Abbreviation
Recombinant Human CFD protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CFD at 2 μg/mL can bind Anti-CFD recombinant antibody (CSB-RA005271MA1HU). The EC50 is 31.86-39.21 ng/mL.
Alternative Names
Complement factor D; EC:3.4.21.46; Adipsin; C3 convertase activator; Properdin factor D; CFD;DF, PFD
Species
Homo sapiens (Human)
Expression Region
26-253aa
Target Protein Sequence
ILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CFD at 2 μg/ml can bind Anti-CFD recombinant antibody (CSB-RA005271MA1HU). The EC50 is 31.86-39.21 ng/mL.
Description
Complement factor D catalyzes the rate-limiting step of the alternative pathway by cleaving factor B within the C3bB complex, making it a critical control point for complement-mediated immune responses. This full-length mature protein (aa 26–253) demonstrates specific antibody binding with an EC50 of 31.86–39.21 ng/mL in functional ELISA, providing quantitative evidence that the recombinant protein retains native epitope presentation suitable for complement activation assays and inhibitor screening targeting the alternative pathway. The combination of >95% purity and <1.0 EU/μg endotoxin meets the quality thresholds commonly required for hemolytic assays and opsonization studies, where contaminating proteases or inflammatory triggers would confound interpretation of complement-dependent lysis or phagocytosis. Researchers investigating immune evasion mechanisms or validating complement ELISA protocols can use this C-terminal His-tagged protein as a positive control with defined binding characteristics.
