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Recombinant Xenopus laevis DNA2-like helicase (dna2), partial

Recombinant Xenopus laevis DNA2-like helicase (dna2), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

dna2

Purity

>85% (SDS-PAGE)

Target Names

dna2

Uniprot NO.

Q8QHA5

Alternative Names

dna2; dna2lDNA replication ATP-dependent helicase/nuclease DNA2; DNA replication ATP-dependent helicase-like homolog) [Includes: DNA replication nuclease DNA2; EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2; EC 3.6.4.12)]

Species

Xenopus laevis (African clawed frog)

Source

Yeast

Expression Region

1-1053

Target Protein Sequence

MEPVSAECHL PPEDDLLEMM MEQSFTEPEE KSQDKPTRKI IPKTKLCKGV NNRYCVLHIK EVYAQREEKH LTITASQEGD DLELCILKDD WVALQIKPGD IIHLEGNCSV DNTWTISRDT GYLILYPDLL ISGTSIANGI RCLRRSVLSE KFKVCDKGSR QMLNGTMLHD IFQRATTCGF TDSVLQELAH HTVHGPKYLK EMYQLKLNQA DVMGEIQEYL PSLSKWATDF MTHPLNQQQI NRTKSTAGDP TETTKVSEFL DIEENIWSPR FGLKGKIDVT ARVKIHQKSK AHLKIMPLEL KTGKESNSIE HRSQVVLYTL LSQERREDPE AGLLLYLKTG NMYTVPGNRL DRRELLKIRN ELSYYLTNVL HKSDNGSKET TLASLPAMIA DRQACKYCSQ MRNCALYNRS VEQQTENCYI PPEMIPVVQK ETEHLTEDHL QYFRLWYLMC TLEANSKDSK MGRKNIWMMS SSEREEDGQC IGNLIRTGHV QTISDVQYLH SFQRRSGSVP ATNLASGDRV VVSGEERFLA LSTGYIKEVK DENITCILDR SLVKLPEDLL FRLDHEEGGG GLEFHLGNLS RLMENSSVSE KLRKLIIDFS KPNFVQHLSS ILPPDAKDIV ASILRGLNKP QKQAMKRVLL SKDYTLIVGM PGTGKTTTIC TLVRILYACG FSVLLTSYTH SAVDNILLKL KKFQVGFLRL GRTQKLHPDV QEFSEEEICK AKSIKSLSAL EELYNSQPVV ATTCMGVNHP IFTRRRFDFC IVDEASQISQ PICLGPLFFA DRFVLVGDHQ QLPPLVKSAE ARELGMSESL FKRLERNQEA VVQLTVQYRM NSKIMALSNK LVYEGRLECA SDRVSNAVVQ LPHIKTLLLE LEFRESQESM WIKDVLEPSN PVCFLNTEKI PALETEEKGG ISNWIEAKLV FHLTKLYLKA GCRPSDIGII APYRQQLKMI SNYFNSLSAS AVEVNTVDKY QGRDKSVIIV SFVRSNIDGK LGDLLKDWRR LNVALTRAKH KLIMLGCVPT LNRFDCLEQL ICNLKTENQI YDLPEGAHEH FPV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function.

Gene References into Functions

Data show that cleavage of free 5' DNA ends at clean double-strand breaks (DSBs) is mediated by DNA replication helicase/nuclease 2 (Dna2). PMID:28981724
Dna2 co-localizes in foci with RPA and is found in a complex with replication fork components And-1 and Mcm10. Dna2 interacts with the DSB repair and checkpoint proteins Nbs1 and ATM. PMID:20237432
Results provide strong evidence that xDNA2 is a major nuclease for the resection of DNA ends for homology-dependent DNA double-strand break repair in eukaryotes. PMID:18820296

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Subcellular Location

Nucleus. Mitochondrion.

Protein Families

DNA2/NAM7 helicase family

Database Links

UNIGENE: Xl.17390

KEGG: xla:378492

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