BGLAP Recombinant Monoclonal Antibody

Code: CSB-RA11839MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

P02818

Target Names

BGLAP

Alternative Names

Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein), BGLAP

Species Reactivity

Human

Immunogen

Recombinant Human BGLAP protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

16A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Overlay Peak curve showing U87 cells stained with CSB-RA11839MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

BGLAP, commonly known as osteocalcin, serves as a critical marker in bone biology research, functioning as the most abundant non-collagenous protein in bone matrix. Secreted exclusively by osteoblasts, this vitamin K-dependent protein plays essential roles in bone mineralization and calcium homeostasis. Beyond its skeletal functions, osteocalcin has emerged as a fascinating research target due to its endocrine activities, including regulation of glucose metabolism and energy expenditure, making it relevant to studies spanning osteoporosis, metabolic disorders, and bone-related diseases.

This recombinant monoclonal antibody, clone 16A8, offers researchers the reproducibility and consistency that recombinant technology provides. Unlike traditional hybridoma-derived antibodies, this sequence-defined reagent ensures identical performance across lots, eliminating batch-to-batch variability that can compromise longitudinal studies or multi-site collaborations. The human IgG1 isotype format and affinity-chromatography purification deliver a highly specific reagent suitable for demanding experimental workflows.

Validated for ELISA and flow cytometry applications, this antibody demonstrates reliable detection of human BGLAP. Flow cytometry validation using U87 cells confirms specific intracellular staining when cells are fixed and permeabilized, with recommended dilutions ranging from 1:50 to 1:200. The validation data shows clear separation between antibody-stained and isotype control populations, indicating robust signal-to-noise performance for quantitative cellular analysis.

Whether investigating osteoblast differentiation, exploring osteocalcin's metabolic signaling functions, or developing diagnostic assays for bone turnover markers, this antibody provides a dependable tool for advancing your research into bone biology and its broader physiological connections.

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Target Background

Function(From Uniprot)

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Gene References into Functions

