Human Hyaluronidase PH-20(SPAM1) ELISA kit

Code: CSB-EL022471HU-IS

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: cell culture supernates

Detect Range: 78 pg/mL-5000 pg/mL

Sensitivity: 39 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details
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Target Background

Product Details

Alternative Names

epididymis secretory sperm binding protein Li 96n ELISA Kit; HEL-S-96n ELISA Kit; HYA1 ELISA Kit; HYAL PH-20 ELISA Kit; HYAL PH20 ELISA Kit; Hyal-PH20 ELISA Kit; HYAL1 ELISA Kit; HYAL3 ELISA Kit; HYAL5 ELISA Kit; HYALP_HUMAN ELISA Kit; Hyaluronidase PH-20 ELISA Kit; Hyaluronoglucosaminidase PH-20 ELISA Kit; MGC26532 ELISA Kit; PH-20 Hyaluronidase ELISA Kit; PH20 ELISA Kit; PH20 Hyaluronidase ELISA Kit; SPAG15 ELISA Kit; SPAM-1 ELISA Kit; Spam1 ELISA Kit; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit; Sperm adhesion molecule 1 ELISA Kit; Sperm surface protein PH-20 ELISA Kit; Sperm surface protein PH20 ELISA Kit; zona pellucida binding ELISA Kit

Abbreviation

SPAM1

Uniprot NO.

P38567

Species

Homo sapiens (Human)

Sample Type

cell culture supernates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

39 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This ELISA kit provides quantitative measurement of human SPAM1 (sperm adhesion molecule 1/hyaluronidase PH-20) in research contexts spanning signal transduction and reproductive biology. Because SPAM1 is a GPI-anchored hyaluronidase that can be released from cell surfaces into conditioned media, detecting its soluble form at low concentrations demands purpose-built assay sensitivity.

Detection Range: The 78–5000 pg/mL range captures the low-to-moderate concentrations of shed SPAM1 typically found in cell culture supernates from transfected lines or primary reproductive tract cells, minimizing the need for extensive sample concentration while accommodating higher-expression systems through simple dilution.

Sensitivity: At 39 pg/mL, the kit detects SPAM1 in conditioned media from cells with modest endogenous expression, including epididymal or cumulus cell cultures where soluble PH-20 levels may be near the lower physiological threshold.

Sample Compatibility: Validation for cell culture supernates directly supports in vitro studies of SPAM1 shedding mechanisms, GPI-anchor cleavage regulation, and hyaluronidase activity modulation without requiring cell lysis or membrane fractionation.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate experiments where conditioned media volumes are limited, such as small-scale primary spermatocyte or organoid cultures, enabling same-day quantification across multiple treatment conditions.

This kit is well suited for studies of sperm-oocyte interaction, cumulus matrix remodeling, and SPAM1-mediated signaling in fertilization research.

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Target Background

Function(From Uniprot)

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Gene References into Functions

Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID:25209425
results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID:24778442
HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID:23247813
The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID:23209833
PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID:23463525
SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID:12932297