EGLN2 Recombinant Monoclonal Antibody

Code: CSB-RA088784A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, IF, FC

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Target Background

Product Details

Uniprot NO.

Q96KS0

Target Names

EGLN2

Alternative Names

Egl nine homolog 2 (EC 1.14.11.29) (Estrogen-induced tag 6) (HPH-3) (Hypoxia-inducible factor prolyl hydroxylase 1) (HIF-PH1) (HIF-prolyl hydroxylase 1) (HPH-1) (Prolyl hydroxylase domain-containing protein 1) (PHD1), EGLN2, EIT6

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human EGLN2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HL-60 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Hela whole cell lysate(30µg), Mouse brain tissue lysate(30µg)
All lanes: PHD1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 44 kDa
Observed band size: 55 kDa
Exposure time：2min
Immunofluorescence staining of A549 cell with CSB-RA088784A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing MCF-7 cells stained with CSB-RA088784A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

EGLN2, also known as PHD1 (prolyl hydroxylase domain-containing protein 1), serves as a critical oxygen sensor in cellular physiology. As one of three prolyl hydroxylases that regulate hypoxia-inducible factor (HIF) stability, EGLN2 hydroxylates specific proline residues on HIF-α subunits under normoxic conditions, targeting them for proteasomal degradation. This regulatory mechanism positions EGLN2 at the center of cellular oxygen sensing pathways, making it a compelling target for researchers investigating hypoxia responses, metabolic adaptation, and tumor microenvironment biology.

This recombinant monoclonal antibody, generated against a synthetic peptide from human EGLN2, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant production yields a sequence-defined antibody from a single clone, researchers can expect uniform performance across experiments and between lot numbers, eliminating a common source of variability in long-term studies.

Validation across multiple platforms demonstrates this antibody's experimental flexibility. Western blot analysis confirms specific detection in human cell lines including HL-60, MCF-7, and HeLa, with cross-species reactivity demonstrated in mouse brain tissue. The observed band at approximately 55 kDa runs higher than the predicted 44 kDa molecular weight, likely reflecting post-translational modifications such as glycosylation that are characteristic of the native protein. Immunofluorescence staining in A549 cells reveals clear cytoplasmic localization, while flow cytometry analysis in MCF-7 cells shows distinct positive population shifts compared to isotype controls.

This antibody supports investigations into oxygen-sensing mechanisms, HIF pathway regulation, and the metabolic reprogramming that characterizes cancer progression and ischemic disease models.

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Target Background

Function(From Uniprot)

Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappa-B activation in hypoxic conditions. Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4.

