Abbreviation
Recombinant Human DLL4 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human DLL4 at 2 μg/mL can bind Anti-DLL4 recombinant antibody (CSB-RA878862MA1HU). The EC50 is 2.322-2.607 ng/mL.
Alternative Names
Delta-like protein 4; Drosophila Delta homolog 4 (Delta4); DLL4; UNQ1895/PRO4341
Species
Homo sapiens (Human)
Expression Region
27-524aa
Target Protein Sequence
SGVFQLQLQEFINERGVLASGRPCEPGCRTFFRVCLKHFQAVVSPGPCTFGTVSTPVLGTNSFAVRDDSSGGGRNPLQLPFNFTWPGTFSLIIEAWHAPGDDLRPEALPPDALISKIAIQGSLAVGQNWLLDEQTSTLTRLRYSYRVICSDNYYGDNCSRLCKKRNDHFGHYVCQPDGNLSCLPGWTGEYCQQPICLSGCHEQNGYCSKPAECLCRPGWQGRLCNECIPHNGCRHGTCSTPWQCTCDEGWGGLFCDQDLNYCTHHSPCKNGATCSNSGQRSYTCTCRPGYTGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human DLL4 at 2 μg/ml can bind Anti-DLL4 recombinant antibody (CSB-RA878862MA1HU). The EC50 is 2.322-2.607 ng/mL.
Description
Delta-like protein 4 functions as a Notch ligand that drives arterial endothelial specification and regulates angiogenic sprouting, making it a critical target in vascular biology and tumor microenvironment studies. This recombinant fragment spanning residues 27–524 demonstrates quantifiable antibody-binding activity in functional ELISA, with an EC50 of 2.322–2.607 ng/mL when immobilized at 2 μg/mL, confirming that the extracellular domain retains native epitope presentation suitable for antibody development, validation experiments, and as a coating antigen in immunoassays. The endotoxin level below 1.0 EU/μg meets the quality thresholds commonly required for cell-based assays where LPS contamination would confound Notch signaling readouts in endothelial cells, pericytes, or tumor-associated immune populations. Purity exceeding 95% by SDS-PAGE supports use as a reference standard in DLL4 detection platforms and as a positive control in Western blot or flow cytometry protocols interrogating Notch pathway activation.
