Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
B-lymphocyte surface antigen B1Bp35Leukocyte surface antigen Leu-16Membrane-spanning 4-domains subfamily A member 1; CD20
Species
Homo sapiens (Human)
Expression Region
141-297aa
Target Protein Sequence
IKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Yeast-expressed Recombinant partial-length Human B-lymphocyte antigen CD20 (MS4A1) contains the region of 141-297aa of human MS4A1. It was fused with a 6xHis-tag at the N-terminus. The purity of this protein is over 90% as measured by SDS-PAGE. On the reducing SDS-PAGE gel, this MS4A1 protein migrated to the molecular weight band of about 22 kDa. This observed molecular mass is slightly higher than the calculated one (20.0 kDa) due to glycosylation. Except for antibody synthesis, this recombinant MS4A1 protein also may be used in the studies of MS4A1-associated immunology.
CD20, encoded by the gene MS4A1, is a B cell differentiation antigen expressed from the pre-B to mature B cell stage, but lost upon terminal
differentiation into plasma cells. CD20 is physiologically directly required for efficient BCR signaling in B cells. It normally exists as homodimeric and homotetrameric oligomers on the cell surface and combines with other cell-surface and cytoplasmic proteins, contributing to the signal transduction. Currently, CD20 antigen has been considered as an ideal target for immunotherapy of B cell malignancies and B cell-mediated autoimmune diseases.