Gene References into Functions

4-bp Insertion/deletion Polymorphism within the Promoter of EGLN2 is associated with breast cancer. PMID:29693343
Our study provided initial evidence that the insertion/deletion polymorphism rs10680577 may play a functional role in the development of CRC in the Chinese population PMID:28218358
In advanced-stage Hodgkin's Lymphoma patients strong cytoplasmic PHD1 expression in Reed-Sternberg cells was associated with poor relapse-free survival among patients treated with involved-field radiotherapy and advanced-stage patients treated with doxorubicin, bleomycin, vinblastine and darcabazine and involved-field radiotherapy. PMID:29277791
This study provides new information relating to the possible mechanism of therapeutic action of hydroxylase inhibitors that has been reported in pre-clinical models of intestinal and hepatic disease. PMID:27130823
PHD1 is phosphorylated by CDK2, CDK4 and CDK6 at Serine 130. PMID:26644182
siRNA-mediated knockdown of PHD1 inhibited glucose-stimulated insulin secretion in pancreatic Beta cells. PMID:26997627
EglN2 associates with the NRF1-PGC1alpha complex and controls mitochondrial function in breast cancer PMID:26492917
rs3733829 in the EGLN2 gene is significantly associated with the risk of COPD in Chinese populations of Hainan province. PMID:25609945
study conducted to investigate the association between gastric cancer (GC) susceptibility with a 4-bp insertion/deletion polymorphism (rs10680577) in the proximal promoter of EGLN2; findings showed that the heterozygote and the homozygote 4-bp del/del confer a significantly increased risk of GC PMID:24517638
Data indicate that the prolyl hydrolase 1 (PHD1) rs10680577 polymorphism is associated with the risk of non-small cell lung cancer in a Chinese population. PMID:24894671
PHD-1 played an important role in hypoxic response pathway of trophoblast through modulating the level of HIF-2alpha. PMID:24644426
PHD1 could induce cell cycle arrest in lung cancer cells, resulting in the suppression of cell proliferation. PMID:24935227
Results show that variants in two adjacent genes, EGLN2 and CYP2A6, influence smoking behavior related to disease risk. PMID:24045616
Onconeuronal antigen Cdr2 correlates with HIF prolyl-4-hydroxylase PHD1 and worse prognosis in renal cell carcinoma. PMID:23531419
Findings provided strong evidence for the hypothesis that rs10680577 contributes to hepatocarcinogenesis, possibly by affecting RERT-lncRNA structure and subsequently EGLN2 expression. PMID:23026137
miR-205 serves a protective role against both oxidative and endoplasmic reticulum stresses via the suppression of EGLN2 and subsequent decrease in intracellular reactive oxygen species. PMID:22859986
Principal component analysis of the covariance matrix of free AIRE-PHD1 highlights the presence of a "flapping" movement, which is blocked in an open conformation upon binding to H3K4me0. PMID:23077531
PHD1 expression correlated with high proliferation, and these tumors were mainly estrogen receptor-negative. PMID:21877141
Coexistence of PHD1 stabilized ATF4, as opposed to the destabilization of ATF4 by PHD3. PMID:21951999
human prolyl hydroxylase might play an important role in determining the physiology and structure of the corpora lutea during the menstrual cycle and early pregnancy PMID:20840881
role for PHD1 as a positive regulator of intestinal epithelial cell apoptosis in the inflamed colon PMID:20600011
Prolyl hydroxylase-dependent (but hypoxia inducible factor HIF-1alpha and -2alpha-independent) activation of hypoxia-induced monocyte-endothelial adhesion assigns a new function to monocytic ICAM-1 under acute hypoxic conditions. PMID:20574001
identified a human homolog of Caenorhabditis elegans Egl9 as a HIF prolyl hydroxylase PMID:12351678
effect of WT or mutated VHL on PHD 1, 2, and 3 PMID:15156561
defines the existence of two species of PHD1 and provide evidence that they are generated by alternative translational initiation PMID:16509823
Understanding the mechanisms by which nickel can inhibit HIF-PHD's and stabilize HIF-1alpha may be important in the treatment of cancer and ischemic diseases. PMID:16649251
Multiple mitochondrial products, including tricarboxylic acid intermediates and reactive oxygen species, can coordinate PHD activity, HIF stabilization, and cellular responses to O(2) depletion. PMID:17101781
hypoxia releases repression of NFkappaB activity through decreased prolyl hydroxylase-dependent hydroxylation of IKKbeta PMID:17114296
Results describe the expression, purification and characterization of the human prolyl hydroxylase PHD1 in Escherichia coli. PMID:18710826
The identification of EGLN2 as a significantly deregulated gene that maps within the paired chromosome region directly implicates defects in the oxygen-sensing network to the biology of renal oncocytoma. PMID:18773095
Overexpression of the oxygen sensor PHD-1 is associated with tumor aggressiveness in pancreatic endocrine tumors. PMID:18927305
Authors identify an interaction between melanoma antigen-11 (MAGE-11) cancer-testis antigen and the major HIF-alpha hydroxylating enzyme PHD2. PMID:19147576
Prolyl hydroxylase inhibitor reduced mitochondrial cytochrome c release, nuclear translocation of apoptosis inducing factor (AIF), and promoted Akt phosphorylation PMID:19229863

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and ca

Database Links

HGNC: 14660

UNIGENE: Hs.515417

KEGG: hsa:112398

STRING: 9606.ENSP00000307080

OMIM: 606424

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