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Recombinant Mouse Isocitrate dehydrogenase [NADP] Cytoplasmic domain (Idh1), partial

Recombinant Mouse Isocitrate dehydrogenase [NADP] Cytoplasmic domain (Idh1), partial

Code: CSB-EP010989MO

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Size:

20μg100μg1mg(1mg*1 or 500ug*2)

US$306

Quantity:

Express system: E.coli

Species: Mus musculus (Mouse)

Tag Info: N-terminal 6xHis-tagged

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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Idh1

Uniprot NO.

O88844

Research Area

Others

Alternative Names

Idh1; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; EC 1.1.1.42; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

7-407aa

Target Protein Sequence

GGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPKDGTQKVTYMVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKKYKSQFEAQKICYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNTELSFFAKALEDVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

49.2kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Troubleshooting and FAQs

Protein FAQs

Datasheet & COA

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Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

Vitamin C treatment induced an IDH1(R132H)-dependent reduction in cell proliferation. PMID:28663574
IDH1 expression regulates TCA cycle in glioma and affects glioma cell growth.GDH2 promotes growth of IDH1-expressing gliomas. PMID:29097607
Through the use of a conditional mutant mouse model that confers a less aggressive tumor phenotype, findings provide new insight into the effects of mutant Idh1 on a candidate cell of origin for glioma, mutant Idh1's role in disrupting the microenvironment from which gliomas arise, and mutant Idh1's effect on the course of glioma progression. PMID:28148827
IDH1 mutations caused down-regulation of leukocyte chemotaxis, resulting in repression of the tumor-associated immune system. PMID:28465358
Idh1(R132H) mutation in the major adult neurogenic stem cell niche causes a phenotype resembling gliomagenesis PMID:27693047
Using whole RNA sequencing of bone marrow cells in iron-overloaded mice, it was observed that Idh1 and Aco1, enzymes involved in the TCA cycle, were elevated. PMID:27380194
data suggest that mutant IDH1 contributes to malignancy in the T-cell lineage and may alter the metabolic profile of malignant T cells PMID:26787889
The results suggest that Idh1 has a physiological function in protecting cells from oxidative stress by regulating the intracellular NADP(+)/NADPH ratio. PMID:25882048
These results support the translational potential of immunotherapeutic targeting of gliomas carrying IDH1 mutations R132H. PMID:25849072
MiR-181a regulates lipid metabolism via IDH1 PMID:25739786
revealed a role for IDH1 in the synthesis/turnover of phospholipids in developing astrocytes and highlight the lipid alterations resulting from the loss of wild-type IDH1 activity. PMID:25218477
these data show that mutant IDH or d-2HG causes persistence of chondrocytes, giving rise to rests of growth-plate cells that persist in the bone as enchondromas. PMID:25730874
WA might exert its chemopreventive activity via inhibiting not only oncogenic activation, but also IDH1 inactivation. PMID:23107437
Brain-specific Idh1-KI mice show brain hemorrhage accompanied by high D2HG levels but decreased ROS PMID:22925884
Suggest that decreased IDPc expression renders melanocytes more vulnerable to oxidative stress, and IDPc plays an important antioxidant function in melanocytes. PMID:22264756
Absence of IDH1 mutations in low-grade gliomas (LGGs) identifies a novel entity of LGGs with distinctive location, infiltrative behavior, specific molecular alterations, and dismal outcome. PMID:21080178
a possible association between IDH mutations and trisomy 8 in myelodysplastic syndromes and acute myeloid leukemia. PMID:20861910
IDPc would be a major NADPH producer required for fat and cholesterol synthesis. PMID:15254034
The results suggest that IDPc plays an important protective role in apoptosis of HL-60 cells induced by singlet oxygen. PMID:17646934
IDPc is expressed differentially along tubules in patterns that may contribute to differences in susceptibility to injury, major enzyme in cytosolic NADPH generation in kidney, downregulated with ischemia-reperfusion PMID:19106211