CRP Recombinant Monoclonal Antibody

Code: CSB-RA005991MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P02741

Target Names

CRP

Alternative Names

C-reactive protein [Cleaved into: C-reactive protein(1-205)], CRP, PTX1

Species Reactivity

Human

Immunogen

Recombinant Human CRP protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

23C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA005991MA1HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing HepG2 cells stained with CSB-RA005991MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

C-reactive protein serves as one of the most clinically significant acute-phase reactants in human biology, synthesized primarily by hepatocytes in response to inflammatory cytokines. As a pentameric member of the pentraxin family, CRP plays essential roles in innate immunity through complement activation and opsonization of pathogens. Its dramatic elevation during infection, tissue injury, and chronic inflammatory conditions has established CRP as an indispensable biomarker in both clinical diagnostics and inflammation research.

This recombinant monoclonal antibody, clone 23C11, offers researchers the reproducibility advantages inherent to sequence-defined recombinant production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures consistent performance across lots, eliminating the variability that can compromise longitudinal studies or assay standardization efforts. The human IgG1 isotype format and affinity-chromatography purification yield a reagent suitable for demanding quantitative applications.

Validation studies demonstrate reliable performance across multiple experimental platforms. Immunohistochemistry testing in paraffin-embedded human liver tissue, the primary site of CRP synthesis, confirms effective antigen detection using citrate buffer retrieval at dilutions of 1:50 to 1:200. Flow cytometry analysis using HepG2 hepatocellular carcinoma cells shows clear positive signal separation from isotype control, with fixed and permeabilized cells stained at 1:100 dilution, indicating suitability for intracellular CRP detection in this hepatic cell model. The antibody is additionally validated for ELISA applications.

This antibody supports investigations into inflammatory pathway mechanisms, cardiovascular disease risk assessment, infection biology, and hepatocyte function studies where reliable CRP detection is essential for meaningful experimental outcomes.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Gene References into Functions

