CCR7 Recombinant Monoclonal Antibody

Code: CSB-RA215587A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, IF

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Target Background

Product Details

Uniprot NO.

P32248

Target Names

CCR7

Alternative Names

C-C chemokine receptor type 7 (C-C CKR-7) (CC-CKR-7) (CCR-7) (BLR2) (CDw197) (Epstein-Barr virus-induced G-protein coupled receptor 1) (EBI1) (EBV-induced G-protein coupled receptor 1) (MIP-3 beta receptor) (CD antigen CD197), CCR7, CMKBR7 EBI1 EVI1

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human CCR7 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

22E12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Jurkat whole cell lysate(30µg), Raji whole cell lysate(30µg), A549 whole cell lysate(30µg), A431 whole cell lysate(30µg), K562 whole cell lysate(30µg), U-937 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), Mouse lung tissue lysate(30µg)
All lanes: CCR7 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time：20s
Immunofluorescence staining of A549 cell with CSB-RA215587A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Description

CCR7, also known as CD197, is a G-protein coupled chemokine receptor that plays a central role in directing immune cell trafficking and lymphoid tissue organization. This receptor guides the migration of dendritic cells and T lymphocytes to secondary lymphoid organs through its interaction with chemokine ligands CCL19 and CCL21, making it essential for adaptive immune responses. In cancer research, CCR7 expression has emerged as a significant marker associated with lymph node metastasis across multiple tumor types, positioning it as both a prognostic indicator and potential therapeutic target.

This recombinant monoclonal antibody, clone 22E12, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human CCR7, provides reliable detection across human and mouse samples.

Validation studies demonstrate robust performance in western blot applications, where the antibody detects a band at the predicted 43 kDa molecular weight. Testing across diverse sample types confirms versatility, with positive detection in Jurkat, Raji, A549, A431, K562, U-937, and HL-60 cell lysates, as well as mouse lung tissue, supporting cross-species reactivity for comparative studies. Immunofluorescence validation in A549 cells reveals clear cellular localization patterns, enabling visualization of CCR7 distribution in fixed cell preparations.

Whether investigating immune cell homing mechanisms, characterizing tumor metastatic potential, or exploring inflammatory responses, this antibody provides a dependable tool for researchers working at the intersection of immunology and cancer biology.

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Target Background

Function(From Uniprot)

Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.

