Home
Product
Protein
Recombinant Proteins
Recombinant Mouse NAD-dependent protein deacylase sirtuin-5, mitochondrial (Sirt5)

Recombinant Mouse NAD-dependent protein deacylase sirtuin-5, mitochondrial (Sirt5)

Mammalian cell

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Abbreviation

Sirt5

Purity

>85% (SDS-PAGE)

Target Names

Sirt5

Uniprot NO.

Q8K2C6

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

37-310

Target Protein Sequence

SSNM ADFRKCFANA KHIAIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPQA FARNPSQVWE FYHYRREVMR SKEPNPGHLA IAQCEARLRD QGRRVVVITQ NIDELHRKAG TKNLLEIHGT LFKTRCTSCG TVAENYRSPI CPALAGKGAP EPETQDARIP VDKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA LCDLCLVVGT SSVVYPAAMF APQVASRGVP VAEFNMETTP ATDRFRFHFP GPCGKTLPEA LAPHETERTS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox.

Gene References into Functions

Data indicate that sirtuin 5 (SIRT5) is an important mitochondrial enzyme for protection against metabolic and ischemic stress following protein kinase C epsilon (PKCepsilon) activation in the brain. PMID:27435822
findings reveal a key role for SIRT5 in maintaining cardiac oxidative metabolism under pressure overload to ensure survival PMID:28972174
Deletion of Sirt5 in starved mouse embryonic fibroblasts increased levels of mitochondrial dynamics leading to mitochondrial accumulation of the pro-fission Drp1 and to mitochondrial fragmentation. PMID:28340937
Our study uncovers a SIRT5-dependent mechanism that regulates cellular NADPH homeostasis and redox potential by promoting IDH2 desuccinylation and G6PD deglutarylation. PMID:27113762
Data (including data from studies using knockout mice) suggest that SIRT5 is targeted to protein complexes on the inner mitochondrial membrane via affinity for cardiolipin to promote respiratory chain function, particularly Complex I and Complex II; SIRT5 expression is observed in inner mitochondrial membrane of periportal hepatocytes. PMID:28458255
Data show that peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha) overexpression significantly increased the expression of sirtuin 3 (SIRT3) and sirtuin 5 (SIRT5). PMID:27052737
In the cochlea, the expression of SIRT1, 3, and 5 (both mRNA and protein) was decreased in the old mice PMID:26472659
These findings establish that regulating heart metabolism and function is a major physiological function of lysine succinylation and SIRT5. PMID:27051063
SIRT5 has a role in cellular metabolism with a multiple enzymatic activities PMID:26208827
SIRT3 and SIRT5 regulate the enzyme activity and cardiolipin binding of very long-chain acyl-CoA dehydrogenase PMID:25811481
Pathway analysis identified glycolysis as the top SIRT5-regulated pathway. Importantly, glycolytic flux was diminished in primary hepatocytes from Sirt5(-/-) compared to WT mice. PMID:26073543
Data indicated that SIRT5 ameliorates MPTP-induced nigrostriatal dopaminergic degeneration via preserving mitochondrial antioxidant capacity. PMID:25541039
Lysine glutarylation is a protein posttranslational modification regulated by SIRT5. PMID:24703693
Loss of SIRT5 leads to accumulation of medium- and long-chain acylcarnitines and decreased beta-hydroxybutyrate production in vivo. PMID:24315375
Sirt5 is likely to be dispensable for the metabolic homeostasis under the basal conditions. PMID:24076663
We have isolated and characterized the murine sirt5 genomic sequence, which spans a region of 24,449 bp and which has one single genomic locus. PMID:23673559
SIRT5 represses biochemical activity of, and cellular respiration through, two protein complexes identified as pyruvate dehydrogenase complex and succinate dehydrogenase. PMID:23806337
The results suggest that SIRT5 activates UOX through deacetylation in mouse liver mitochondria. PMID:23085393
These data indicate SIRT5 also has a role in the metabolic adaptation to fasting, high protein diet and calorie restriction. PMID:20157539
Because ammonia generated during fasting is toxic, SIRT5 protein might play a protective role by converting ammonia to non-toxic urea through deacetylation and activation of CPS1. PMID:20097174
results suggest that the SIRT3, SIRT4, and SIRT5 proteins exert distinct functions in mitochondria. PMID:18054327
SIRT5 plays a pivotal role in ammonia detoxification and disposal by activating carbamoyl phosphate synthetase 1 PMID:19410549

Hide All

Subcellular Location

Mitochondrion. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.

Protein Families

Sirtuin family, Class III subfamily

Tissue Specificity

Detected in brain, liver, heart, kidney, lung, thymus, spleen, skeletal muscle, intestine, pancreas and testis (at protein level).

Database Links

UNIGENE: Mm.35325

KEGG: mmu:68346

STRING: 10090.ENSMUSP00000071048

Promotions