Recombinant Human Hyaluronidase PH-20 (SPAM1)

Baculovirus

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SPAM1

Uniprot NO.

P38567

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

36-490

Target Protein Sequence

LNFRA PPVIPNVPFL WAWNAPSEFC LGKFDEPLDM SLFSFIGSPR INATGQGVTI FYVDRLGYYP YIDSITGVTV NGGIPQKISL QDHLDKAKKD ITFYMPVDNL GMAVIDWEEW RPTWARNWKP KDVYKNRSIE LVQQQNVQLS LTEATEKAKQ EFEKAGKDFL VETIKLGKLL RPNHLWGYYL FPDCYNHHYK KPGYNGSCFN VEIKRNDDLS WLWNESTALY PSIYLNTQQS PVAATLYVRN RVREAIRVSK IPDAKSPLPV FAYTRIVFTD QVLKFLSQDE LVYTFGETVA LGASGIVIWG TLSIMRSMKS CLLLDNYMET ILNPYIINVT LAAKMCSQVL CQEQGVCIRK NWNSSDYLHL NPDNFAIQLE KGGKFTVRGK PTLEDLEQFS EKFYCSCYST LSCKEKADVK DTDAVDVCIA DGVCIDAFLK PPMETEEPQI FYNASPSTLS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

Store at -20°C, for extended storage, conserve at -20°C or -80°C.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Title: Inquiry of protein expression in Mammalian cell
Sequence:

http://www.genome.jp/dbget-bin/www_bget?dr:D06604

1. We want to express this sequence, please send a quotation.
2. What is the endotoxin level when the customer receive protein?
3. To what extent do you usually remove endotoxin in in vivo experiments?
4. Can you offer stability test after protein expressinon ? If it is right, please let me know the price.

Thanks for your email. We have the following protein.
Recombinant Human Hyaluronidase PH-20(SPAM1)
CSB-MP022471HU >> Mammalian cell
You can view the product information on this page in details.
In addition, we have another partial protein to recommend:
Recombinant Human Hyaluronidase PH-20(SPAM1),partial
CSB-YP022471HU1 >> Yeast
CSB-EP022471HU1 >> E.coli
CSB-BP022471HU1 >> Baculovirus
CSB-MP022471HU1 >> Mammalian cell
Expression Region: 36-482aa；partial
Tag information: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows: EP,BP,MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged；YP:N-terminal 6xHis-tagged
Sequence:

1. We could provide the endotoxin removal service for free. And you will receive the protein with entotoxin <1.0 EU/ug.
2. At present, we do not have a detailed stability test for individual proteins. From our current preservation situation, the liquid form protein (with 50% glycerol) could keep at -20℃/-80℃ for about 6 months.The lyophilized powder could be stored at -20°C/-80°C for about 12 months. If there is any instability occur during this period, we can provide complete after-sales services.

Could you please let me know the concentration ? Can I know it before ordering ?

Thanks for your inquiry. Our normal concentration is 0.1-0.5mg/ml. If you have any request on the concentration, please communicate with us in advance.

Target Background

Function(From Uniprot)

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Gene References into Functions

Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID:25209425
results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID:24778442
HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID:23247813
The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID:23209833
PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID:23463525
SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID:12932297