gemcabene decreases CRP by C/EBP-delta and NF-kappaB-mediated transcriptional mechanism and suppresses IL-6 and IL-1beta-induced CRP production. PMID:29644527
Combinative index (arterial lactate, NT-proBNP and CRP) could serve as a valuable predictor for the 28-day mortality rate in patients with sepsis. PMID:30158031
CRP rs3093061, rs1130864, rs1205, and rs3091244 polymorphisms are associated with decreased risk of developing Systemic Lupus erythematosus PMID:29556849
The study showed that higher CRP and lower T3 levels were associated with stroke severity on admission. Functional outcome is likely secondary to stroke severity but functional outcome at discharge was associated with higher CRP levels. PMID:30056003
These findings reveal that the CRP- and PTX3-binding characteristics of FHL-1 differ from those of FH, likely underpinning independent immune regulatory functions in the context of the human retina. PMID:29374201
Short-term changes in daily movement behaviour can affect salivary CRP, a marker of systemic inflammation. PMID:29499122
M1 macrophages were characterized by elevated CRP mRNA expression (by 67%), CRP protein levels (by 108%), and upregulation of NFkappaB activation PMID:29687414
Levels of CRP and PCT in CSF did not present greater accuracy in differentiating bacterial from viral meningitis compared to serum levels. PMID:30143494
elevated serum C-reactive protein (CRP) is a well-known proxy for infections/immune abnormalities. Our results show elevated C-reactive protein levels to be associated with an increase in risk for schizophrenia. PMID:29306175
High CRP expression is associated with Nonalcoholic fatty liver disease. PMID:29402657
High C-reactive protein expression is associated with impaired glucose tolerance. PMID:29078256
Findings indicate that childhood infections do not have an independent, lasting effect on circulating inflammatory marker levels subsequently in childhood; however, elevated inflammatory markers may be harmful for intellectual development/function. PMID:29198208
maternal serum level has predictive value for chorioamnionitis PMID:29430983
serum level elevated in preterm labor leading to preterm birth PMID:29426269
Among healthy women attempting pregnancy after one or two pregnancy losses, elevated C-reactive protein is associated with reduced fecundability. PMID:29317123
Suggest a close association between CRP and metabolic syndrome, while the association between the dietary inflammatory index and MetS was limited. PMID:29954070
pre-procedural serum CRP could be a valuable predictor of global cardiovascular risk. PMID:28128312
Data suggest that serum CRP levels show positive association with ChildTurcottePugh scores in subjects with liver cirrhosis; GSH levels show negative association with ChildTurcottePugh scores; thus, CRP and GSH appear to exert synergistic effects in association with severity of liver cirrhosis. This cross-sectional study was conducted in Taiwan. (GSH = glutathione) PMID:29861471
Study shows for the first time that CRP activates signaling of integrin alpha2 via binding to integrin alpha2 and Fcgamma receptor I in breast cells and MDA-MB-231 triple-negative human breast cancer cells. PMID:28846105
This meta-analysis suggests that C-reactive protein/albumin ratio (CAR)may be a potential prognostic marker in solid cancers PMID:28128229
The results of the study suggest that the levels of C-reactive protein and tumor necrosis factor-alpha are important diagnostic markers of inflammation in patients with chronic pancreatitis and type 2 diabetes mellitus. PMID:30280549
Elevated serum C-reactive protein could be a bacterial infection predictor in decompensated cirrhosis. PMID:29384797
The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of Cardiac biomarkers . Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. PMID:29857573
Data suggest that C-reactive protein (CRP) may play an important role in the development of tongue squamous cell carcinoma (TSCC). PMID:29385406
For WBC, N (%), and ESR, both groups showed similar patterns of temporal values 14 days after surgery regardless of preoperative CRP level. Our findings could be used as guidelines for patient discharge and during the follow-up period after surgery. PMID:29854731
The neutrophil/lymphocyte ratio is a better addition to C-reactive protein than CD64 index as a marker for infection in COPD. PMID:28185443
This retrospective cohort study aimed to identify the early postoperative kinetics of C-reactive protein (CRP) and procalcitonin (PCT) in children undergoing tetralogy of Fallot (ToF) correction. PMID:29111402
After beta-blocker treatment, hs-CRP levels are associated with functional capacity in heart failure patients. PMID:29396658
Hypertensive patients with coronary tortuosity have a higher serum hs-CRP level concentration and have a higher incidence of lacunar infarction than hypertensive patients without coronary tortuosity. PMID:29436255
High CRP expression is associated with non-small cell lung cancer. PMID:29549910
T allele of rs1205 in CRP gene was protective against Takayasu arteritis in our patients. CC genotype was associated with involvement of coronary artery and ascending aorta. A trend toward a blunted CRP response seen in patients. PMID:29024426
Serum C-reactive protein (and COMP) levels were both associated with the incidence of knee osteoarthritis. PMID:29351749
coexistence of both higher HCY and higher hs-CRP in the acute phase of ischemic stroke were associated with subsequent PSD, independently of established conventional risk factors PMID:29325799
Data suggest that C-reactive-protein (CRP) concentration is weakly associated with global DNA methylation level, and this association was more clearly in individuals with the minor allele of the 5,10-methylenetetrahydrofolate reductase (MTHFR)missense SNP rs1801133. PMID:29439678
A longitudinal association between adolescent CRP levels and adult schizophrenia diagnosis indicates a potentially important role of inflammation in the pathogenesis of the illness PMID:27622678
rs1205 genetic variability in the CRP gene determines the CRP levels in sepsis of different severities PMID:29379005
CRP levels predict the development of anastomotic leak and postoperative mortality after colorectal cancer surgery. PMID:28132052
Uremia patients receiving maintenance hemodialysis with hospitalacquired infection had increased serum inflammatory factors and high throughput hemodialysis significantly decreased CRP, IL2 and TNFalpha levels in the serum, suggesting that high throughput hemodialysis may be beneficial for the prevention of the infections in uremia patients. PMID:29257244
High CRP expression is associated with cardiovascular disease. PMID:28724840
The results of this meta-analysis suggest that increased CRP expression indicates a poorer prognosis in patients with bone neoplasms. PMID:29668751
C-reactive protein (CRP) recognized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG) in the supported 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers without calcium at pH 7.4 and 5.5. PMID:29172154
Used Ultra Performance Liquid Chromatography-Ultra Definition Mass Spectrometry-based proteomics to compare serum samples from 19 colorectal cancer (CRC) patients. The samples were first analyzed after dividing them into two categories based on CRP, CRP <30 and CRP >30, and the same samples were analyzed again after being divided into the categories short and long 5-year survival. PMID:29630649
CRP bound to surface CD32 (also known as FcgammaRII) on myeloma cells, which activated a pathway mediated by the kinase p38 MAPK and the transcription factor Twist that enhanced the cells' secretion of osteolytic cytokines. PMID:29233917
high-sensitivity C-reactive protein could have a role in recurrent stroke and vascular events in patients treated with statins PMID:28302952
Procalcitonin and CRP serum levels are associated with the appearance of intra-abdominal infection after colorectal cancer surgery. PMID:28918433
C-reactive protein polymorphisms and DNA methylation of the AIM2 gene influence associations between trauma exposure, posttraumatic stress disorder, and C-reactive protein. PMID:28867284
Elevated CRP level is correlated with poor prognosis in patients with metastatic renal cell carcinoma receiving tyrosine kinase inhibitors treatment. (Meta-analysis) PMID:29080691
The study reveals that CRP might be a clear cell renal cell carcinoma development promoter. PMID:28923830
CRP distinguished sepsis and non-septic ICU patients. PMID:28898742
Suggest that hs-CRP may be a marker of comorbidities associated with worse health status in myocardial infarction. PMID:28946036