CD2 Recombinant Monoclonal Antibody

Code: CSB-RA911142A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P06729

Target Names

CD2

Alternative Names

CD 2 antibody; CD2 antibody; CD2 antigen (p50), sheep red blood cell receptor antibody; CD2 antigen antibody; CD2 molecule antibody; CD2_HUMAN antibody; Erythrocyte receptor antibody; FLJ46032 antibody; LFA-2 antibody; LFA-3 receptor antibody; LFA2 antibody; LFA3 receptor antibody; Ly-37 antibody; Lymphocyte function antigen 2 antibody; lymphocyte-function antigen-2 antibody; OTTHUMP00000024366 antibody; Rosette receptor antibody; Sheep erythrocyte receptor antibody; SRBC antibody; T cell surface antigen CD2 antibody; T-cell surface antigen CD2 antibody; T-cell surface antigen T11/Leu-5 antibody; T-lymphocyte surface CD2 antigen antibody; T11 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CD2

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HepG2 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg)
All lanes: CD2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 24 kDa
Exposure time: 3min

Description

CD2, also known as LFA-2 or T11, is a cell surface glycoprotein that plays a fundamental role in T cell activation and adhesion. Expressed primarily on T lymphocytes and natural killer cells, CD2 mediates cell-cell interactions through binding with LFA-3 (CD58), facilitating the initial contact between T cells and antigen-presenting cells. This interaction is critical for immune synapse formation and subsequent T cell signaling, making CD2 a valuable marker for immunological research and studies investigating T cell-mediated immune responses.

This recombinant monoclonal antibody, clone 6F10, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity against human CD2, ensuring that results remain comparable across experiments and over time. The affinity-chromatography purification process delivers a reagent with minimal background interference.

Validated for Western blot applications, this antibody has demonstrated reliable detection across multiple human cell lines including HepG2, COLO205, and MCF-7 at dilutions ranging from 1:500 to 1:2000. In Western blot analysis, the antibody detects a band at approximately 24 kDa, which differs from the predicted molecular weight of 39 kDa. This size difference likely reflects proteolytic processing or detection of a specific CD2 isoform, as the protein undergoes post-translational modifications in cellular contexts.

This CD2 antibody serves researchers investigating T cell biology, immune cell adhesion mechanisms, and lymphocyte activation pathways. Its utility extends to studies examining tumor immunology and immune checkpoint regulation, where understanding T cell surface marker expression provides critical insights into immune function and dysfunction.

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Target Background

Function(From Uniprot)

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Gene References into Functions

