dna2 Antibody

Code: CSB-PA854469XA01XBE

Rate （N/A）
Review （0）

Size:

0.1ml

0.1ml1ml

US$Enquire

Quantity:

Species Reactivity: Xenopus laevis (African clawed frog)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

Q8QHA5

Target Names

dna2

Alternative Names

dna2 antibody; dna2lDNA replication ATP-dependent helicase/nuclease DNA2 antibody; DNA replication ATP-dependent helicase-like homolog) [Includes: DNA replication nuclease DNA2 antibody; EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2 antibody; EC 3.6.4.12)] antibody

Raised in

Rabbit

Species Reactivity

Xenopus laevis (African clawed frog)

Immunogen

Recombinant Xenopus laevis (African clawed frog) dna2 protein

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Datasheet & COA

Antibody FAQs

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function.

Gene References into Functions

Data show that cleavage of free 5' DNA ends at clean double-strand breaks (DSBs) is mediated by DNA replication helicase/nuclease 2 (Dna2). PMID:28981724
Dna2 co-localizes in foci with RPA and is found in a complex with replication fork components And-1 and Mcm10. Dna2 interacts with the DSB repair and checkpoint proteins Nbs1 and ATM. PMID:20237432
Results provide strong evidence that xDNA2 is a major nuclease for the resection of DNA ends for homology-dependent DNA double-strand break repair in eukaryotes. PMID:18820296

Hide All

Subcellular Location

Nucleus. Mitochondrion.

Protein Families

DNA2/NAM7 helicase family

Database Links

UNIGENE: Xl.17390

KEGG: xla:378492

Promotions