The results of this study suggest that serum osteocalcin is associated with the onset of puberty in girls. PMID:30089739
The correlation between Mycobacterium avium subsp. paratuberculosis (MAP) infection and elevation of sera ucOC, reduction of active osteocalcin and increased calcium supports MAP infection role in Crohn's disease and complications with osteoporosis. PMID:29638140
The results showed no significant correlation between osteocalcin HindIII gene polymorphism and bone mineral density (BMD) and therefore osteocalcin HindIII gene polymorphism may not have major effects on BMD variation in postmenopausal North Indian women. PMID:30179426
Decreased circulating carboxylated osteocalcin is associated with low-energy bone fractures in children. PMID:29882816
a decrease in serum osteocalcin levels is associated with future development of type 2 diabetes mellitus. PMID:28766135
Findings from the available data indicated an overall significant inverse association between serum osteocalcin and body mass index--{REVIEW} PMID:28822067
relatively lower serum osteocalcin levels were associated with a higher risk of MACE in Chinese men with CAD PMID:28921598
undercarboxylated form of osteocalcin in serum not related significantly to semen parameters or measured hormones in male infertility PMID:28000970
serum level not correlated with gender, race/ethnicity, age, diabetes type, BMI percentile, random C-peptide, glucose, or HbA1c at pediatric diabetes diagnosis; serum level inversely correlated with HbA1c at first office visit following diagnosis PMID:28093839
Serum osteocalcin was directly and independently correlated with circulating uric acid in a population of healthy obese subjects. PMID:28847266
gamma-carboxylation of OCN on Glu17 residue correlates with insulin resistance and subclinical inflammation, suggesting that gamma-carboxylation of OCN negatively regulates its endocrine action PMID:28242268
There were differences in the associations between total osteocalcin and glucose metabolism in Han and Uygur T2DM patients, indicating genetic factors may play a role in modulating OC and glucose metabolism in different ethnic population. PMID:28732499
The study demonstrated that undercarboxylated osteocalcin (ucOC) is related to 25(OH)D and adiponectin concentrations. Both ucOC and 25(OH)D were lower in overweight and obese adolescents. PMID:28443718
Increased advanced glycation end products and protein oxidative damage markers in the course of obesity may contribute to decreased carboxylated osteocalcin serum level. PMID:28841723
Progressive calcification of atherosclerotic plaques is accompanied by insignificant accumulation of calcitonin and osteoprotegerin, whereas the osteocalcin expression significantly increased during calcification. PMID:28429221
Undercarboxylated osteocalcin reflects the reserve capacity of beta-cell function, such as the bolus insulin secretion ability in patients with type 2 diabetes. PMID:27889949
XPS showed that similar amounts of hOC and dhOC were absorbed onto hydroxyapaptite and octacalcium phosphate surfaces, but ToF-SIMS detected some small differences in the amino acid fragment intensities on these surfaces for adsorbed hOC and dhOC. PMID:28521505
Common polymorphism rs1800247 in osteocalcin gene was associated with hypertension and diastolic blood pressure levels. The association of rs1800247 with hypertension was affected by its interaction with HOMA-IR. PMID:27251081
These findings indicate the undercarboxylated form of OC is not implicated in age-related changes in skeletal muscle or adipose tissue mass in older community-dwelling adults. PMID:27604070
Demonstrate a positive association between osteocalcin, a factor known to regulate bone remodelling, with cognitive performance in older non-demented women. PMID:27515675
Common genetic variants of the osteocalcin gene may not be a major contributor to variations in serum osteocalcin or BMD in postmenopausal and elderly Chinese women. PMID:28002816
Several detected relations between OCN and metabolites were strongly linked to renal function, however, a number of associations remained significant after adjustment for renal function. Intermediates of proline catabolism were associated with OCN reflecting the implication in bone metabolism. PMID:28922389
Low serum osteocalcin concentrations are associated with type 2 diabetes. PMID:27061949
Decreased blood concentration of Carboxylated osteocalcin is associated with metabolic complications and inflammation. PMID:27667744
Total and undercarboxylated osteocalcin levels were significantly correlated with insulin secretion and insulin resistance indices, but not adiponectin levels, in pregnant women. PMID:26708046
Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A. PMID:27673554
Osteocalcin plays an essential role for the maturation of mineral species and modulates osteogenic differentiation of mesenchymal stromal cells (MSCs). PMID:28106724
Serum osteocalcin levels in pediatric obstructive sleep apnea hypopnea syndrome patients were significantly lower than normal controls before adenotonsillectomy. PMID:26938749
the relationship between serum osteocalcin and glucose metabolism parameters in liver transplantation recipients, was examined. PMID:26743527
Reduced plasma Osteocalcin levels are associated with cardiovascular and metabolic diseases. PMID:26863345
An overview of osteocalcin progress. PMID:26747614
the BMP-2/Smad1/5/RUNX2 signaling pathway participates in the silicon-mediated induction of COL-1 and osteocalcin synth PMID:27025722
Data indicate that lower osteocalcin was an independent predictor of worse cognitive performance on multivariate analysis. PMID:26406918
A common polymorphism rs1800247 in osteocalcin gene may effect the risk of osteoporosis and fracture and serum osteocalcin levels in Chinese. PMID:26194493
There is an inverse relationship between the visfatin and undercarboxylated osteocalcin in type 2 diabetes patients with diabetic kidney disease. PMID:26922969
This study investigated osteocalcin, metabolic parameters and anthropometric characteristics in normal weight and overweight/obese children. PMID:26772621
Nonalcoholic fatty liver disease negatively associated with right-hip bone mineral density and serum osteocalcin in Korean men. PMID:26636404
The aims of this study were to evaluate OCN and sclerostin levels in subjects who underwent coronary artery bypass graft (CABG) surgery compared with those in normal controls. PMID:26576474
serum level not associated with weight loss or body fat percentage PMID:25963022
Serum osteocalcin levels are inversely correlated with nonalcoholic fatty liver disease in postmenopausal Chinese women with normal blood glucose levels. PMID:26567728
Serum total osteocalcin level is significantly lower in 2 diabetes mellitus patients than controls. PMID:26372899
Elevated serum preptin and decreased osteocalcin concentrations, together with insulin resistance, are associated with obesity and overweight. PMID:25675353
gestational diabetes is not associated with osteocalcin, under-carboxylated osteocalin, osteopontin and leptin and does not correlate with insulin resistance. PMID:25480426
Serum uOC levels in non-dialysis patients with CKD are significantly lower than those in healthy individuals, and uOC is closely associated with subclinical atherosclerosis in CKD patients. PMID:26381729
Endocrine function of osteocalcin and the cell biology events that allow osteocalcin to become a hormone. [review] PMID:25577163
Data indicate that higher osteocalcin (OC) or its undercarboxylated form (ucOC) concentration and ucOC to osteocalcin ratio (OCR) were associated with the risk of coronary calcification in men. PMID:25868823
Data suggest that a low serum osteocalcin level was an independent risk factor for abdominal obesity. PMID:25871789
Correlation between osteocalcin-positive endothelial progenitor cells and spotty calcification in patients with coronary artery disease PMID:25644945
These findings unequivocally document that increased vitamin K intake reduces the uncarboxylated form of OCN PMID:25855755
Serum osteocalcin level was not independently associated with C-IMT in a metabolically healthy Chinese population. PMID:26077201