Abbreviation
Recombinant Human RGMA protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human RGMA at 2 μg/mL can bind Anti-RGMA recombinant antibody (CSB-RA836186MA1HU). The EC50 is 0.2160-0.4838 ng/mL.
Research Area
Neuroscience
Alternative Names
Repulsive guidance molecule A; RGM domain family member A
Species
Homo sapiens (Human)
Expression Region
48-422aa
Target Protein Sequence
CKILKCNSEFWSATSGSHAPASDDTPEFCAALRSYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human RGMA at 2 μg/ml can bind Anti-RGMA recombinant antibody (CSB-RA836186MA1HU). The EC50 is 0.2160-0.4838 ng/mL.
Description
RGMA plays a critical role in axonal guidance and neuronal development, and its dysregulation has been implicated in cancer progression and immune modulation. This mammalian-expressed protein spanning residues 48–422 demonstrates robust receptor-binding activity, with an EC50 of 0.2160–0.4838 ng/mL in functional ELISA against anti-RGMA recombinant antibody, providing a quantitative benchmark for receptor-ligand interaction studies and antibody characterization assays. The mammalian expression system preserves native glycosylation patterns essential for proper folding and biological activity, making this protein appropriate for surface plasmon resonance binding kinetics, competitive ELISA development, and functional blocking studies in neuronal differentiation models. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this preparation meets the quality thresholds commonly required for cell-based assays investigating RGMA's role in axon repulsion, neogenin signaling, and tumor microenvironment interactions.
