and Ang III in order to activate brain AT(1) receptors. PMID:18188697
rare and common variation in ENPEP may contribute to the development of renal and hypertensive disorders and warrants further study PMID:18206321
The presence of APA in several human brain nuclei sensitive to angiotensins and involved in blood pressure regulation suggests that APA in humans is an integral component of the brain renin angiotensin system. PMID:18410507
A novel nonsense polymorphism in the aminopeptidase-a gene was associated with hypertension among postmenopausal women, and those with marginal calcium and vitamin D intake. PMID:18550936
Angiotensin III and aminopeptidase A and N, related converting enzymes, contribute to cell proliferation of prostate cancer and may be implicated in cancer progression. PMID:18677709
Over-expressed APA drastically reduces, in a calcium dependent manner, full-length-Abeta but not amyloid-beta precursor protein (APP) intracellular domain in a cell-free model of Abeta production. PMID:19187443
The activity of glutamyl aminopeptidase was significally increased in head and neck squamous cell carcinoma tissue. PMID:19373777

Hide All

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Protein Families

Peptidase M1 family

Tissue Specificity

Expressed in choriocarcinoma cancer cell lines (at protein level). Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.

Database Links

HGNC: 3355

UNIGENE: Hs.435765

KEGG: hsa:2028

STRING: 9606.ENSP00000265162

OMIM: 138297

Promotions