AOC3 Recombinant Monoclonal Antibody

Code: CSB-RA624122MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

Q16853

Target Names

AOC3

Alternative Names

Amine oxidase; copper containing 3 (vascular adhesion protein 1) antibody; AOC3 antibody; AOC3_HUMAN antibody; Copper amine oxidase antibody; HPAO antibody; Membrane copper amine oxidase antibody; Membrane primary amine oxidase antibody; Semicarbazide sensitive amine oxidase antibody; Semicarbazide-sensitive amine oxidase antibody; SSAO antibody; VAP-1 antibody; Vascular adhesion protein 1 antibody; VP97 antibody

Species Reactivity

Human

Immunogen

Recombinant Human AOC3 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG4

Clone No.

3D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA624122MA1HU diluted at 1：150 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA624122MA1HU diluted at 1：150 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

AOC3, also known as vascular adhesion protein 1 (VAP-1), is a membrane-bound copper-containing amine oxidase that plays a dual role in vascular biology. Beyond its enzymatic function in oxidizing primary amines, AOC3 serves as an adhesion molecule that mediates leukocyte trafficking across endothelial barriers. This unique combination of enzymatic and adhesive properties has made AOC3 a compelling target in inflammation research, metabolic disease studies, and investigations into vascular pathology. Its expression patterns in endothelial cells and adipose tissue position it at the intersection of neuroscience and signal transduction research.

This recombinant monoclonal antibody, clone 3D3, offers the reproducibility that demanding experimental workflows require. Generated using recombinant technology with a defined sequence, this antibody delivers the lot-to-lot consistency essential for longitudinal studies and multi-site collaborations. The human IgG4 isotype format, combined with affinity chromatography purification, ensures high specificity for your human AOC3 detection needs.

Validation in immunohistochemistry demonstrates reliable performance across diverse human tissue types. Testing in paraffin-embedded human lung and adrenal gland tissues at 1:150 dilution using citrate buffer antigen retrieval confirms robust staining suitable for anatomical expression studies. The recommended working range of 1:50 to 1:200 provides flexibility to optimize signal intensity based on your specific tissue and detection system requirements. The antibody is also validated for ELISA applications, expanding its utility across different experimental platforms.

Whether you are investigating AOC3's role in leukocyte extravasation, exploring its enzymatic contributions to oxidative stress, or mapping its tissue distribution patterns, this recombinant antibody provides a dependable tool for advancing your research.

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Target Background

Function(From Uniprot)

Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.

