Abbreviation
Recombinant Human IL-18 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
≤10 EU/mg by the LAL method
Activity
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is ≤2.0 ng/mL.
Alternative Names
Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma;GIF; IL-18; IL-1g; IL1F4; MGC12320
Species
Homo sapiens (Human)
Expression Region
37-193aa
Target Protein Sequence
YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
IL-18 plays a central role in Th1 polarization by synergizing with IL-12 to drive IFN-γ production, yet its activity is notoriously sensitive to endotoxin contamination that can confound immune cell readouts. This tag-free recombinant human IL-18, spanning the full mature protein sequence (aa 37–193), demonstrates potent bioactivity with an ED50 ≤2.0 ng/mL in inducing IFN-γ secretion by KG-1 cells co-stimulated with TNF-α, providing a quantitative benchmark for dose-response studies in Th1 differentiation and macrophage activation assays. Endotoxin levels ≤10 EU/mg satisfy the stringent criteria expected for primary immune cell cultures, PBMC-based functional assays, and in vivo inflammation models where LPS artifacts must be minimized. Purity exceeding 95% by SDS-PAGE, combined with the validated signaling potency, supports use in JAK/STAT and NF-κB pathway analysis, as well as antibody development workflows requiring a functional immunogen or ELISA standard.
