CFD Recombinant Monoclonal Antibody

Code: CSB-RA005271MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA

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Product Details
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Target Background

Product Details

Uniprot NO.

P00746

Target Names

CFD

Alternative Names

Complement factor D (EC 3.4.21.46) (Adipsin) (C3 convertase activator) (Properdin factor D) , CFD, DF PFD

Species Reactivity

Human

Immunogen

Recombinant Human CFD protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

3F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol

Recommended Dilution

Application	Recommended Dilution
ELISA	1:5000-1:50000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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The Binding Activity of Human CFD with Anti-CFD Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CFD (CSB-MP005271HU) at 2 μg/mL can bind Anti-CFD recombinant antibody. The EC₅₀ is 31.86-39.21 ng/mL.

Description

Complement factor D, also known as adipsin, serves as a rate-limiting serine protease in the alternative complement pathway, where it cleaves factor B bound to C3b to generate the active C3 convertase. Beyond its classical role in innate immunity, this protein has garnered significant attention for its involvement in adipocyte biology and metabolic regulation, making it a compelling target for researchers investigating complement-mediated diseases, obesity, and inflammatory conditions.

This recombinant monoclonal antibody against human CFD offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with clone 3F10, this antibody provides a sequence-defined reagent that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The human IgG1 isotype format and affinity chromatography purification ensure high purity suitable for sensitive detection methods.

Functional validation demonstrates robust binding activity in ELISA applications, with the antibody recognizing immobilized human CFD protein at concentrations as low as 2 μg/mL. The determined EC50 range of 31.86–39.21 ng/mL indicates strong binding affinity, allowing researchers to use this antibody across a broad dilution range of 1:5000 to 1:50000 depending on their specific assay requirements and detection systems.

Supplied in a stabilized liquid format containing 50% glycerol and PBS at pH 7.4 with Proclin 300 preservative, this antibody maintains activity through proper storage at -20°C or -80°C while minimizing freeze-thaw cycles. Researchers studying complement pathway regulation, adipokine signaling, or developing therapeutic strategies targeting alternative pathway activation will find this antibody a reliable tool for their quantitative immunoassay applications.

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Target Background

Function(From Uniprot)

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Gene References into Functions

MASP-3 is the exclusive pro-factor D activator in resting blood: the lectin and the alternative complement pathways are fundamentally linked. PMID:27535802
disturbance of reciprocal relationships between adipsin and leptin in obesity is associated with the development of insulin resistance PMID:28864895
TZDs (pioglitazone, rosiglitazone, and ciglitazone) slow tumor cell growth in vitro and in vivo with decreases in cell proliferation and increases in PPARg and adipsin PMID:28219679
The strong association of PLIN1, CFD and ADIPOQ genes with adipogenesis prompted authors to study the influence the bone health status as evaluated by quantitative ultrasound (QUS) bone densitometer in a North Indian cohort. Overall, ADIPOQ (rs1501299 and rs3774261) and combined cluster of PLIN1 rs2304796 and rs2304795) and CFD (rs1683563) demonstrated correlation. PMID:28189761
our findings set up a novel mechanism for FABP4, adipsin and adiponectin through gut microbiota mediating expression in gut Paneth cells. PMID:26687459
Overall adipsin levels among male asbestos industry workers were significantly higher than among control subjects. PMID:25840635
Data indicate that a urinary protein, adipsin, was significantly increased in patients with preeclampsia. PMID:24958499
Study demonstrates that T2DM patients with beta cell failure are deficient in adipsin. PMID:24995977
Complement factor D (FD) is activated by its substrate through interactions outside the active site which lock the unbound native state into an ordered inactive conformation via the self-inhibitory loop in FD. PMID:24598742
We demonstrate novel secretion of adipsin and ASP by placental Hofbauer cells. PMID:23956345
Increased activation of the alternative complement pathway in vitreous was controlled by disease stage and genetic variation in the complement pathway, supporting a role for complement activation in macular degeneration disease pathogenesis. PMID:22930722
Anti factor D Fab fragment inhibits FD proteolytic function by interfering with macromolecular substrate access rather than by inhibiting FD catalysis. PMID:22362762
CFD regulates activation of the alternative complement pathway, which is implicated in age related macular degeneration pathogenesis. PMID:22003108
CFD may not play a major role in the genetic susceptibility to age-related macular degeneration. PMID:21139680
crystal structure of C3bB at 4 A and complex with factor D at 3.5 A; data show how factor B binding to C3b forms open "activation" state of C3bB; Factor D binds open conformation of factor B through a site distant from the catalytic center PMID:21205667
As adiponectin and adipsin levels in CSF did not correlate with their levels in plasma, it seems that there could be a secondary intrathecal synthesis of these adipocytokines in multiple sclerosis PMID:19538214
increased serum levels in patients with seasonal allergic rhinitis PMID:19699327