BAX Recombinant Monoclonal Antibody

Code: CSB-RA002573MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, WB, IHC, FC

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Target Background

Product Details

Uniprot NO.

Q07812

Target Names

BAX

Alternative Names

Apoptosis regulator BAX (Bcl-2-like protein 4) (Bcl2-L-4), BAX, BCL2L4

Species Reactivity

Human

Immunogen

Recombinant Human BAX protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

24D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), HEK293 whole cell lysate(20µg), A549 whole cell lysate(20µg), THP-1 whole cell lysate(20µg)
All lanes: BAX antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3min
IHC image of CSB-RA002573MA1HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing Hela cells stained with CSB-RA002573MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

BAX, also known as Bcl-2-like protein 4, serves as a critical pro-apoptotic regulator within the intrinsic mitochondrial death pathway. As a member of the Bcl-2 protein family, BAX plays an essential role in determining cell fate by promoting mitochondrial outer membrane permeabilization, leading to cytochrome c release and subsequent caspase activation. Understanding BAX expression and function is fundamental to research in cancer biology, where dysregulated apoptosis contributes to tumor development and therapeutic resistance, as well as in neurodegenerative disease and developmental biology studies.

This recombinant monoclonal antibody, clone 24D12, offers the reproducibility and consistency that demanding research applications require. Generated using recombinant technology with a sequence-defined approach, this antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents, ensuring your experimental results remain comparable across extended studies.

Validated across multiple detection platforms, this antibody provides flexibility for diverse experimental workflows. Western blot analysis demonstrates clean detection of BAX at the expected 21 kDa molecular weight across multiple human cell lines, including HeLa, HEK293, A549, and THP-1, confirming reliable performance in both adherent and suspension cell models. Immunohistochemistry validation in human testis tissue sections shows specific staining patterns suitable for tissue-based expression studies. Flow cytometry analysis in HeLa cells reveals distinct positive population shifts compared to isotype controls, enabling quantitative assessment of intracellular BAX levels at the single-cell level.

This antibody is particularly well-suited for researchers investigating apoptotic signaling mechanisms, screening therapeutic compounds targeting cell death pathways, or characterizing BAX expression patterns in human disease models.

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Target Background

Function(From Uniprot)

Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Gene References into Functions

