Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
(B-lymphocyte surface antigen B1) (Bp35) (Leukocyte surface antigen Leu-16) (Membrane-spanning 4-domains subfamily A member 1) (CD20)
Species
Homo sapiens (Human)
Expression Region
1-297aa
Target Protein Sequence
MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
50 mM HEPES，0.15 M NaCl， 0.05% DDM，0.01% CHS，pH7.5
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/ml on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007A1HU), the EC50 is 4.034-6.968 ng/mL.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/ml on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC50 is 8.137-9.704 ng/mL.
Description
CD20 is a B-cell-specific surface marker that plays a central role in B-cell activation and is the primary target for therapeutic antibodies in B-cell malignancies and autoimmune disorders. This full-length construct (aa 1–297) expressed in mammalian cells demonstrates quantified binding to anti-CD20 recombinant antibodies with EC50 values of 4.034–6.968 ng/mL and 8.137–9.704 ng/mL in functional ELISA, confirming that the protein adopts a conformation recognized by clinically relevant antibody epitopes. These binding characteristics support its use in antibody development and validation workflows, including epitope mapping, affinity maturation screening, and biosimilar characterization studies targeting CD20-directed therapeutics. Produced with a C-terminal 10×His tag to facilitate immobilization and purification, the protein meets the >90% purity threshold typical for functional binding assays and provides a suitable basis for investigating CD20-mediated B-cell signaling mechanisms in cancer immunology research.
