Human high sensitivity C-Reactive Protein,hs-CRP ELISA Kit

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Human high sensitivity C-Reactive Protein,hs-CRP ELISA Kit

Name: Human high sensitivity C-Reactive Protein,hs-CRP ELISA Kit
Brand: CUSABIO
SKU: CSB-E08617h-IS

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Code: CSB-E08617h-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, cell culture supernates, tissue homogenates

Detect Range: 0.78 ng/mL-50ng/mL

Sensitivity: 0.39 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Alternative Names

Pentraxin 1, short ELISA Kit; C reactive protein ELISA Kit; C reactive protein pentraxin related ELISA Kit; C-reactive protein(1-205) ELISA Kit; CRP ELISA Kit; CRP_HUMAN ELISA Kit; MGC88244 ELISA Kit; Pentraxin 1 ELISA Kit; PTX 1 ELISA Kit; PTX1 ELISA Kit

Abbreviation

hs-CRP

Uniprot NO.

P02741

Species

Homo sapiens (Human)

Sample Type

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.78 ng/mL-50ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human hs-CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:200	Average %	87
	Range %	82-94
1:400	Average %	93
	Range %	89-98
1:800	Average %	99
	Range %	92-106
1:1600	Average %	94
	Range %	89-100

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

3.077

2.934

3.006

2.899

2.399

2.267

2.333

2.226

12.5

1.734

1.728

1.731

1.624

6.25

1.056

1.029

1.043

0.936

3.12

0.726

0.711

0.719

0.612

1.56

0.469

0.453

0.461

0.354

0.78

0.291

0.284

0.288

0.181

0.109

0.105

0.107

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of high-sensitivity C-reactive protein (hs-CRP) in human samples, targeting the low-grade inflammatory concentrations that standard CRP assays cannot resolve. Because hs-CRP levels in the 1–3 mg/L range carry critical prognostic significance for cardiovascular risk stratification, this kit is designed to discriminate within that clinically meaningful low-concentration window.

Detection Range: The 0.78–50 ng/mL range captures hs-CRP concentrations spanning baseline healthy levels through moderate acute-phase elevations. Since circulating hs-CRP in serum typically falls between 0.5–10 mg/L, samples require dilution on the order of 1:100–1:1000, which should be optimized based on the clinical or experimental context.

Sensitivity: At 0.39 ng/mL, the assay detects hs-CRP at concentrations corresponding to the lowest cardiovascular risk tier (<1 mg/L after dilution correction), enabling differentiation between low-risk and moderate-risk individuals that conventional CRP assays miss entirely.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates supports studies linking systemic hs-CRP levels to localized hepatic production or adipose tissue inflammation, connecting circulating biomarker data with tissue-level mechanistic experiments.

Assay Efficiency: The 1–5 hour protocol using 50–100 µL accommodates both high-throughput cohort screening and smaller mechanistic studies where sample volume from primary hepatocyte cultures is limited.

This kit supports research in cardiovascular risk assessment, metabolic syndrome progression, and chronic low-grade inflammation.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Gene References into Functions

