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Recombinant Schizosaccharomyces pombe Mitosis inhibitor protein kinase wee1 (wee1), partial

Recombinant Schizosaccharomyces pombe Mitosis inhibitor protein kinase wee1 (wee1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

wee1

Uniprot NO.

P07527

Alternative Names

wee1; SPCC18B5.03Mitosis inhibitor protein kinase wee1; EC 2.7.10.2; P107 protein kinase homolog

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Source

Yeast

Expression Region

1-877

Target Protein Sequence

MSSSSNTSSH RSYGLRRSQR SMNLNRATLL APPTPSSLYD ANNSTSSTSS QKPNTSFTSL FGPRKQTTSS PSFSHAAPLH PLSPPSFTHS QPQIQAQPVP RRPSLFDRPN LVSRSSSRLG DSPSLSPVAQ VANPIHHTAP SPSDVRAFPI HKNASTGVKR SFFSSSMSNG AMSPPSHSPS PFLQSSQHIP PSTPAQKLRK KNNFDSFRIS NSHISPFASG SFSPFATSSP NFLSTSTPAP PNSNNANPST LFSSIPSSRH TTSNHFPSNS AQSSLFSPTA RPLTARKLGF ASSQTKSAVS NNHSRNSSKD ASFMMKSFIP SNRSHPQTQQ NESSLFSDNS MVNSSSNSFS LFPNATLPNP PSSELLTTPF QQIKPPSQVF MSTGLLSKQH RPRKNINFTP LPPSTPSKPS TFVRPHSSST DSPPSPSTPS NTQTDSYFIQ RENTPTNHNS IPTIQLEKSS MDFLRFDPPP SAVKTSHNYG LPFLSNQRCP ATPTRNPFAF ENTVSIHMDG RQPSPIKSRN NNQMSFAMEE EADVSQPSSS SFTLSFPSAL TSSKVSSSTS HLLTRFRNVT LLGSGEFSEV FQVEDPVEKT LKYAVKKLKV KFSGPKERNR LLQEVSIQRA LKGHDHIVEL MDSWEHGGFL YMQVELCENG SLDRFLEEQG QLSRLDEFRV WKILVEVALG LQFIHHKNYV HLDLKPANVM ITFEGTLKIG DFGMASVWPV PRGMEREGDC EYIAPEVLAN HLYDKPADIF SLGITVFEAA ANIVLPDNGQ SWQKLRSGDL SDAPRLSSTD NGSSLTSSSR ETPANSIIGQ GGLDRVVEWM LSPEPRNRPT IDQILATDEV CWVEMRRKAG AIIYEGIHGS SSNPQGDQMM EDWQVNV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.

Gene References into Functions

Regulation of Wee1 localization around the spindle pole body during the G2/M transition is important for proper mitotic entry and progression. PMID:21233285
in fission yeast Wee1 is the major inhibitory kinase regulating CDK activity in S phase. PMID:26918805
This cell cycle arrest is dependent on the Wee1 tyrosine kinase inhibiting the key cell cycle regulator, CDK1/Cdc2. Mutant cells lacking Wee1 are not arrested at G2 upon glucose limitation and lose viability faster than the wild-type cells under glucose-depleted quiescent conditions, suggesting that this cell cycle arrest is required for extension of chronological lifespan PMID:26804466
Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitrosative stress. PMID:26006103
Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase. PMID:24006256
Substitution of a conserved methionine residue to phenylalanine in Plo1, Orb5 and Wee1 kinases increased the sensitivities to ATP analogues. PMID:23986474
Data show that the protein Skb1 interacted with both Cdr1 and the Cdr1 inhibitory target Wee1. PMID:23615447
Sid2 activation midway through G2 phase promotes Fin1 phosphorylation and mitotic commitment throught pom1/Wee1 cell geometry network. PMID:22684255
in fission yeast Cpc2/RACK1 positively regulates from the ribosome the mitotic onset by modulating both the protein levels and the activity of Wee1 PMID:20974849
The changed division response (Cdr) kinases Cdr1 and Cdr2 are negative regulators of Wee1 and they are required for the Tra1-dependent alterations to Wee1 function. PMID:20194963
findings demonstrate that G2 arrest induced by HIV-1 Vpr is also associated with increase in phosphorylation & amount of Wee1 in a Chk1/Cds1-independent manner; Rad24 appears to contribute to Vpr-induced G2 arrest by elevating Wee1 expression PMID:16968670