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Recombinant Human Protein arginine N-methyltransferase 2 (PRMT2)

Recombinant Human Protein arginine N-methyltransferase 2 (PRMT2)

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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PRMT2

Uniprot NO.

P55345

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

1-433

Target Protein Sequence

MATSGDCPRS ESQGEEPAEC SEAGLLQEGV QPEEFVAIAD YAATDETQLS FLRGEKILIL RQTTADWWWG ERAGCCGYIP ANHVGKHVDE YDPEDTWQDE EYFGSYGTLK LHLEMLADQP RTTKYHSVIL QNKESLTDKV ILDVGCGTGI ISLFCAHYAR PRAVYAVEAS EMAQHTGQLV LQNGFADIIT VYQQKVEDVV LPEKVDVLVS EWMGTCLLFE FMIESILYAR DAWLKEDGVI WPTMAALHLV PCSADKDYRS KVLFWDNAYE FNLSALKSLA VKEFFSKPKY NHILKPEDCL SEPCTILQLD MRTVQISDLE TLRGELRFDI RKAGTLHGFT AWFSVHFQSL QEGQPPQVLS TGPFHPTTHW KQTLFMMDDP VPVHTGDVVT GSVVLQRNPV WRRHMSVALS WAVTSRQDPT SQKVGEKVFP IWR

Protein Length

Full length protein

Tag Info

N-terminal His-tagged/Tag-Free

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Description

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Target Background

Function

Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.

Gene References into Functions

Results show that tamoxifen resistance in breast cancer cells is connected with the downregulation of PRMT2 and the upregulation of ER-alpha36. Moreover, PRMT2 was able to interact with ER-alpha36 directly, suppress ER-alpha36 and downstream PI3K/Akt and MAPK/ERK signaling, reversing the tamoxifen resistance of breast cancer cells. PMID:29620287
Low splice variant of PRMT2 expression is associated with breast cancer. PMID:28677794
Cobl-mediated dendritic arborization required PRMT2, complex formation with PRMT2, and PRMT2's catalytic activity PMID:29689199
The PRMT2 interacts with SAM68 in cells and regulates its subcellular localization via the SH3 domain of PRMT2, prompting us to investigate the potential role of PRMT2 in BCL-X alternative splicing. PMID:28057797
This study demonstrates the role and significant correlation between a histone methyltransferase (PRMT2)-dependent signature, RORgamma, the cell-cycle regulation, DNA repair circuits, and breast cancer survival outcomes. PMID:24911119
This study uncovers the molecular mechanism of PRMT2 regulating the expression of CCND1 in invasive ductal breast carcinoma. PMID:24292672
characterization of 3 PRMT2 splice variants (PRMT2alpha, PRMT2beta, and PRMT2gamma) from breast cancer; these variants bind to ERalpha in vitro/in vivo; overall PRMT2 expression is upregulated in breast cancer and associated w/ ERalpha positivity PMID:22093364
Data suggest that post-transcriptional processing mechanism as alternative polyadenylation and splicing may play a crucial role in regulating human PRMT2 gene expression. PMID:21820040
the SH3 domain may mediate an interaction between PRMT1 and -2 in a methylation-dependent fashion PMID:21851090
PRMT2 is a novel ERalpha coactivator. PMID:12039952
PRMT2 inhibits NF-kappa B function and promotes apoptosis. PMID:16648481
ligand-dependent AR conformation is essential for the recruitment and nuclear translocation of PMRT2 which acts as AR-coactivator, presumably by arginine methylation. PMID:17587566
Here we report methylation activity from PRMT2 and compare it with PRMT1 activity using UPLC-MS/MS (ultra-performance liquid chromatography-tandem MS), gel electrophoresis, and thin-layer chromatography PMID:19405910

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Subcellular Location

[Isoform 1]: Cytoplasm. Nucleus. Note=Translocates from the cytoplasm to the nucleus, after hormone exposure. Excluded from nucleolus.; [Isoform PRMT2Alpha]: Nucleus. Note=Excluded from nucleolus.; [Isoform PRMT2Beta]: Cytoplasm. Nucleus. Nucleus, nucleolus.; [Isoform PRMT2Gamma]: Nucleus. Note=Excluded from nucleolus.; [Isoform PRMT2L2]: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family

Tissue Specificity

Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.

Database Links

HGNC: 5186

UNIGENE: Hs.154163

KEGG: hsa:3275

STRING: 9606.ENSP00000347906

OMIM: 601961

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