Abbreviation
Recombinant Human CLDN18.2 protein, Fluorescent-VLPs (Active)
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 10 μg/mL can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005505MA1HU), the EC50 is 5.115-8.534 ng/mL.The VLPs (CSB-MP3838) is negative control.
Alternative Names
(CLDN18.2)
Species
Homo sapiens (Human)
Expression Region
1-261aa
Target Protein Sequence
MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Isoform A2
Tag Info
C-terminal mCherry-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 10 μg/ml can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005498A1HU), the EC50 is 5.115-8.534 ng/mL. The VLPs (CSB-MP3838) is negative control.
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CSB-MP005498HU(A5)l5 is detected by cherry antibody.
Description
Claudin-18.2 presents a unique challenge for recombinant production because its four-transmembrane topology demands a native membrane context to preserve conformational epitopes critical for antibody recognition. This fluorescent VLP-displayed construct presents full-length human CLDN18.2 (aa 1–261, isoform A2) with a C-terminal mCherry tag, enabling direct visualization in flow cytometry-based antibody screening and cell-based binding assays. Functional ELISA confirms that immobilized CLDN18.2 VLPs bind an anti-CLDN18.2 recombinant antibody with an EC50 of 5.1–8.5 ng/mL, demonstrating preserved extracellular loop conformation suitable for antibody development and validation, therapeutic antibody benchmarking, and tumor metastasis research targeting CLDN18.2-positive cancers. The mammalian-expressed VLP format maintains native folding and membrane orientation while endotoxin levels below 1.0 EU/μg satisfy criteria appropriate for immune cell trafficking studies and integrin-ligand binding assays requiring low background activation.
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