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Recombinant Saccharomyces cerevisiae Lysine--tRNA ligase, mitochondrial (MSK1), partial

Recombinant Saccharomyces cerevisiae Lysine--tRNA ligase, mitochondrial (MSK1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MSK1

Uniprot NO.

P32048

Alternative Names

MSK1; YNL073W; N2364; Lysine--tRNA ligase; mitochondrial; EC 6.1.1.6; Lysyl-tRNA synthetase; LysRS

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

Yeast

Expression Region

31-576

Target Protein Sequence

LAHAVDTSKM EATRRNGQIV KDLGRYYPSM SESALHDLCQ EYKEVTIADF NERFLGNPAT LHHEDNPNLL LSINGRIKSI RFSGQKIVFI DLYNGSSGLK NDTQLQLIVN YNKIGGSSED KANFSEYMNF LKKGDYIKAL GYPGFSQSRV KMLSLICNKL PIVLSVSQLP LPSRLNDETK IKSNRVVDYQ LNGTQTLLVR ARIIKLLRKF LDDRNFVEVE TPILSSKSNG AMAKPFITSS KDFDHLELRI APELWLKRLI ISGLQKVYEI GKVFRNEGID STHNAEFSTL EFYETYMSMD DIVTRTEDLF KFLITNLQKF FQDTRLPVPK TFSELHLALS ENNWKFRKVE FLPTLNKELG IDLMNSGLDI NKPSELLKAL PKDIAKKYFP SADNTGQLSS LQILNKLSDV FLEQRHCQST LPTVIYHQPA ILSPLAKTDP QNKQVTKRFE VFIKGKEYIN AYEEENCPQL QLQKFLQQKQ INELTGNKTE TLSPVIDYQY VETMKYGMPP VGGFGLGIDR LCMLFCDKKR IEEVLPFGCV DDVNRQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Gene References into Functions

one out of two cytosolic lysine tRNAs of the yeast Saccharomyces cerevisiae is partially internalized by mitochondria. The key protein factor of this process is the precursor of mitochondrial lysyl-tRNA synthetase, preMsk1p. PMID:27908233
In this work, the authors identify the hinge region with two adjacent helices H5 and H7 of preMSK1 to be responsible for mitochondrial targeting of the tRNA(Lys) and characterize preMsk1p versions with altered tRK1 import capacities. PMID:20064631

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Subcellular Location

Mitochondrion matrix.

Protein Families

Class-II aminoacyl-tRNA synthetase family

Database Links

KEGG: sce:YNL073W

STRING: 4932.YNL073W

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