Abbreviation
Recombinant Human CD2 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD2 at 5 μg/mL can bind Human CD58(CSB-MP6138HUj8-B). The EC50 is 337.0-430.1 ng/mL.
Alternative Names
T-cell surface antigen CD2; Erythrocyte receptor; LFA-2; LFA-3 receptor; Rosette receptor; T-cell surface antigen T11/Leu-5; CD2; SRBC
Species
Homo sapiens (Human)
Expression Region
25-209aa
Target Protein Sequence
KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD2 at 5 μg/ml can bind Human CD58(CSB-MP6138HUj8-B). The EC50 is 337.0-430.1 ng/mL.
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The purity of CD2 was greater than 95% as determined by SEC-HPLC
Description
CD2 mediates T-cell adhesion to antigen-presenting cells and target cells through its interaction with CD58 (LFA-3), a critical checkpoint in immune synapse formation and T-cell activation. This recombinant fragment spanning residues 25–209 captures the complete extracellular immunoglobulin-like domains responsible for ligand recognition and demonstrates quantifiable binding to human CD58 with an EC50 of 337.0–430.1 ng/mL in functional ELISA, confirming that the mammalian expression system has preserved the native conformation required for receptor-ligand engagement. The validated CD2-CD58 interaction supports use in cell adhesion assays examining T-cell conjugate formation, immune cell trafficking studies modeling lymphocyte homing, and antibody development programs targeting costimulatory pathways in cancer immunotherapy. Purity exceeding 95% by both SEC-HPLC and SDS-PAGE, combined with endotoxin levels below 1.0 EU/μg, meets the quality thresholds commonly required for sensitive binding assays and functional cell-based experiments.