gemcabene decreases CRP by C/EBP-delta and NF-kappaB-mediated transcriptional mechanism and suppresses IL-6 and IL-1beta-induced CRP production. PMID:29644527
Combinative index (arterial lactate, NT-proBNP and CRP) could serve as a valuable predictor for the 28-day mortality rate in patients with sepsis. PMID:30158031
CRP rs3093061, rs1130864, rs1205, and rs3091244 polymorphisms are associated with decreased risk of developing Systemic Lupus erythematosus PMID:29556849
The study showed that higher CRP and lower T3 levels were associated with stroke severity on admission. Functional outcome is likely secondary to stroke severity but functional outcome at discharge was associated with higher CRP levels. PMID:30056003
These findings reveal that the CRP- and PTX3-binding characteristics of FHL-1 differ from those of FH, likely underpinning independent immune regulatory functions in the context of the human retina. PMID:29374201
Short-term changes in daily movement behaviour can affect salivary CRP, a marker of systemic inflammation. PMID:29499122
M1 macrophages were characterized by elevated CRP mRNA expression (by 67%), CRP protein levels (by 108%), and upregulation of NFkappaB activation PMID:29687414
Levels of CRP and PCT in CSF did not present greater accuracy in differentiating bacterial from viral meningitis compared to serum levels. PMID:30143494
elevated serum C-reactive protein (CRP) is a well-known proxy for infections/immune abnormalities. Our results show elevated C-reactive protein levels to be associated with an increase in risk for schizophrenia. PMID:29306175
High CRP expression is associated with Nonalcoholic fatty liver disease. PMID:29402657
High C-reactive protein expression is associated with impaired glucose tolerance. PMID:29078256
Findings indicate that childhood infections do not have an independent, lasting effect on circulating inflammatory marker levels subsequently in childhood; however, elevated inflammatory markers may be harmful for intellectual development/function. PMID:29198208
maternal serum level has predictive value for chorioamnionitis PMID:29430983
serum level elevated in preterm labor leading to preterm birth PMID:29426269
Among healthy women attempting pregnancy after one or two pregnancy losses, elevated C-reactive protein is associated with reduced fecundability. PMID:29317123
Suggest a close association between CRP and metabolic syndrome, while the association between the dietary inflammatory index and MetS was limited. PMID:29954070
pre-procedural serum CRP could be a valuable predictor of global cardiovascular risk. PMID:28128312
Data suggest that serum CRP levels show positive association with ChildTurcottePugh scores in subjects with liver cirrhosis; GSH levels show negative association with ChildTurcottePugh scores; thus, CRP and GSH appear to exert synergistic effects in association with severity of liver cirrhosis. This cross-sectional study was conducted in Taiwan. (GSH = glutathione) PMID:29861471
Study shows for the first time that CRP activates signaling of integrin alpha2 via binding to integrin alpha2 and Fcgamma receptor I in breast cells and MDA-MB-231 triple-negative human breast cancer cells. PMID:28846105
This meta-analysis suggests that C-reactive protein/albumin ratio (CAR)may be a potential prognostic marker in solid cancers PMID:28128229
The results of the study suggest that the levels of C-reactive protein and tumor necrosis factor-alpha are important diagnostic markers of inflammation in patients with chronic pancreatitis and type 2 diabetes mellitus. PMID:30280549
Elevated serum C-reactive protein could be a bacterial infection predictor in decompensated cirrhosis. PMID:29384797
The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of Cardiac biomarkers . Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. PMID:29857573
Data suggest that C-reactive protein (CRP) may play an important role in the development of tongue squamous cell carcinoma (TSCC). PMID:29385406
For WBC, N (%), and ESR, both groups showed similar patterns of temporal values 14 days after surgery regardless of preoperative CRP level. Our findings could be used as guidelines for patient discharge and during the follow-up period after surgery. PMID:29854731
The neutrophil/lymphocyte ratio is a better addition to C-reactive protein than CD64 index as a marker for infection in COPD. PMID:28185443
This retrospective cohort study aimed to identify the early postoperative kinetics of C-reactive protein (CRP) and procalcitonin (PCT) in children undergoing tetralogy of Fallot (ToF) correction. PMID:29111402
After beta-blocker treatment, hs-CRP levels are associated with functional capacity in heart failure patients. PMID:29396658
Hypertensive patients with coronary tortuosity have a higher serum hs-CRP level concentration and have a higher incidence of lacunar infarction than hypertensive patients without coronary tortuosity. PMID:29436255
High CRP expression is associated with non-small cell lung cancer. PMID:29549910
T allele of rs1205 in CRP gene was protective against Takayasu arteritis in our patients. CC genotype was associated with involvement of coronary artery and ascending aorta. A trend toward a blunted CRP response seen in patients. PMID:29024426
Serum C-reactive protein (and COMP) levels were both associated with the incidence of knee osteoarthritis. PMID:29351749
coexistence of both higher HCY and higher hs-CRP in the acute phase of ischemic stroke were associated with subsequent PSD, independently of established conventional risk factors PMID:29325799
Data suggest that C-reactive-protein (CRP) concentration is weakly associated with global DNA methylation level, and this association was more clearly in individuals with the minor allele of the 5,10-methylenetetrahydrofolate reductase (MTHFR)missense SNP rs1801133. PMID:29439678
A longitudinal association between adolescent CRP levels and adult schizophrenia diagnosis indicates a potentially important role of inflammation in the pathogenesis of the illness PMID:27622678
rs1205 genetic variability in the CRP gene determines the CRP levels in sepsis of different severities PMID:29379005
CRP levels predict the development of anastomotic leak and postoperative mortality after colorectal cancer surgery. PMID:28132052
Uremia patients receiving maintenance hemodialysis with hospitalacquired infection had increased serum inflammatory factors and high throughput hemodialysis significantly decreased CRP, IL2 and TNFalpha levels in the serum, suggesting that high throughput hemodialysis may be beneficial for the prevention of the infections in uremia patients. PMID:29257244
High CRP expression is associated with cardiovascular disease. PMID:28724840
The results of this meta-analysis suggest that increased CRP expression indicates a poorer prognosis in patients with bone neoplasms. PMID:29668751
C-reactive protein (CRP) recognized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG) in the supported 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers without calcium at pH 7.4 and 5.5. PMID:29172154
Used Ultra Performance Liquid Chromatography-Ultra Definition Mass Spectrometry-based proteomics to compare serum samples from 19 colorectal cancer (CRC) patients. The samples were first analyzed after dividing them into two categories based on CRP, CRP <30 and CRP >30, and the same samples were analyzed again after being divided into the categories short and long 5-year survival. PMID:29630649
CRP bound to surface CD32 (also known as FcgammaRII) on myeloma cells, which activated a pathway mediated by the kinase p38 MAPK and the transcription factor Twist that enhanced the cells' secretion of osteolytic cytokines. PMID:29233917
high-sensitivity C-reactive protein could have a role in recurrent stroke and vascular events in patients treated with statins PMID:28302952
Procalcitonin and CRP serum levels are associated with the appearance of intra-abdominal infection after colorectal cancer surgery. PMID:28918433
C-reactive protein polymorphisms and DNA methylation of the AIM2 gene influence associations between trauma exposure, posttraumatic stress disorder, and C-reactive protein. PMID:28867284
Elevated CRP level is correlated with poor prognosis in patients with metastatic renal cell carcinoma receiving tyrosine kinase inhibitors treatment. (Meta-analysis) PMID:29080691
The study reveals that CRP might be a clear cell renal cell carcinoma development promoter. PMID:28923830
CRP distinguished sepsis and non-septic ICU patients. PMID:28898742
Suggest that hs-CRP may be a marker of comorbidities associated with worse health status in myocardial infarction. PMID:28946036