Pro-apoptotic protein Bax is main effector of mitochondrial permeabilization during apoptosis. Data suggest Bax N-terminal acetylation by mouse NatB (or yeast Naa20p) is involved in mitochondrial targeting of Bax. These studies used recombinant human Bax expressed in yeast cells plus cultured embryonic cells from knockout, transgenic, and chimeric mice. (NatB and Naa20p = N-terminal acetyltransferases) PMID:29233735
This result suggests that p53 has an important role in hemangioma endothelial cell (HemEC) apoptosis. The results of the present study additionally suggest that the propranololinduced HemEC apoptosis pathway is a mitochondrial apoptosis pathway and is regulated by p53BAX signaling. PMID:29767244
The results of the present study revealed that the combination of P. gingivalis and H1N1 infection in lung epithelial cells may promote the production of inflammatory cytokines and increase NO production, leading to increased levels of apoptosis in lung epithelial cells via the Bcl2/Bax/caspase3 signaling pathway. PMID:29750299
Bax activator BTC-8 inhibited glioblastoma (GBM) cell proliferation, arrested the cell cycle, and induced apoptosis through the induction of mitochondrial membrane permeabilization. Most importantly, BTC-8 blocked proliferation and self-renewal of glioma stem cells (GSC) and induced their apoptosis. BTC-8 was demonstrated to sensitize both GBM cells and GSCs to the alkylating agent Temozolomide. PMID:28368610
BAX nuclear localization was associated in vivo with the remodelling of lung parenchyma during development, tumorigenesis as well as fibrosis compared to control adult human lungs PMID:28933587
There is no significant association between BAX gene polymorphism and cancer susceptibility, but it probably contributes to increased adverse prognosis to cancer. PMID:30024563
These results suggest that chlorogenic acid (CGA)suppresses hLECs apoptosis and prevents lens opacity induced by H2O2 via Bax/Bcl2 signaling pathway. CGA may provide effective defenses against oxidative stress and, thus, has potential as treatment for a variety of diseases in clinical practice. PMID:29207051
Ru(II)/diphenylphosphine/pyridine-6-thiolate complexes induce S-180 cell apoptosis through intrinsic mitochondrial pathway involving inhibition of Bcl-2 and p53/Bax activation. PMID:28795366
helix alpha9 assists Bax activation via the dimer heterogeneity and interactions with specific MOM lipids, which eventually facilitate proteolipidic pore formation in apoptosis regulation PMID:27381287
VDAC2 ensures mitochondria-specific membrane association of Bax and in the absence of VDAC2 Bax localizes towards other cell compartments. Bax retrotranslocation is also regulated by nucleotides and calcium ions, suggesting a potential role of the transport of these ions through VDAC2 in Bax retrotranslocation. PMID:27620692
The results of the genes expression analysis revealed that indocyanine green-photodynamic therapy at concentrations 1000mug/mL, induced the significant expression of BAX in HGF cells PMID:28438509
Our observations point to misfolded Bax states, shedding light on the molecular mechanism of Bax mutation-elicited cancer. Most importantly, the structure of the Bax pore facilitates future study of releases cytochrome C in atomic detail PMID:27630059
Data suggest that regulation of pancreatic beta-cell function and survival/apoptosis involves alternative splicing modulated by key splicing regulator SRP55; SRP55-regulated alternative splicing includes modulation of function of pro-apoptotic proteins (BIM, BAX), JNK signaling, and endoplasmic reticulum stress. (SRP55 = pre-mRNA-splicing factor SRP55; BIM = BCL-2 interacting protein BIM) PMID:29246973
High expression of BAX is associated with colorectal cancer. PMID:28586030
High mitochondrial Bax apoptosis regulator protein (BAX) levels correlate with improved acute myeloid leukemia (AML) patient survival. PMID:28420723
parkin-dependent targeting of misregulated BAX on the mitochondria provides substantial protection against BAX apoptotic activity. PMID:28760928
our data present preliminary evidence that inherited abnormalities in the intrinsic apoptosis pathway, related to BAX G(-248)A and BCL2 C(-717)A SNPs, are associated with treatment response and act as independent prognostic factors in DLBCL. PMID:27098707
SFRP5 confers protection against oxidative stress-induced apoptosis through inhibition of beta-catenin activation and downregulation of Bax. PMID:28834606
The ratio of Bax/Bcl-2 was significantly enhanced by the Ginsenoside Rg3 to Paclitaxel. PMID:28231544
this study show that PATZ1 expression correlates positively with BAX and negatively with BCL6 and survival in human diffuse large B cell lymphomas PMID:27494852
YY1 promotes apoptosis via upregulating Bax transcription and subsequent activation of Bax by translocation from the cytosol to the mitochondrial membrane. PMID:27074573
Immunohistochemical analysis showed that STAT3, GRP78 and BAX protein levels in the combination group were significantly higher than those in STAT3 group and CDDP group (P<0.05). Exogenous STAT3 and CDDP may synergistically inhibit the xenograft tumour growth through up-regulation of BAX protein via GRP78. PMID:27129294
