wee1 Antibody

Code: CSB-PA357135XA01SXV

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

P07527

Target Names

wee1

Alternative Names

wee1 antibody; SPCC18B5.03Mitosis inhibitor protein kinase wee1 antibody; EC 2.7.10.2 antibody; P107 protein kinase homolog antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) wee1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.

Gene References into Functions

Regulation of Wee1 localization around the spindle pole body during the G2/M transition is important for proper mitotic entry and progression. PMID:21233285
in fission yeast Wee1 is the major inhibitory kinase regulating CDK activity in S phase. PMID:26918805
This cell cycle arrest is dependent on the Wee1 tyrosine kinase inhibiting the key cell cycle regulator, CDK1/Cdc2. Mutant cells lacking Wee1 are not arrested at G2 upon glucose limitation and lose viability faster than the wild-type cells under glucose-depleted quiescent conditions, suggesting that this cell cycle arrest is required for extension of chronological lifespan PMID:26804466
Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitrosative stress. PMID:26006103
Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase. PMID:24006256
Substitution of a conserved methionine residue to phenylalanine in Plo1, Orb5 and Wee1 kinases increased the sensitivities to ATP analogues. PMID:23986474
Data show that the protein Skb1 interacted with both Cdr1 and the Cdr1 inhibitory target Wee1. PMID:23615447
Sid2 activation midway through G2 phase promotes Fin1 phosphorylation and mitotic commitment throught pom1/Wee1 cell geometry network. PMID:22684255
in fission yeast Cpc2/RACK1 positively regulates from the ribosome the mitotic onset by modulating both the protein levels and the activity of Wee1 PMID:20974849
The changed division response (Cdr) kinases Cdr1 and Cdr2 are negative regulators of Wee1 and they are required for the Tra1-dependent alterations to Wee1 function. PMID:20194963
findings demonstrate that G2 arrest induced by HIV-1 Vpr is also associated with increase in phosphorylation & amount of Wee1 in a Chk1/Cds1-independent manner; Rad24 appears to contribute to Vpr-induced G2 arrest by elevating Wee1 expression PMID:16968670