Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Iboctadekin
Interferon gamma-inducing factor
Species
Homo sapiens (Human)
Expression Region
1-193aa
Target Protein Sequence
MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full?Length
Tag Info
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant human IL18 protein is encoded by a recombinant DNA that was cloned into the expression vector and then transfected into the yeast cells that support the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag gene to the gene fragment coding for the 1-193aa of the human IL18 protein. After purification, the product is the recombinant human IL18 protein. This recombinant IL18 protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This IL18 protein ran to the molecular weight of about 25 kDa under SDS-PAGE condition. It may find use in immunology research.
Interleukin-18 (IL-18) belongs to the IL-1 family of cytokines. The inactive IL-18 precursor is synthesized as its active form by caspase-1. In humans, increased disease severity can be associated with an imbalance of IL-18. IL-18 has been found to be associated with the injury of different organs. Furthermore, the overly imbalance of cytokines could cause potentially fatal conditions such as a cytokine storm. IL-18 forms a signaling complex by binding to the IL-18 alpha chain. It has been reported that IL-18 is associated with hyperphagia and a variety of metabolic syndrome. Importantly, increasing numbers of animal and clinical studies indicate a role for IL-18 in heart disease.
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