Abbreviation
Recombinant Human INHBA protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 0.9519-1.213 ng/mL.
②Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Human ACVR2A (CSB-MP001260HU1d9).The EC50 is 74.75-84.73 ng/mL.
③Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Human ACVR2B (CSB-MP623829HUi9).The EC50 is 45.93-52.42 ng/mL.
④Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Anti-INHBA recombinant antibody (CSB-RA011719MA1HU). The EC50 is 10.28-12.68 ng/mL.
Alternative Names
Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein (EDF); INHBA
Species
Homo sapiens (Human)
Expression Region
21-426aa
Target Protein Sequence
SPTPGSEGHSAAPDCPSCALAALPKDVPNSQPEMVEAVKKHILNMLHLKKRPDVTQPVPKAALLNAIRKLHVGKVGENGYVEIEDDIGRRAEMNELMEQTSEIITFAESGTARKTLHFEISKEGSDLSVVERAEVWLFLKVPKANRTRTKVTIRLFQQQKHPQGSLDTGEEAEEVGLKGERSELLLSEKVVDARKSTWHVFPVSSSIQRLLDQGKSSLDVRIACEQCQESGASLVLLGKKKKKEEEGEGKKKGGGEGGAGADEEKEQSHRPFLMLQARQSEDHPHRRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Activity
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 0.9519-1.213 ng/mL.
-
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/ml can bind Human ACVR2A (CSB-MP001260HU1d9).The EC50 is 74.75-84.73 ng/mL.
-
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/ml can bind Human ACVR2B (CSB-MP623829HUi9).The EC50 is 45.93-52.42 ng/mL.
-
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/ml can bind Anti-INHBA recombinant antibody (CSB-RA011719MA1HU). The EC50 is 10.28-12.68 ng/mL.
Description
Activin A, the homodimer formed by INHBA, regulates cell fate decisions in hematopoiesis, mesoderm patterning, and reproductive physiology, making quantitative receptor-binding data essential for interpreting dose-dependent signaling outcomes. This full-length protein (aa 21–426) demonstrates an ED50 of 0.95–1.21 ng/mL in MPC-11 proliferation assays and binds ACVR2A with an EC50 of 74.75–84.73 ng/mL and ACVR2B with an EC50 of 45.93–52.42 ng/mL, providing defined potency benchmarks for receptor-ligand competition assays, stem cell differentiation studies, and erythroid lineage commitment experiments. Mammalian expression preserves native glycosylation patterns critical for receptor engagement, supporting use in functional ELISA platforms and antibody characterization workflows where post-translational fidelity directly impacts binding kinetics. Purity exceeding 95% and endotoxin levels below 1.0 EU/μg satisfy the quality thresholds commonly required for cell-based proliferation assays and primary culture differentiation protocols.
