Recombinant Mouse Complement component C8 gamma chain (C8g)

Code: CSB-EP823734MO
Size:
20ug
20ug100ug1mg
US$256
Quantity:
Express system: E.coli
Species: Mus musculus (Mouse)
Tag Info: C-terminal 6xHis-tagged
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Product Details

Abbreviation
Recombinant Mouse C8g protein
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Target Names
C8g
Uniprot NO.
Research Area
Immunology
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
21-202aa
Target Protein Sequence
QRTRKPIGSTSPISTIQAQVNFSAQKFAGTWLLVAVGSSCRFLQEQGHRAEATTLHAAPQGAAMAVSTFRKLDGICWQVRQLFENTGVPGRFLFQVSRARGPVHMVVAETDYQSFAILYLEQGRKLSVKLYVRSLPVNDSVLDVFERRVREANLTEDQILFFPKYGFCETADQLHILNEVPR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
27.4 kDa
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Troubleshooting and FAQs
Datasheet & COA
Please contact us to get it.
Images
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Customer Reviews and Q&A

 Customer Reviews

Target Background

Function(From Uniprot)
C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.
Gene References into Functions
  1. A review of the human C8 gamma ortholog. PMID:11058761
  2. This is a study of the human C8 orthologs. Binding of C8 gamma subunit to C8 beta is dependent on the thrombospondin type 1 module, low-density lipoprotein receptor class A module, and membrane attack complex/perforin domain of C8 beta. PMID:12220191
  3. This is a study of the human orthologs of C8 alpha, beta, and gamma. C8 gamma is not required for complement-mediated killing of Gram-negative bacteria. PMID:12413696
  4. Results describe the crystal structure of the human ortholog C8 alpha MACPF domain disulfide-linked to C8 gamma (alphaMACPF-gamma) at 2.15 A resolution. PMID:18440555

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Subcellular Location
Secreted.
Protein Families
Calycin superfamily, Lipocalin family
Database Links
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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