Gene References into Functions

The study showed that serum VAP-1 level increased with an increasing severity of obstruction in patients with stable COPD. This increase was associated with a reduced quality of life and increased severity of hypoxia. PMID:29229088
VAP-1 expression is increased in primary sclerosing cholangitis, facilitates adhesion of gut-tropic lymphocytes to liver endothelium in a substrate-dependent manner, and elevated levels of its circulating form predict clinical outcome in patients. PMID:28428344
immunohistochemistry revealed that VAP-1 localized to endothelial cells of intra-plaque neovessels in carotid endarterectomy samples from patients with recent ischemic symptoms PMID:27731409
Adipose tissue SSAO activity did not vary according to anatomical location and/or metabolic status in severely obese women. PMID:27766585
Correlation among soluble receptors for advanced glycation end-products, soluble vascular adhesion protein-1/semicarbazide-sensitive amine oxidase (sVAP-1) and cardiometabolic risk markers in apparently healthy adolescents: a cross-sectional study PMID:27300745
This study showed that the oxidase activity of AOC3 contributes to the development of lung fibrosis mainly by regulating the accumulation of pathogenic leukocyte subtypes, which drive the fibrotic response. PMID:28251930
The mechanism of the Siglec-9 and AOC3 interaction PMID:27893774
psoriatic patients with the dominant AT1R-A1166C polymorphism had significantly higher serum levels of VAP-1 and MDA but lower total bilirubine, uric acid, ARE, SOD and CAT activities compared to the corresponding control group PMID:26395033
These findings suggest that vaspin and VAP-1 may play a role in the pathogenesis of psoriasis and can be used as markers of the disease PMID:25279492
Our results show that colorectal myofibroblasts, as defined by the expression of AOC3, NKX2-3, and other markers, are a distinctly different cell type from TGFbeta-activated fibroblasts PMID:27036009
In this article, we will review the general characteristics and biological functions of VAP-1, focusing on its important role as a prognostic biomarker in human pathologies. [review] PMID:26287391
serum VAP-1 can predict ESRD and is a useful biomarker to improve risk stratification in type 2 diabetic subjects. PMID:26845338
It stimulates amyloid beta proteins on the vascular walls, and is contributing to cerebral amyloid angiopathies and Alzheimer disease. PMID:25457560
Higher serum VAP-1 and Chronic kidney disease can independently predict future development of cancers in type II diabetic subjects PMID:24781952
Complexes of human ORP2 and VAPs at endoplasmic reticulum-lipid droplet interfaces regulate the hydrolysis of triglycerides and lipid droplet turnover. PMID:25420878
these results link the amine oxidase activity of VAP-1 with hepatic inflammation and fibrosis and suggest that targeting VAP-1 has therapeutic potential for NAFLD and other chronic fibrotic liver diseases. PMID:25562318
the semicarbazide-sensitive amine oxidase activity of VAP-1 modifies hepatic glucose homeostasis and may contribute to patterns of GLUT expression in chronic disease. PMID:25342050
This study demonstrated that low serum VAP-1 levels are associated with poor prognosis in gastric cancer patients. PMID:24903765
Serum VAP-1 predicts mortality independently and improves risk stratification in colorectal cancer subjects. PMID:24211727
SSAO expression in endothelial cells increases oxygen-glucose-deprivation-associated cell damage. It mediates some tissue damage during the reoxygenation process by oxidizing its known enzymatic substrate, methylamine. PMID:24503888
We concluded that VAP-1, elevated in patients on Peritoneal Dialysis, was predominantly dependent on residual kidney function and glucose level, factors both linked to endothelial damage and cardiovascular complications. PMID:24379274
VAP-1 elevations in heart transplant recipients were predominantly dependent on left ventricular diameter and use of tacrolimus PMID:23769096
Data suggest that subjects with diabetic retinopathy have higher serum levels of soluble VAP1 than subjects with non-angiogenic ocular diseases (i.e., epiretinal membrane [ERM], idiopathic macular hole [MH], age-related macular degeneration [AMD]). PMID:23062326
Data suggest that the down-regulation of both the expression and enzymatic activity of VAP-1 may result from a dominant-negative effect caused by heterodimerization between VAP-1 and alternatively spliced VAP-1Delta3. PMID:23349812
In psoriatic patients, VAP-1 elevation correlated with elevation of Lp (a). This may be responsible for a higher prevalence of cardiovascular accidents in psoriatic patients. PMID:22753196
The pathogenetic link between the increase in SSAO activity and severity of neurological deficitin ischemic stroke patients was shown. PMID:23120772
high vascular SSAO/VAP-1 levels in human hippocampus may contribute to vascular degeneration PMID:22714978
Protein level of sVAP-1 was increased and correlated with HEL in the vitreous fluid of patients with PDR. PMID:22618595
The immunolocalization of VAP-1 varied in the histopathology of the conjunctiva, involving the pathology of inflammatory conjunctival disorders. PMID:22354146
We found increased VAP-1 activity and anchor protein syndecan-1 content in critically ill patients with septic shock PMID:22150439
Plasma VAP-1/SSAO activity is increased in intracranial hemorrhage and predicts neurological outcome, suggesting a possible contribution of the soluble protein in secondary brain damage. PMID:22133888
This review examines the biological functions of VAP-1, especially the role that this molecule might play in the establishment and progression of chronic liver disease. PMID:21512782
using an endothelial cell model, a description for the first time the involvement of the leucocyte-adhesion activity of SSAO/VAP-1 in the Abeta (amyloid beta-peptide)-mediated pro-inflammatory effect PMID:21819380
The Siglec-9 peptide binding to the enzymatic groove of VAP-1 can be used for imaging conditions, such as inflammation and cancer. PMID:21821708
These remarks suggest that MAO-A and SSAO may play an important role in vascular tissue as well as in the vascular pathophysiology of type 2 diabetes. PMID:21114364
Met211 and Leu469 were shown to be key residues for substrate specificity. PMID:21585208
Serum VAP-1 can predict 10-year all-cause mortality, cardiovascular mortality, and cancer mortality independently in type 2 diabetic subjects. PMID:21282368
The structure of VAP-1 was refined to a resolution of 2.9A. PMID:21139198
A novel combination of surface molecules, including VAP-1 and CX(3)CL1 promotes the recruitment of CD16(+) monocytes to the liver, allowing them to localize at sites of chronic inflammation and fibrosis. PMID:20512991
The SSAO activity in the serum of patients with type 2 diabetes is higher when compared with control group and may be a useful marker for diabetes. PMID:20063021
immunohistochemistry reveals constitutive expression of VAP-1 in human ocular tissues. PMID:19761765
Inflammation of the dental pulp was accompanied by a significant decrease in MAO labeling, MAO B (but not MAO A) activity and the increase in SSAO activity. PMID:19789505
VAP-1 is expressed on hepatic sinusoidal endothelial cells in vitro and supports adhesion and transmigration of lymphocytes across these cells under physiological shear stress. PMID:12097405
Data show that increases in vascular adhesion protein-1 (VAP-1) due to absolute or relative insulin deficiency may be directly involved in the pathogenesis of diabetic angiopathy. PMID:12466139
normal mice and human lungs revealed vascular adhesion protein-1 expression in the endothelium of large and mid-sized pulmonary vessels PMID:12700900
may convert endogenous amines, like methylamine, to vasoactive metabolites. PMID:14715500
the oxidase activity of VAP-1 controls PMN exit from the blood during the relatively poorly understood transmigration step PMID:14726375
Semicarbazide-sensitive amine oxidase is a source of oxidative stress in diseased human skeletal muscle; it contributes to oxidative stress-induced damage in various inflammatory and other myopathies. PMID:14755492
VAP-1 protein expression is significantly decreased in intratumoral vessels compared with peritumoral ones; this difference was independent of the skin melanoma thickness PMID:15057044
Plasma semicarbazide-sensitive amine oxidase (SSAO) is elevated in patients with type 1 and type 2 diabetes and has been implicated in the pathophysiology of diabetic late complications. PMID:15830186

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Protein Families

Copper/topaquinone oxidase family

Tissue Specificity

Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, p

Database Links

HGNC: 550

UNIGENE: Hs.198241

KEGG: hsa:8639

STRING: 9606.ENSP00000312326

OMIM: 603735

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