Abbreviation
Recombinant Human FGFR2 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human FGFR2 at 2 μg/mL can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC50 is 1.066-1.203 ng/mL.
Alternative Names
K-sam;Keratinocyte growth factor receptor
Species
Homo sapiens (Human)
Expression Region
152-378aa
Target Protein Sequence
RAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCVVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTEADAGEYICKVSNYIGQANQSAWLTVLPKQQAPGREKEITASPDYLE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform 3
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human FGFR2 at 2 μg/ml can bind Anti-FGFR2 recombinant antibody (CSB-RA008645MA1HU). The EC50 is 1.066-1.203 ng/mL.
Description
FGFR2 dysregulation drives oncogenesis in gastric, breast, and endometrial cancers, making this receptor a critical target for therapeutic intervention and biomarker discovery. This mammalian-expressed construct spanning residues 152–378 of isoform 3 captures the extracellular ligand-binding domain with native post-translational modifications that preserve conformational epitopes essential for antibody recognition. Functional ELISA validation demonstrates specific binding to an anti-FGFR2 recombinant antibody with an EC50 of 1.066–1.203 ng/mL, confirming that this protein supports competitive inhibition assays, blocking antibody screening, and therapeutic antibody epitope mapping in oncology drug discovery programs. The combination of >95% purity and <1.0 EU/μg endotoxin meets the quality thresholds commonly required for surface plasmon resonance affinity characterization, biolayer interferometry kinetic studies, and high-throughput small-molecule inhibitor screening where low background interference is critical for accurate KD determination.
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