PRMT2 Antibody

Code: CSB-PA018732ESR1HU

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20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC

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Target Background

Product Details

Uniprot NO.

P55345

Target Names

PRMT2

Alternative Names

ANM2_HUMAN antibody; EC 2.1.1. antibody; histone arginine N methyltransferase PRMT2 antibody; Histone-arginine N-methyltransferase PRMT2 antibody; HMT 1 antibody; HMT1 (hnRNP methyltransferase S. cerevisiae) like 1 antibody; HMT1 antibody; HMT1 hnRNP methyltransferase like 1 antibody; hnRNP methyltransferase (S. cerevisiae) like 1 antibody; hnRNP methyltransferase like 1 antibody; Hrmt1l1 antibody; MGC111373 antibody; PRMT 2 antibody; PRMT2 alpha antibody; prmt2 antibody; PRMT2 beta antibody; PRMT2 gamma antibody; PRMT2 protein antibody; PRMT2L2 antibody; Protein arginine methyltransferase 2 antibody; Protein arginine N methyltransferase 2 antibody; Protein arginine N-methyltransferase 2 antibody; Zf2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein arginine N-methyltransferase 2 protein (1-300AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:200-1:2000
IHC	1:20-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: PRMT2 antibody at 4µg/ml + HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 50, 32, 38, 33, 34, 26, 32 kDa
Observed band size: 50 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA018732ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018732ESR1HU at dilution of 1:100

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Target Background

Function(From Uniprot)

Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.

Gene References into Functions

Results show that tamoxifen resistance in breast cancer cells is connected with the downregulation of PRMT2 and the upregulation of ER-alpha36. Moreover, PRMT2 was able to interact with ER-alpha36 directly, suppress ER-alpha36 and downstream PI3K/Akt and MAPK/ERK signaling, reversing the tamoxifen resistance of breast cancer cells. PMID:29620287
Low splice variant of PRMT2 expression is associated with breast cancer. PMID:28677794
Cobl-mediated dendritic arborization required PRMT2, complex formation with PRMT2, and PRMT2's catalytic activity PMID:29689199
The PRMT2 interacts with SAM68 in cells and regulates its subcellular localization via the SH3 domain of PRMT2, prompting us to investigate the potential role of PRMT2 in BCL-X alternative splicing. PMID:28057797
This study demonstrates the role and significant correlation between a histone methyltransferase (PRMT2)-dependent signature, RORgamma, the cell-cycle regulation, DNA repair circuits, and breast cancer survival outcomes. PMID:24911119
This study uncovers the molecular mechanism of PRMT2 regulating the expression of CCND1 in invasive ductal breast carcinoma. PMID:24292672
characterization of 3 PRMT2 splice variants (PRMT2alpha, PRMT2beta, and PRMT2gamma) from breast cancer; these variants bind to ERalpha in vitro/in vivo; overall PRMT2 expression is upregulated in breast cancer and associated w/ ERalpha positivity PMID:22093364
Data suggest that post-transcriptional processing mechanism as alternative polyadenylation and splicing may play a crucial role in regulating human PRMT2 gene expression. PMID:21820040
the SH3 domain may mediate an interaction between PRMT1 and -2 in a methylation-dependent fashion PMID:21851090
PRMT2 is a novel ERalpha coactivator. PMID:12039952
PRMT2 inhibits NF-kappa B function and promotes apoptosis. PMID:16648481
ligand-dependent AR conformation is essential for the recruitment and nuclear translocation of PMRT2 which acts as AR-coactivator, presumably by arginine methylation. PMID:17587566
Here we report methylation activity from PRMT2 and compare it with PRMT1 activity using UPLC-MS/MS (ultra-performance liquid chromatography-tandem MS), gel electrophoresis, and thin-layer chromatography PMID:19405910

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Subcellular Location

[Isoform 1]: Cytoplasm. Nucleus. Note=Translocates from the cytoplasm to the nucleus, after hormone exposure. Excluded from nucleolus.; [Isoform PRMT2Alpha]: Nucleus. Note=Excluded from nucleolus.; [Isoform PRMT2Beta]: Cytoplasm. Nucleus. Nucleus, nucleolus.; [Isoform PRMT2Gamma]: Nucleus. Note=Excluded from nucleolus.; [Isoform PRMT2L2]: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family

Tissue Specificity

Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.

Database Links

HGNC: 5186

UNIGENE: Hs.154163

KEGG: hsa:3275

STRING: 9606.ENSP00000347906

OMIM: 601961

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