AQP1 Recombinant Monoclonal Antibody

Code: CSB-RA975044A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human, Mouse

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

P29972

Target Names

AQP1

Alternative Names

AQP 1 antibody; AQP CHIP antibody; AQP-1 antibody; AQP1 antibody; AQP1_HUMAN antibody; aquaporin 1 (channel-forming integral protein; 28kDa; CO blood group) antibody; aquaporin 1 (Colton blood group) antibody; Aquaporin CHIP antibody; Aquaporin-1 antibody; Aquaporin-CHIP antibody; Aquaporin1 antibody; Channel forming integral protein 28kDa antibody; Channel like integral membrane protein 28 kDa antibody; CHIP 28 antibody; CHIP28 antibody; CO antibody; Colton blood group antibody; Growth factor induced delayed early response protein antibody; MGC26324 antibody; Urine water channel antibody; Water channel protein CHIP 29 antibody; Water channel protein CHIP29 antibody; Water channel protein for red blood cells and kidney proximal tubule antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human AQP1 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19G6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate(30µg)
All lanes:Aquaporin 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 29 kDa
Observed band size: 40 kDa
Exposure time：2min
IHC image of CSB-RA975044A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

Aquaporin-1 (AQP1) serves as a critical water channel protein that facilitates rapid osmotic water transport across cell membranes, playing essential roles in kidney proximal tubule function, red blood cell physiology, and fluid homeostasis throughout the body. As a member of the aquaporin family, AQP1 is also recognized as the molecular basis for the Colton blood group antigen system, making it relevant to both basic cell biology and translational research in renal physiology, vascular biology, and beyond.

This recombinant monoclonal antibody, generated from clone 19G6 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures reliable results across experiments.

Validation testing demonstrates robust performance across multiple applications. In western blot analysis of NIH/3T3 whole cell lysate, the antibody detects a band at approximately 40 kDa when used at 1:1000 dilution. While the predicted molecular weight for AQP1 is 29 kDa, the observed higher molecular weight is consistent with post-translational glycosylation commonly reported for this membrane protein. For immunohistochemistry, the antibody has been validated in paraffin-embedded human pancreatic tissue at 1:100 dilution using citrate buffer antigen retrieval, producing clear specific staining. The antibody demonstrates cross-species reactivity with both human and mouse samples, offering flexibility for researchers working across model systems.

Whether investigating water transport mechanisms, studying signal transduction pathways, or exploring tissue-specific expression patterns, this antibody provides a reliable tool for AQP1 detection in your research.

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Target Background

Function(From Uniprot)

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Gene References into Functions

