BAG2 Recombinant Monoclonal Antibody

Code: CSB-RA104772A0HU

Rate （N/A）
Review （0）

Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

O95816

Target Names

BAG2

Alternative Names

BAG family molecular chaperone regulator 2 (BAG-2) (Bcl-2-associated athanogene 2), BAG2

Species Reactivity

Human

Immunogen

A synthesized peptide from human BAG2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11B5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), PC-3 whole cell lysate(30µg)
All lanes: BAG2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time：1min

Description

BAG2 serves as a critical co-chaperone that regulates protein quality control pathways, working alongside HSP70 family members to influence protein folding, degradation, and cellular stress responses. This molecular chaperone regulator has gained significant research attention for its roles in proteasomal degradation, tau protein homeostasis relevant to neurodegenerative disease, and emerging connections to cancer biology where it may influence tumor suppressor pathways.

This recombinant monoclonal antibody, clone 11B5, offers the reproducibility essential for longitudinal studies and multi-site collaborations. Because the antibody sequence is defined and production occurs in controlled recombinant systems, you can expect consistent performance across different lots—eliminating the variability that can complicate data interpretation when working with traditional hybridoma-derived antibodies.

Validation by western blot demonstrates reliable detection of BAG2 across multiple human cell lines, including HeLa, Jurkat, and PC-3 lysates. The antibody produces a clean signal at the expected 24 kDa molecular weight, confirming specific recognition of the target protein without apparent degradation products or non-specific bands under standard conditions. This performance across epithelial, T-lymphocyte, and prostate carcinoma cell backgrounds suggests robust utility for researchers working with diverse experimental models. The validated dilution range of 1:500 to 1:2000 for western blotting provides flexibility to optimize signal intensity based on your specific sample conditions and detection systems.

Whether investigating chaperone-mediated autophagy, exploring BAG2's influence on protein aggregation disorders, or examining its potential contributions to oncogenic signaling, this antibody provides a dependable tool for characterizing BAG2 expression and regulation in human experimental systems.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.

Gene References into Functions

the nucleotide exchange factor (NEF) Bag2 stimulates SV40 release from Hsc70, thereby enabling successful virus arrival at the cytosol, which leads to infection. Hsp105, another NEF of Hsc70, displays a function overlapping that of Bag2, underscoring the importance of this release reaction. PMID:29769335
A comprehensive review of the structure, functions, and protein interactions of BAG2 PMID:28536620
Data show that BAG2 Inhibits CHIP-Mediated HSP72 ubiquitination in aged cells. PMID:28042827
repression of BAG2 expression or BAG2 activity by cold-sensitive pathways, as modeled in undifferentiated and differentiated cells, respectively, may be a causal factor in the accumulation of cytotoxic hyperphosphorylated tau protein. PMID:26208804
NF-kappaB-mediated modulation of BAG2 expression regulates the shift between the neurotrophic and neurotoxic effects of Abeta1-42. PMID:25985852
Thus, BAG2 promotes mutant p53 accumulation and gain-of-function in tumor growth, metastasis and chemoresistance. PMID:26271008
This study showed that BAG2 (Bcl-2 associated athanogene family protein 2) and BAG5 (Bcl-2-associated athanogene family protein 5) stabilise pathogenic ataxin3-80Q by inhibiting its ubiquitination PMID:25006867
this data correlate BAG2 to PINK1 for the first time, strengthening the important role of BAG2 in Parkinson disease -related neurodegeneration. PMID:24383081
BAG2 binds to the carboxyl terminus of Hsp70-interacting protein (CHIP) and provides a cochaperone-dependent regulatory mechanism for preventing unregulated ubiquitylation of misfolded proteins by CHIP PMID:16169850
The activity of BAG-2 resembles that of the previously characterized Hsc70 cochaperone and CHIP inhibitor HspBP1. PMID:16207813