CS Recombinant Monoclonal Antibody

Code: CSB-RA056302A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat

Application: ELISA, WB, IHC, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O75390

Target Names

Alternative Names

Citrate synthase, mitochondrial (EC 2.3.3.1) (Citrate (Si)-synthase), CS

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human CS protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9B9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Hela whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: Citrate synthase antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time：20s
IHC image of CSB-RA056302A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA056302A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela cell with CSB-RA056302A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing PC-3 cells stained with CSB-RA056302A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

Citrate synthase serves as the gateway enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate to form citrate in the mitochondrial matrix. As a key regulator of cellular energy metabolism, CS expression and activity are frequently examined in studies of mitochondrial function, metabolic disorders, oxidative capacity, and exercise physiology. Its consistent expression across tissues also makes it a widely used marker for mitochondrial content and integrity.

This recombinant rabbit monoclonal antibody against human citrate synthase offers the reproducibility that metabolic research demands. Generated from a defined sequence and produced through recombinant technology, it eliminates the lot-to-lot variability inherent to traditional hybridoma-derived antibodies, ensuring your quantitative comparisons remain valid across extended studies.

Validation data demonstrates robust performance across multiple experimental platforms. In western blot applications, the antibody detects a clean band at the predicted 52 kDa molecular weight across diverse human cell lines including SH-SY5Y, HEK293, HeLa, MCF-7, K562, and Jurkat cells, as well as mouse NIH/3T3 cells and rat brain tissue, confirming reliable cross-species reactivity. Immunohistochemistry staining has been validated in human glioma and kidney tissue sections, while immunofluorescence studies in HeLa cells reveal the expected mitochondrial localization pattern. Flow cytometry validation in PC-3 cells demonstrates clear positive population shifts suitable for single-cell metabolic profiling.

This antibody supports researchers investigating mitochondrial biogenesis, cellular respiration, cancer metabolism, neurodegeneration, and exercise-induced adaptations where accurate CS quantification is essential for meaningful biological conclusions.

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Target Background

Gene References into Functions

citrate synthase and ACSS1 have tumorigenic functions in hepatocellular carcinoma PMID:27363021
Citrate synthase activity was lower in patients with heart failure with preserved ejection fraction compared to controls. PMID:27179829
mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID:27793029
METTL12 methylates CS on Lys-395, which is localized in the CS active site. Interestingly, the METTL12-mediated methylation inhibited CS activity and was blocked by the CS substrate oxaloacetate. PMID:28887308
Data suggest that METTL12, localized in mitochondrial matrix, modifies/methylates Lys368 of citrate synthase in external surface region close to its catalytic site; addition/removal of methylation has no effect on citrate synthase activity; Lys368 occurs in highly conserved sequence of amino acid residues of citrate synthase. (METTL12 = methyltransferase-like protein 12) PMID:28391595
Data suggest that downregulation of citrate synthase (CS) expression in 293T cells leads to low level of ATP production, excessive superoxide formation and cell apoptosis, which implies a possible mechanism for hearing loss in A/J mice. PMID:28216161
Hence, we conclude that SIRT3 exhibits neuroprotection via deacetylating and increasing mitochondrial enzyme activities. PMID:28161643
Paretic muscle in hemiparetic stroke survivors had lower CS concentration. PMID:26361074
The citrate synthase knockdown cells exhibited severe defects in respiratory activity and marked decreases in ATP production, but great increases in glycolytic metabolism. PMID:23139858
Citrate synthase (CS) is a direct RORalpha target gene and one mechanism by which RORalpha regulates lipid metabolism is via regulation of CS expression. PMID:22485150
Responses of skeletal and cardiac muscles in CS activity and gene expression at 1 and 48 h after endurance training. Acute effect of exercise on training-induced elevation in CS activity in rat soleus but not ventricle muscles. PMID:12531911
possible role of CS-specific autoantibodies in the pathomechanism of allograft vasculopathy PMID:15711981
It is likely that enhanced citrate synthase activity contributes to the conversion of glucose to lipids in pancreatic cancer providing substrate for membrane lipids synthesis. PMID:15714131
These results provide an important basis for the study of mitochondrial dysfunction due to aberrant CSa trafficking. PMID:19479947