This is the first study evaluating the potential relationship between BCL2 and BAX gene polymorphisms and RRD in a Greek population, showing a significant association between BAX rs4645878 polymorphism and RRD susceptibility. This finding suggests that an apoptotic mechanism is implicated in the pathogenesis of RRD PMID:28877516
Heavy ion irradiation could induce p53(-/-) hepatoma cells to undergo apoptosis via E2F1/Bax/Casp3 signaling pathway. PMID:28500630
Bax effects were dependent on its oligomeric state. Monomeric Bax did not affect the membrane, oligomeric Bax lowered the breakthrough force of the membrane, which in the context of pore formation, implies a lowering of the line tension at the edge of the pore. PMID:27755971
The C-terminal helical conformation of Bax, not its primary sequence, appears to be critical for CASP8 recruitment and activation culminating in cell death. PMID:28807790
An autoinhibited dimeric form of BAX regulates the cytosolic BAX activation pathway. PMID:27425408
Although TP53 and BAX immunoreactivity levels were associated with some clinicopathological parameters of the patients, the expression of EP300, TP53 and BAX did not reveal any prognostic significance in ccRCC PMID:28551630
Cells expressing mitoCERT import ceramides into mitochondria and undergo Bax-dependent apoptosis. PMID:27888218
Results support the hypothesis that the mitochondrion-specific lipid cardiolipin functions as a first contact site for Bax during its translocation to the mitochondrial outer membrane in the onset of apoptosis. In addition, dye leakage assays revealed that different oxidized phospholipids species in the mitochondrial outer membrane-mimicking vesicles can have opposing effects on Bax pore formation. PMID:28538152
The substitution of proline 168 for alanine favors Bax oligomerization. PMID:28322731
Data show that the increased acetylation of Ku autoantigen 70kDa (Ku70) in sirtuin 6 protein (SIRT6)-depleted cells disrupt its interaction with Bax apoptosis regulator protein (Bax), which finally resulted in Bax mitochondrial translocalization. PMID:28238784
Rhaponticum carthamoides extracts from transformed and normal roots increased the ratio of Bax/Bcl-2 proteins and increased TP53 levels to reduce glioma cell proliferation. PMID:27696406
these results indicate that EVA71 infection directly impacts the mitochondrial apoptotic pathway by modulating the recruitment and activation of Bax. PMID:28073399
the present study demonstrated that DAPK contributed to the Hcyinduced endothelial apoptosis via modulation of Bcl2/Bax expression levels and activation of caspase 3 PMID:27633052
The present study evaluates the prognostic role of the p53, Bax, Bcl-2 and cyclin E immunoexpression in colon cancer. PMID:27151692
The high expression of the BAX gene seems to be a negative regulator of autophagy in colorectal cancer cells. The relative downregulation of autophagy-related genes was observed in colorectal cancer samples. PMID:28035578
High expression of BAX is associated with hepatocellular carcinoma. PMID:27699664
Low BAX/BCL2 mRNA is associated with laryngeal squamous cell carcinoma. PMID:27129795
It was concluded that ginsenoside Rh2-O induced apoptosis of HepG2 cells through activation of the lysosomal-mitochondrial apoptotic pathway involving the translocation of Bax to the lysosome. PMID:27120618
Findings reveal a novel mechanism by which p53 utilizes TFIIS.h to selectively promote the transcriptional elongation of the bax gene, upsurging cell death in response to severe DNA damage. PMID:27005522
elevated MMP-2 expression and disturbance balance of Bcl-2/BAX expressions may be associated with the development and maintenance of atrial fibrillation. PMID:27141955
TG2 can inhibit tumor cell apoptosis through down-regulation of Bax and prevention of release Cyt C from mitochondria into cytoplasm PMID:25561282
Bcl-2 and Bax expression was significantly associated with histologic grade and clinical stage, which are classic factors of poor prognosis. We suggest the use of these proteins as potential prognostic markers in STS of extremities PMID:25906122
The aim of this study was to determine the expression of apoptotic factors Bax, Bcl-2, and Caspase-3 in lens epithelial cells (LECs) from cataracts secondary to pars plana vitrectomy with silicone oil (SO) tamponade. PMID:26956740
The expression of PHF20 was associated with Bax expression. PMID:26722404
Deletion of chromosomal region 19q13.1-13.4 is common in hereditary non-polyposis colorectal cancer (CRC). This deletion could be the cause of the reduction in the expression of the BAX gene observed in CRC. PMID:26228962
miR-449 regulates the SIRT1/p53/BAX pathway, which may be its possible mechanism in modulating cell apoptosis of cisplatin-induced Acute kidney injury. PMID:26968221
Polymorphism of BAX and TP53 genes may be potential genetic modifiers for developing ovarian cancer. PMID:26209050
BimEL-Bax pro-apoptotic cascade is activated by cAMP signalling of Bordetella adenylate cyclase toxin through SHP-1 phosphatase in phagocytes. PMID:26334669

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Subcellular Location

[Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm.; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.; [Isoform Delta]: Cytoplasm.

Protein Families

Bcl-2 family

Tissue Specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lu

Database Links

HGNC: 959

UNIGENE: Hs.624291

KEGG: hsa:581

STRING: 9606.ENSP00000293288

OMIM: 600040

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