Case-control analyses reveal significant overrepresentation of rare variants in ATP13A3, AQP1 and SOX17, and provide independent validation of a critical role for GDF2 in heritable pulmonary arterial hypertension. PMID:29650961
Study demonstrated that a region upstream of the AQP1 gene (- 2300 to - 2200 bp) contains an enhancer required for pH-mediated regulation of AQP1 gene expression. PMID:29554889
H19 acted as AQP1 ceRNA in regulating miR-874. PMID:30021355
Data show that miR-320 negatively regulates aquaporin 1 (AQP1) expression by targeting its 3' untranslated regions (3'-UTR). PMID:29538612
The present study represents further confirmation of the hypothesized prognostic role of AQP1, which seems a reliable prognostic indicator. PMID:29495596
AQP1 may serve a role in glioblastoma multiforme migration, invasion and vasculogenic mimicry formation PMID:29257313
Adenovirus-mediated human AQP1 expression in hepatocytes improves lipopolysaccharide-induced cholestasis in rats. PMID:29087027
AQP1 may interact with VEGFA and play a role in vasculogenic mimicry, especially under hypoxic conditions, but the heterogeneity of malignant mesothelioma cells may result in different dominant pathways between patients. PMID:29104239
AQP1, 3 and 5 expression through the stages of human salivary gland morphogenesis, was examined. PMID:28766180
AQP1 was elevated in the majority of ovarian cancer tissues. Notably, the present study, to the best of our knowledge, suggests for the first time that AQP1 exhibits a key role in the viability, apoptosis, migration and invasion of ovarian cancer cells. PMID:28849036
AQP1 is expressed in atherosclerotic lesion neovasculature in human and mouse arteries and AQP1 deficiency augments lesion development in angiotensin II-promoted atherosclerosis in mice. PMID:28129470
Strong aquaporin 1 protein expression was an independent adverse prognosticator in hilar cholangiocarcinoma. PMID:27143047
Findings suggest that a defined population of AQP4- and AQP1-expressing reactive astrocytes may modify alpha-syn deposition in the neocortex of patients with Parkinson's Disease. PMID:26919570
The BRAF V600 mutations were significantly associated with AQP1 expression (P=0.014). Long-term follow-up indicated a reduced progression-free survival (P=0.036) and overall survival (P=0.017) for the AQP1-positive cutaneous melanoma patients. PMID:26848795
The expression of AQP1 and 3 is a manifestation of physiological adaptation of functionally mature chondrocytes able to respond to the change of their internal environment influenced by extracellular matrix. PMID:28708257
function of AQP1 in tonicity response could be coupled or correlated to its function in band 3-mediated CO2/HCO3(-) exchange PMID:28596233
Data show that the expression of aquaporin (AQP) 1, AQP3, AQP5, epithelial Na+ channel (ENaC) and sodium potassium ATPase (Na-K-ATPase) are altered in patients with acute respiratory failure (ARF) due to diffuse alveolar damage (DAD), and the cause of DAD does not seem to influence the level of impairment of these channels. PMID:27835672
The native AQP1 gene, normally latent in human salivary gland cells, can be activated by both promoter-independent artificial transcription and epigenetic editing of the promoter. PMID:28072927
data suggest an important functional role of AQP1 in the pathobiology of hypoxia-induced pulmonary hypertension PMID:28409279
We demonstrated that AQP1-like immunoreactivity and expression are significantly increased in plaques of Peyronie's disease patients Vs controls, implying that AQP1 overexpression might be the consequence of a localized maladaptive response of the connective tissue to repeated mechanical trauma PMID:26972451
AQP-1 overexpression is associated with prostate cancer progression. PMID:27817002
Study provides evidence that Mef2c cooperated with Sp1 to activate human AQP1 transcription by binding to its proximal promoter in human umbilical cord vein endothelial cells indicating that AQP1 is a direct target of Mef2c in regulating angiogenesis and vasculogenesis of endothelial cells. PMID:26923194
AQP1 was overexpressed in cystic cholangiocytes of patients with polycystic liver disease. A tendency of increased AQP1 protein expression in correlation with the cyst size was also found. PMID:26838488
Aquaporin1 overexpression restored anti-cell proliferation and metastatic effect of si-Gli1. PMID:27157540
AQP1 expression was significantly lower in hereditary spherocytosis patients and silent carriers than in the simultaneously processed normal control, while no AQP1 decrease was detected in the autoimmune hemolytic anemia sample. The decreased AQP1 expression could contribute to explain variable degrees of anemia in hereditary spherocytosis. PMID:27465156
High cytoplasmic expression of AQP1 is associated with breast cancer progression. PMID:26812884
Suggest that sputum AQP1 and AQP5 could be used as diagnostic markers in mild-to-moderate adult-onset asthma. PMID:26823734
the downregulation of AQP1 impedes extracellular to intracellular fluid transportation. PMID:26549133
Based on a comparative mutagenic analysis of AQP1, AQP 3, and AQP 4, the results suggest that loop D interactions may provide a general structural framework for tetrameric assembly within the AQP family. PMID:26786101
The role of AQP1 in malignant pleural mesothelioma. PMID:26773069
Urinary AQP1 is significantly lower in patients with clear cell renal carcinoma than in controls. Urinary AQP1 increases after nephrectomy in clear cell renal carcinoma patients. PMID:27295912
AQP1 knockdown can effectively inhibit cell proliferation, adhesion, invasion and tumorigenesis by targeting TGF-beta signaling pathway and focal adhesion genes. PMID:26176849
These data provide support for a continuous role of KLF2 in stabilizing the vessel wall via co-temporal expression of eNOS and AQP1 both preceding and during the pathogenesis of atherosclerosis. PMID:26717516
The study demonstrated the abnormal expression pattern of AQP1, AQP2, AQP3, and AQP4 in the kidney tissues of patients with nephrotic syndrome, providing a basis for an improved understanding of the role of aquaporins in the pathogenesis of this disease. PMID:26261083
The results suggest that AQP1 may facilitate lung cancer cell proliferation and migration in an MMP-2 and-9-dependent manner PMID:26151179
PBEF regulates the expression of inflammatory factors and AQP1 through the MAPK pathways. PMID:26178576
the benign subependymomas aquaporins 1 and 4 are dramatically redistributed and upregulated. PMID:26115524
AQP1 was found to be a marker of a subgroup of aggressive basaloid-like squamous cell carcinomas PMID:26074259
High AQP1 expression is associated with recurrence in bladder uroepithelial cell carcinoma . PMID:25987071
The AQP1 rs1049305 single nucleotide polymorphism is associated with running performance, but not relative body weight change, during South African Ironman Triathlons. PMID:25495276
The data suggest that the increased expression of AQP1 and AQP3 in pterygial tissues may be involved in the pathogenesis of pterygia PMID:23974882
CAII increases water conductance through AQP1 by a physical interaction between the two proteins. PMID:25609088
Aquaporin 1 and 3 were upregulated in cervical cancer compared to mild cervicitis and cervical intraepithelial neoplasia 2-3 (P<0.05). PMID:24918928
Through molecular genotyping we also identified polymorphisms in RhCE, Kell, Duffy, Colton, Lutheran and Scianna loci in donors and patients. PMID:25582271
Study found a significant correlation between AQP1, AQP3, and AQP5 overexpression and lymph node metastasis in patients with surgically resected colon cancer. PMID:25721378
the reduction of aquaporins 1 and 3 may be a factor resulting in lumbar intervertebral disc degeneration. PMID:25299207
AQP1 over-expression effectively inhibited cell proliferation and induced cell growth arrest in G1 phase of K562 cells. PMID:25252847
Aquaporin-1 is induced in leukocytes of patients with sepsis and exhibits higher expression in septic shock. PMID:24028651
Report is the first to establish astrocytic water channel loss in a subset of human central pontine myelinolysis (CPM) cases and suggests AQP1 and AQP4 may be involved in the pathogenesis of CPM PMID:24252214
Aquaporin-1 is associated with arterial capillary proliferation and hepatic sinusoidal transformation contributing to portal hypertension in primary biliary cirrhosis. PMID:23949237

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

MIP/aquaporin (TC 1.A.8) family

Tissue Specificity

Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.

Database Links

HGNC: 633

UNIGENE: Hs.76152

KEGG: hsa:358

STRING: 9606.ENSP00000311165

OMIM: 107776

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