CD7 Recombinant Monoclonal Antibody

Code: CSB-RA436550A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P09564

Target Names

CD7

Alternative Names

T-cell antigen CD7 (GP40) (T-cell leukemia antigen) (T-cell surface antigen Leu-9) (TP41) (CD antigen CD7), CD7

Species Reactivity

Human

Immunogen

A synthesized peptide from human CD7 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9B3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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Western Blot
Positive WB detected in: Jurkat whole cell lysate(30µg)
All lanes: CD7 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 25 kDa kDa
Observed band size: 37 kDa kDa
Exposure time：2min

Description

CD7 is a transmembrane glycoprotein that plays a fundamental role in T-cell and natural killer cell biology, functioning as a costimulatory molecule involved in T-cell activation and intercellular signaling. As one of the earliest markers expressed during T-cell development, CD7 serves as a valuable tool for immunological research, particularly in studies of T-cell ontogeny, leukemia classification, and immune cell subset identification. Its consistent expression on T-cell acute lymphoblastic leukemia cells has also made it an important marker in hematological malignancy research.

This recombinant monoclonal antibody, generated from clone 9B3, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The affinity-chromatography purification ensures high purity for sensitive downstream applications.

Validation by western blot demonstrates specific detection of CD7 in Jurkat whole cell lysate, a well-established human T-cell leukemia line. The observed band at approximately 37 kDa runs higher than the predicted 25 kDa molecular weight, which is consistent with the extensive glycosylation characteristic of this membrane glycoprotein. Working dilutions between 1:500 and 1:2000 provide flexibility for optimization across different experimental conditions, with additional utility confirmed for ELISA applications.

This antibody is well-suited for researchers investigating T-cell biology, lymphocyte development, and hematological malignancies where reliable CD7 detection in human samples is essential for their experimental workflows.

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Target Background

Function(From Uniprot)

Not yet known.

Gene References into Functions

in acute myeloid leukemia patients the association of CD7 positivity and FLT3 positivity was found to be significant PMID:25679063
Data show that CD7 promotes extramedullary involvement of the B-ALL line Tanoue in an integrin beta2-dependent manner. PMID:24920488
We showed that the CADM1 versus CD7 plot is capable of discriminating clonally expanding HTLV-I-infected cells in indolent and acute-type T-cell leukemia-lymphomas and in asymptomatic carriers PMID:24727323
CD7 is present on monocytes and tumor macrophages and its ligand, SECTM1, is frequently expressed in corresponding melanoma tissues PMID:24157461
SECTM1 secreted from bone marrow stromal cells may interact with CD7 to influence GM-CSF expression in leukemic cells. PMID:24211252
epigenetic down-regulation of CD7 is associated with acute myeloid leukemia. PMID:20398252
CD7 loss in aggressive natural killer-cell leukemia; a useful diagnostic marker PMID:20046078
low expression in T-cell lymphomas due to Twist2-mediated suppression of promoter activity; enhanced by histone deacetylase inhibitors PMID:19937140
These findings indicate a link between epigenetic modifications and CD7 expression in primitive chronic myeloid leukemia cells. PMID:20175919
CD7 expression in hematopoitic cells denotes commitment to B-cell and natural killer cells lineages. PMID:12393702
Association of T cell antigen CD7 with type II phosphatidylinositol-4 kinase, a key component in pathways of inositol phosphate turnover. PMID:12594831
a novel fusion protein, designated scFvCD7:sFasL is designed to have leukemia-restricted activity. PMID:16332967
Patients expressing CD7 had significant shorter disease free (DFS) and post-remission survivals (PRS) than patients without CD7 (DFS of 12 months versus 42 months, P=0.005; PRS of 15 months versus 33 months, P=0.013). PMID:16837044
CD7 antigen notably expresses in lung microvascular endothelial cells (LME) and that it acts as an Fc receptor for IgM in LME cells. PMID:16990185
HTLV-IInfected cells acquired a profound decrease of intracellular calcium levels in response to ionomycin, timely correlated with decreased CD7 expression PMID:17287851
differential levels of CD7 identify the progressive stages of lineage commitment in human thymus, initiated from a primitive CD7(-) lympho-myeloid thymic progenitor PMID:17959857
close association of aberrant CD7 expression and FLT3/ITD mutation in the myeloblasts of FLT3/ITD+ acute myeloid leukemia suggests that FLT3/ITD- mediated leukemic transformation occurs in the more early stage of myeloid progenitor cells PMID:18343790
The CD7 expression in CD4(+) T cells discriminates between HL and reactive LAD, suggesting that this could be a useful and practical adjunctive tool in the diagnosis of Hodgkin lymphoma. PMID:18956470
by down-regulating CD7, ATLL cells could have escaped Gal-3-induced apoptosis to run a more aggressive clinical course PMID:19207946
the galectin-1 glycoprotein receptor CD7 maybe a novel target for GdA on T cells. PMID:19683346