A new prognostic model for HR-/HER2+ breast cancer based on the expression of MMP11 and CD2 was developed and the distant metastasis-free survival for patients in the high-risk group according to our model was significantly lower than that for those in the low-risk group. PMID:28409241
These results reveal an unexpected redundancy in the human NK cell response to human cytomegalovirus and suggest that CD2 provides "signal 2" in antibody-driven adaptive NK cell responses. PMID:27117418
Establish CD2 as a new susceptibility factor for systemic sclerosis in a European Caucasian population. PMID:27385538
the fundamental mechanism of glycosylation of human CD2 is to promote CD2-CD58 binding by conformational adjustment of CD2 PMID:25984915
CD58/CD2 is the primary costimulatory pathway in human CD28-CD8+ T cells. PMID:26041540
Both the mRNA expression levels and protein expression levels of HSP27 were increased in astrocytes from POAG patients compared with those from normal control, suggesting that mutation in CD2 might pose a risk for POAG in Chinese population. PMID:24597656
Although sMCs displayed immunoreactivity for one of the neoplastic antigens in the majority of SM patients, the aberrant CD2 and/or CD25 expression on sMCs is not as indicative of SM as the BMMC immunophenotype. PMID:25402852
ERGdel may be a pure surrogate of CD2-positivity--which has been suggested to be a good prognositc marker in childhood ALL PMID:24072102
Altogether, these results show that a high CD2 expression level is a hallmark of latently infected resting memory CD4(+) T cells in vivo. PMID:23760244
Aberrant CD2 expression appears to further determine a shorter progression free survival PMID:22634534
CD2-CD58/48 receptor-ligand interaction promotes and is required for nanotube formation in human natural killer cells. PMID:23112830
CD2 expression does not contribute to improve the diagnosis of systemic mastocytosis when compared with aberrant CD25 expression alone. PMID:22222639
CD2-mediated priming of resting natural killer (NK) cells is unaffected by their degree of functional maturation. PMID:22084431
CD2 signals more strongly to S6-ribosomal protein, whereas CD28 costimulation specifically induces signaling necessary for proper NF-kappaB activation. PMID:22013130
When isolated from multiple sclerosis patients, both nonmature and effector subsets of memory CD127(low) regulatory T cells exhibit kinetically distinct defects in suppression that are evident with CD2 pathway costimulation. PMID:21300823
analysis of the importance of homotypic NK-to-NK cell cross-talk through 2B4/CD48 and CD2/CD58 pairs and further present their differential and overlapping roles in human NK cells PMID:20813844
PTEN expression was up-regulated on RNA and protein level in freshly isolated human CD4(+) T cells following stimulation with CD28 or CD2. PMID:11932928
molecules redistribute to the uropod during T cell scanning PMID:12032326
Structural and functional studies of the extracellular domains of CD2 and CD58 and their complex. Review. PMID:12369898
CD2BP2 is the ligand of the membrane-proximal proline-rich tandem repeat of CD2 in detergent-soluble membrane compartments, but is replaced by Fyn SH3 after CD2 is translocated into lipid rafts upon CD2 ectodomain clustering. PMID:12426371
PSTPIP1 acts downstream of CD2/CD2AP to link CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. PMID:12530983
This T cell surface antigen is linked to the actin-capping protein CAPZ via CMS and CIN85. PMID:12690097
another role of the CD2-CD58 pathway that allows nonimmune and immune cells to interact directly with dendritic cells and initiate innate and adaptive immune responses. PMID:12714509
CD2 mediates activation of the IFN-gamma intronic STAT binding region in mucosal T cells. PMID:12731040
CD2 mediates activation of a distal -3.6-kilobase STAT5 binding region of the interferon-gamma promoter. PMID:15528362
CD48 is a CD2 and CD244 (2B4)-binding protein PMID:16803907
We use this analysis to determine that the 2D Kd for CD2-CD58 is 5.4-7.6 molecules/microm2. 2D Kd analysis provides a general and quantitative measure of the mechanisms regulating cell-cell adhesion. PMID:17085486
T cell activation causes the CD58-bound CD2 to be recognized and immobilized at sites of cell-cell contact, thereby strengthening T cell-APC adhesion PMID:17168569
results showed that the expression of CD2 significantly increased with the severity of chronic HBV infection, which suggested that CD2 might contribute to the hepatocyte damage in chronic HBV infection PMID:18318997
Data suggest that detection of CD2 or CD13 expression in chronic lymphocytic leukemia (CLL) suggests familial CLL, and that CD38 expression does not carry the negative prognosis observed in sporadic CLL. PMID:18431797
the synergistic synthesis of IL-8 occurs when lymphocytes are stimulated through the CD2 pathway by CD58 on HT-29 cells, resulting in TNF-alpha release that, in turn, augments IL-8 synthesis and CD58 expression by the HT-29 cells PMID:19109405
during clinical remission, increases in CD58 expression, mediated by the protective allele, up-regulate the expression of FoxP3 through engagement of the CD58 receptor, CD2, leading to the enhanced function of TREG cells that are defective in MS PMID:19237575
CD2 functions as the master switch recruiting CD48 and Lck PMID:19494291
LFA-1 and CD2 synergize for the Erk1/2 activation in the Natural Killer (NK) cell immunological synapse PMID:19502238
CD244 inhibition and activation depends on CD2 and phospholipase C-gamma1 PMID:19586919
Genetic variants at CD2 are associated with rheumatoid arthritis risk PMID:19898481
CD2-CD58 binding site PMID:11575926