CD48 Recombinant Monoclonal Antibody

Code: CSB-RA004941MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

P09326

Target Names

CD48

Alternative Names

Antigen CD48 antibody; B cell membrane protein antibody; B lymphocyte activation marker BLAST 1 antibody; B-cell activation marker antibody; B-lymphocyte activation marker BLAST-1 antibody; BCM 1 surface antigen antibody; BCM1 antibody; BCM1 surface antigen antibody; BLAST 1 antibody; BLAST antibody; BLAST1 antibody; CD 48 antibody; CD48 antibody; CD48 antigen (B cell membrane protein) antibody; CD48 antigen antibody; CD48 molecule antibody; CD48 protein antibody; CD48_HUMAN antibody; hCD48 antibody; Leucocyte antigen MEM 102 antibody; Leukocyte antigen MEM-102 antibody; mCD48 antibody; MEM 102 antibody; MEM-102 antibody; MEM102 antibody; Signaling lymphocytic activation molecule 2 antibody; SLAM family member 2 antibody; SLAMF 2 antibody; SLAMF2 antibody; TCT.1 antibody

Species Reactivity

Human

Immunogen

Recombinant Human CD48 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

6F9H2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing Jurkat cells surface stained with CSB-RA004941MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

CD48, also known as BLAST-1 or SLAMF2, serves as a critical cell surface glycoprotein within the SLAM family of immunomodulatory receptors. Expressed predominantly on hematopoietic cells, CD48 functions as a ligand for CD2 and 2B4, mediating essential cell-cell adhesion events that regulate lymphocyte activation, cytotoxicity, and immune surveillance. Its involvement in natural killer cell function and T cell co-stimulation makes CD48 a compelling target for researchers investigating immune regulation, hematological malignancies, and immunotherapy development.

This recombinant monoclonal antibody, clone 6F9H2, offers the reproducibility and consistency that demanding immunological research requires. Developed using recombinant technology with a defined human IgG1 framework, this antibody eliminates the lot-to-lot variability inherent in traditional hybridoma-derived reagents. Researchers can confidently design longitudinal studies and multi-site collaborations knowing that antibody performance will remain consistent across experiments.

Validated for flow cytometry applications, this antibody demonstrates clear detection of CD48 on human Jurkat cells, a well-established T cell leukemia line commonly used in lymphocyte biology studies. Flow cytometry validation at dilutions ranging from 1:50 to 1:200 showed distinct positive staining compared to isotype control, confirming specific recognition of surface CD48. The antibody has also been validated for ELISA applications, providing flexibility for researchers employing multiple detection platforms within their experimental workflows.

Supplied in a stabilized liquid format containing glycerol for convenient long-term storage, this antibody is well-suited for investigations into lymphocyte activation pathways, immune checkpoint biology, and the characterization of hematological cell populations where CD48 expression serves as a functionally relevant marker.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Gene References into Functions

soluble CD48 levels were significantly elevated in patients with nonallergic asthma compared to control and to the allergic asthma cohort PMID:30306094
mCD48 and sCD48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD48 inhibits CD244-mediated eosinophil activation. These findings suggest that CD48 may play an important role in human asthma. PMID:27859399
The present study provides further insights into the role of the 2B4-CD48 interaction in the fine regulation of CD8(+) T-cell effector function upon antigenic stimulation. PMID:26860368
Data show that 2B4 not only can bind to CD48 in trans but also interacts with CD48 in cis by using the same binding interface. Also, the results demonstrated that constitutive phosphorylation of 2B4 occurs only in the presence of CD48, and that cis binding is sufficient to induce substantial levels of baseline phosphorylation. PMID:27249817
Our data indicate sCD48 as a SEB-induced 'decoy' receptor derived from eosinophil and therefore as a potential anti-inflammatory tool in S. aureus-induced eosinophil inflammation often associated with allergy. PMID:26836239
CD48 expression was increased in patients with a short disease duration compared to both controls and patients with longer disease duration. In patients with short disease duration, increased CD48 expression was associated with alveolar inflammation. PMID:26926492
These data demonstrate the important role of CD48 in SA/exotoxins-eosinophil activating interactions that can take place during allergic responses and indicate CD48 as a novel therapeutic target for allergy and especially of AD. PMID:25255823
Blockade of 2B4/CD48 interaction resulted in improvement in function via perforin expression and degranulation as measured by CD107a surface mobilization on HTLV-1 specific CD8+ T cells. PMID:24505299
we propose that SLAMF2 engagement regulates adaptive immune responses PMID:24670806
Monocyte-induced natural killer cell dysfunction was markedly attenuated by blocking CD48 receptor 2B4 on NK cells, but not by blockade of NKG2D and NKp30. PMID:23225218
replication study of association of 2 SNPs in HERV-K18 and 19 tagSNPs in CD48 with schizophrenia (SZ)and type 2 diabetes (T2D) in patients with SZ in 2 Danish samples; no association was found with SZ or with T2D among individuals with SZ for any of the SNPs PMID:22495247
Stimulation of CD48 induces rearrangement of signaling factors in lipid rafts, Lck-kinase activity, and tyrosine phosphorylation. PMID:20833258
The ligand (CD48) of the 2B4 receptor can exert both activating and inhibiting signals; natural killer (NK) cells might be at risk for self-killing were it not for the inhibiting signals generated by the 2B4-CD48 interaction. PMID:20164429
CM1-induced apoptosis is achieved via different initiation pathways, which are cell-type dependent PMID:12072193
Signal-dependent adhesion of resting NK cells initiated by expression of ICAM-1 is greatly enhanced by coexpression of CD48, even in the absence of cytokines. PMID:12496412
Engagement of natural killer (NK) cell receptor 2B4 by its counterreceptor, CD48, expressed on target cells leads to an inhibition in NK cytotoxicity independent of signaling lymphocytic activation molecule-associated protein (SAP) expression. PMID:15356144
IL-18, IL-18 receptor alpha, and CD48 complex formation via glycosylphosphatidylinositol anchor glycan triggers binding to IL-18 receptor beta, and thereby induces intracellular signal transduction and IFN-gamma production. PMID:15760905
Review of recent studies suggests an important role for interactions between 2B4 and CD48 in the course of T cell activation and proliferation PMID:16081768
2B4 (CD244) can stimulate NK cell cytotoxicity and cytokine production by interacting with NK cell expressed CD48 and adds CD48 to the growing number of activating NK cell receptors PMID:16585556
CD48 is an interleukin (IL)-3-induced activating receptor on eosinophils and may be involved in promoting allergic inflammation. PMID:16785501
In conclusion, we cannot confirm a role of human endogenous retrovirus-K18 superantigen polymorphisms or of the CD48 CA repeat for type 1 diabetes susceptibility. PMID:16866884
findings indicate that FimH induces host cell signalling cascades that are involved in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC) and CD48 is a putative HBMEC receptor for FimH PMID:17222190
the mechanism of signal transduction by CD244 is to regulate FYN kinase recruitment and/or activity and the outcome of CD48/CD244 interactions is determined by which other receptors are engaged. PMID:17599905