Abbreviation
Recombinant Human Activin A protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
≤10 EU/mg by the LAL method
Activity
Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10^5 units/mg.
Alternative Names
Activin beta-A chain;Erythroid differentiation protein ;EDF
Species
Homo sapiens (Human)
Expression Region
311-426aa
Target Protein Sequence
GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered solution containing 0.085%TFA,30%ACN, 5% mannitol, pH 2.5
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
Activin A, a homodimer of the inhibin beta A chain, plays a central role in regulating cell proliferation, differentiation, and apoptosis across diverse tissue contexts, making it a critical tool for dissecting TGF-β superfamily signaling. This tag-free preparation, expressed in mammalian cells to preserve native glycosylation, spans the full mature protein sequence (aa 311–426) and demonstrates potent bioactivity in a mouse MPC-11 proliferation assay, with an ED50 ≤ 2.0 ng/ml corresponding to a specific activity ≥ 5 × 10⁵ units/mg—quantitative evidence that supports its use in cell proliferation and survival assays, stem cell differentiation protocols (including neuronal and osteogenic lineages), and receptor-ligand binding studies by ELISA or surface plasmon resonance. Purity exceeding 95% by SDS-PAGE and endotoxin levels ≤ 10 EU/mg align with standards expected in sensitive primary cell cultures and in vivo regenerative medicine models. The mammalian expression system ensures post-translational modifications essential for high-affinity receptor engagement, providing a suitable basis for antibody characterization and as an ELISA standard in developmental biology and tumor microenvironment research.