Gene References into Functions

Results suggest that chemokine (C-C motif) receptor 7 (CCR7)promotes triple-negative breast cancer (TNBC) metastasis and may serve as a target for breast cancer diagnosis and treatment. PMID:30032244
High CCR7 expression is associated with urinary bladder cancer metastasis. PMID:28534984
these data indicate CCL19/CCR7 contributes to proliferation and invasion of ESCs, which are conducive to the pathogenesis of endometriosis through activating PI3K/Akt pathway PMID:28856757
The research findings demonstrate for the first time that the chemokines CCL19, CCL21 and CCR7 play important roles in bone destruction by increasing osteoclast migration and resorption activity, and that has been linked to rheumatoid arthritis pathogenesis. PMID:28729639
CXCR4, CCR7, VEGF-C and VEGF-D expression might have synergistic effects on the lymph node metastasis in patients with cervical cancer. PMID:28535405
the acute GvHD (aGvHD) patients received higher percentage of CD4+CCR7+ T-cells in donor T-cells, whereas chronic GvHD (cGvHD) patients were transplanted with higher percentages of CD8+CCR7+ T-cells. Functional experiments demonstrated that CCR7+ T-cells exhibited higher potential for activation than CCR7- T-cells did. PMID:28112745
these results demonstrated that CCL21/CCR7 may activate EMT in lung cancer cells via the ERK1/2 signaling pathway. PMID:28487957
CCL21/CCR7 interaction was shown to allow NK cell adhesion to endothelial cells (ECs) and its reduction by hypoxia. PMID:28416768
Study shows that miR-1275 can positively regulate CCR7 expression in squamous cell carcinoma of head and neck (SCCHN) with different mechanisms. PMID:29278769
Study provide evidence that CCR7 mediates EMT progress via AKT pathway, which indicates that CCR7 has a key role in breast cancer progression. PMID:28378417
Report an increased percentage of peripheral CCR7 T cells accompanied by endothelial dysfunction in patients with ankylosing spondylitis. PMID:28421995
The analysis of gene amplification and mRNA levels showed the expression of CCR7 in breast cancer correlated with better prognosis. PMID:28930757
that CCR7 mediates TGF-beta1-induced MMP2/9 expression through NF-kappaB signaling PMID:28817313
leukocyte subsets express distinct patterns of CCR7 sialylation that contribute to receptor signaling and fine-tuning chemotactic responses. PMID:26819318
tumor microenvironment stimulation down-regulated the migration of CCR7-expressing tumor cells toward CCL21 and inhibited the formation of directional protrusions toward CCL21 in a novel 3-dimensional hydrogel system. PMID:26936935
CCR7 expression levels in human tumors correlate with signatures of CD141(+) DC, intratumoral T cells, and better clinical outcomes. PMID:27424807
specific role for CCL21/CCR7 in promoting EMT and metastasis in CD133+ pancreatic cancer stem-like cells PMID:27505247
This study showed that CCR7 are overexpressed in CD4(-) CD8(-) thymocytes of myasthenia gravis patients. PMID:26616645
High tumoral CCR7 expression correlated with potential lymphatic involvement and poor prognosis of metastatic renal cell carcinoma patients treated with tyrosine kinase inhibitors. PMID:28114889
Results show that upregulation of CCR7 promotes cell proliferation and inflammation in A549 non-small cell lung cancer cells. However, silencing of CCR7 via siRNA treatment promotes cell apoptosis and suppresses the inflammatory response and TGF-beta1-induced EMT, which may be associated with NF-kappaB signaling. PMID:28339080
Down-regulating CCR7 expression in MG63 cells could apparently inhibit cell proliferation, migration and invasion abilities of MG63 cells, and also induce cell apoptosis. PMID:27916085
These results suggest that upregulation of rat CCR7 expression does not change the phenotype, differentiation, or proliferation capacity of human adipose-derived stem cells (hASCs), but does enable efficient migration of hASCs to rat secondary lymphoid organs. PMID:28035134
CCL21/CCR7 interaction contributes to the time-dependent proliferation of PTC cells by upregulating cyclin A, cyclin B1 and cyclin-dependent kinase 1 (CDK1) expression via the extracellular signal-regulated kinase (ERK) pathway associated with iodine. PMID:27574129
we conclude that CCR7 gene locus harbours a polymorphism that modifies risk of MI in patients with Coronary artery disease (CAD). Replication of this association could be sought in a prospective cohort of initially healthy individuals PMID:27317472
this study shows that plasmacytoid dendritic cells from rheumatoid arthritis patients have high expression levels of CCR7 PMID:27421624
This study suggests that NRP1 expression and LVD are independent factors that are likely to predict the risk of LN metastasis in squamous cell carcinoma (SCC)of the tongue, whereas the expression of VEGFC, VEGFR3, CCR7, and SEMA3E are nonindependent predictive factors PMID:27666723
High CCR7 expression is associated with gastric cancer. PMID:26984468
CCR7 pathway up-regulates Twist expression via ERK and PI3K/AKT signaling to manage the epithelial-mesenchymal transition of pancreatic ductal adenocarcinoma PMID:26219899
Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages. PMID:26923638
High CXCR4 expression in primary breast tumors (PTs) was found to be associated with luminal A type tumor, suggesting more favorable outcome. In contrast, CCR7 and FOXP3 expressions in PTs represented luminal B tumors, pointing to more aggressive tumor behavior. Maspin expression did not differ between luminal types. PMID:28011488
Results indicated that CCR7 is overexpressed in gallbladder cancer tissues and its expression correlates with staging and lymph node metastasis. PMID:27009073
CCL21/CCR7 induce VEGF-D up-regulation and promote lymphangiogenesis via ERK/Akt pathway in lung cancer. PMID:26884842
The present study demonstrated that down-regulation of CCR7 reduced proliferation, cell cycle, cell migration and invasion in prostate cancer cells PMID:26722441
Our findings suggested that MUC1 plays an important role in CCL21-CCR7-induced lymphatic metastasis and may serve as a therapeutic target in esophageal squamous cell carcinoma . PMID:26667143
Membrane associated PGES1dependent release of PGE2 is ccompanied by elevated CCR7 expression in colon cancer cells. PMID:26352871
Pyk2 is a key downstream signaling molecules of CCR7 in SCCHN, which promotes SCCHN tumorigenesis and progression. PMID:26352169
the CCR7 axis mediates TGF-beta1-induced EMT via crosstalk with NF-kappaB signaling, facilitating lymph node metastasis and poorer overall survival in patients with gastric cancer PMID:26176983
Blockade of CCR7, or treatment with a p38 MAP kinase inhibitor, reduced lymphatic dissemination of epithelial-mesenchymal transition cells. PMID:25961925
findings indicated that circulating memory Tfh cells, especially CCR7+ICOS+ memory Tfh cells, may be associated with the relapse of MS and may serve as a new therapeutic target PMID:26231034
The chemotactic interaction between CCR7 and its ligand, CCL21, may be a critical event during progression in pancreatic cancer PMID:21594558
CrkL regulates CCL19 and CCR7-induced epithelial-to-mesenchymal transition via ERK signaling pathway in epithelial ovarian carcinoma patients. PMID:25636509
Recycling accounts, to a major extent, for the high levels of surface CXCR4/CCR7 on CLL cells. Increased CCR4/CCR7 is detectable not only in circulating leukemic cells, but also in secondary lymphoid organs of CLL patients with lymphoadenopathy. PMID:26282174
in given donor/recipient pairs, KIR2DS4 expression may contribute to potentiating natural killer cell function by increasing both the cytotoxicity and the expression of CCR7 on their surface. PMID:25961063
Data indicate that inhibition of TGF-beta-activated protein kinase 1 (TAK1) reduces chemokine (C-C motif) receptor 7 (CCR7) expression. PMID:25557171
CCL21/CCR7 pathway activates signallings to up-regulate Slug pathway, leading to the occurrence of epithelial-mesenchymal transition process in human chondrosarcoma PMID:25556164
our study suggested that CCR7 promotes Snail expression to induce the epithelial-mesenchymal transition, resulting in cell cycle progression, migration, and invasion in gastric cancer PMID:25572817
High CCR7 expression was associated with colorectal cancer metastasis. PMID:26179425
The solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. PMID:26115234
These results reveal that CCR7 and VEGF-C display a significant crosstalk and suggest a novel role of the CCL21/CCR7 chemokine axis in the promotion of breast cancer-induced lymphangiogenesis. PMID:25744065
High expression of CCR-7 is associated with atherosclerotic arteries. PMID:25318003

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

Database Links

HGNC: 1608

UNIGENE: Hs.370036

KEGG: hsa:1236

STRING: 9606.ENSP00000246657

OMIM: 600242

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