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Recombinant Schizosaccharomyces pombe Protection of telomeres protein 1 (pot1), partial

Recombinant Schizosaccharomyces pombe Protection of telomeres protein 1 (pot1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

pot1

Uniprot NO.

O13988

Alternative Names

pot1; SPAC26H5.06; Protection of telomeres protein 1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Source

Yeast

Expression Region

1-555

Target Protein Sequence

MGEDVIDSLQ LNELLNAGEY KIGELTFQSI RSSQELQKKN TIVNLFGIVK DFTPSRQSLH GTKDWVTTVY LWDPTCDTSS IGLQIHLFSK QGNDLPVIKQ VGQPLLLHQI TLRSYRDRTQ GLSKDQFRYA LWPDFSSNSK DTLCPQPMPR LMKTGDKEEQ FALLLNKIWD EQTNKHKNGE LLSTSSARQN QTGLSYPSVS FSLLSQITPH QRCSFYAQVI KTWYSDKNFT LYVTDYTENE LFFPMSPYTS SSRWRGPFGR FSIRCILWDE HDFYCRNYIK EGDYVVMKNV RTKIDHLGYL ECILHGDSAK RYNMSIEKVD SEEPELNEIK SRKRLYVQNC QNGIEAVIEK LSQSQQSENP FIAHELKQTS VNEITAHVIN EPASLKLTTI STILHAPLQN LLKPRKHRLR VQVVDFWPKS LTQFAVLSQP PSSYVWMFAL LVRDVSNVTL PVIFFDSDAA ELINSSKIQP CNLADHPQMT LQLKERLFLI WGNLEERIQH HISKGESPTL AAEDVETPWF DIYVKEYIPV IGNTKDHQSL TFLQKRWRGF GTKIV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.

Gene References into Functions

Thus, our results suggest that pik1(+) is required for the maintenance of circular chromosomes. PMID:29410177
The Pot1 DNA-binding domain is capable of binding DNA in at least two structurally distinct binding modes. PMID:25074378
Pot1 promiscuity is implemented through binding modes that thermodynamically compensate for base-substitutions through alternate stacking interactions and hydrogen-bonding networks. PMID:23201273
Results indicate an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide containing two telomeric repeats. PMID:21911358
The high affinity conserved DNA-binding activity of Pot1 protein is conferred by a DNA-binding domain consisting of two subdomains. PMID:21815629
The Pot1 (protection of telomeres) protein prevents access of telomerase to the 3'-ends of telomeres and protects telomeric DNA against exonucleolytic degradation. [pot1] PMID:15637058
Pot1(1-389) presents a new model for studying and understanding the ssDNA-binding activity of S. pombe Pot1 and illustrates the malleability of both the ssDNA ligand and the oligosaccharidce-binding fold in the recognition and binding of telomeric ssDNA. PMID:16842820
Considering that the antiparallel tetraplex inhibits telomerase-mediated telomere elongation, we conclude that the ability of Pot1 to unfold the antiparallel tetraplex is required for telomerase-mediated telomere regulation. PMID:17158862
Characterization of the thermodynamics and accommodation of both cognate and noncognate telomeric single-stranded DNA (ssDNA) sequences by Pot1pN, an autonomous ssDNA-binding domain (residues 1-187) found in full-length S. pombe Pot1. PMID:18355038
study shows Tpz1 forms a complex with Pot1; Tpz1 binds to Ccq1 & Poz1 which protect telomeres redundantly & regulate telomerase in positive & negative manners; thus, Pot1-Tpz1 complex accomplishes its functions by recruiting effector molecules Ccq1 & Poz1 PMID:18535244
model in which conformational selection and induced fit play important roles in the recognition of ssDNA by Pot1pN (residues 1-187 of full-length Pot1) PMID:19232358